LOCUS       sequence-348481-        9837 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9837)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3164
FEATURES             Location/Qualifiers
     source          1..9837
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2592..2616)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2624..2665
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2775..3363
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2828..2848)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3246..3257)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3435..3668
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3746..3867
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3783..3802)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3837..3856
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3907..4042
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4062..4081)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4063..4081)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4091..4108)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4091..4107)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4100..4122)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4249..4704
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4336..4355)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4546..4567
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4730..4834
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4835..4903,4904..5695)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5053..5072)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5866..6454
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6355..6374
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6608..6625
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6742..6763
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6778..6783,6784..6801,6802..6808)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6816..6832
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6821..6843
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6840..6856
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6840..6856
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6875..6895
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6877..6895
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6923..7149
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7150..7330
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7377..7502
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7995..8228
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8413..8457
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8688..8898
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8710..8906
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8757..8892
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8938..9540
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8938..8957)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9434..9454
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9583..9700
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcatgaagat tgaggaggtg aagagcacta cgaagacgca
     1261 gcgcatcgcc tcccacagcc acgtgaaagg gctggggctg gacgagagcg gcttggccaa
     1321 gcaggcggcc tcagggcttg tgggccagga gaacgcgcga gaggcatgtg gcgtcatagt
     1381 agaattaatc aaaagcaaga aaatggctgg aagagctgtc ttgttggcag gacctcctgg
     1441 aactggcaag acagctctgg ctctggctat tgctcaggag ctgggtagta aggtcccctt
     1501 ctgcccaatg gtggggagtg aagtttactc aactgagatc aagaagacag aggtgctgat
     1561 ggagaacttc cgcagggcca ttgggctgcg aataaaggag accaaggaag tttatgaagg
     1621 tgaagtcaca gagctaactc cgtgtgagac agagaatccc atgggaggat atggcaaaac
     1681 cattagccat gtgatcatag gactcaaaac agccaaagga accaaacagt tgaaactgga
     1741 ccccagcatt tttgaaagtt tgcagaaaga gcgagtagaa gctggagatg tgatttacat
     1801 tgaagccaac agtggggccg tgaagaggca gggcaggtgt gatacctatg ccacagaatt
     1861 cgaccttgaa gctgaagagt atgtcccctt gccaaaaggg gatgtgcaca aaaagaaaga
     1921 aatcatccaa gatgtgacct tgcatgactt ggatgtggct aatgcgcggc cccagggggg
     1981 acaagatatc ctgtccatga tgggccagct aatgaagcca aagaagacag aaatcacaga
     2041 caaacttcga ggggagatta ataaggtggt gaacaagtac atcgaccagg gcattgctga
     2101 gctggtcccg ggtgtgctgt ttgttgatga ggtccacatg ctggacattg agtgcttcac
     2161 ctacctgcac cgcgccctgg agtcttctat cgctcccatc gtcatctttg catccaaccg
     2221 aggcaactgt gtcatcagag gcactgagga catcacatcc cctcacggca tccctcttga
     2281 ccttctggac cgagtgatga taatccggac catgctgtat actccacagg aaatgaaaca
     2341 gatcattaaa atccgtgccc agacggaagg aatcaacatc agtgaggagg cactgaacca
     2401 cctgggggag attggcacca agaccacact gaggtactca gtgcagctgc tgaccccggc
     2461 caacttgctt gctaaaatca acgggaagga cagcattgag aaagagcatg tcgaagagat
     2521 cagtgaactt ttctatgatg ccaagtcctc cgccaaaatc ctggctgacc agcaggataa
     2581 gtacatgaag tgcccaactt tcttgtacaa agtggttgat atcggtaagc ctatccctaa
     2641 ccctctcctc ggtctcgatt ctacgtagta atgaactagt ccgtaattga aagtatttcg
     2701 atttcttggc tttatatatc ttgtggaaag gacgatcaga gcaatatccc ataatcagta
     2761 cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     2821 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     2881 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     2941 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     3001 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     3061 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     3121 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     3181 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     3241 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     3301 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     3361 cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     3421 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     3481 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     3541 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     3601 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     3661 ctctagcagt acgtatagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     3721 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     3781 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     3841 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     3901 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     3961 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     4021 tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt
     4081 acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     4141 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     4201 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta
     4261 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     4321 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     4381 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     4441 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     4501 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     4561 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     4621 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     4681 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     4741 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     4801 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     4861 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     4921 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     4981 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     5041 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     5101 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     5161 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     5221 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     5281 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     5341 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     5401 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     5461 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     5521 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     5581 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     5641 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     5701 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     5761 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     5821 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     5881 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     5941 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     6001 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     6061 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     6121 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     6181 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     6241 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     6301 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     6361 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     6421 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     6481 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     6541 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     6601 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     6661 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     6721 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     6781 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     6841 aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca
     6901 aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg
     6961 gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg
     7021 gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg
     7081 attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga
     7141 tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     7201 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     7261 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     7321 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
     7381 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     7441 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     7501 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     7561 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     7621 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     7681 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     7741 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     7801 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct
     7861 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     7921 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     7981 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     8041 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     8101 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     8161 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     8221 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     8281 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     8341 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     8401 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     8461 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     8521 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     8581 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc catgcattgc
     8641 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     8701 tgcaaagcat gcgtctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     8761 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     8821 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     8881 tttttggagg cctaggcttt tgcaaaaagc tttctagagg taccaccgga gcttaccatg
     8941 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     9001 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     9061 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     9121 ggcaaggtgt gggtcgcgga cgacggcgcc gccgtggcgg tctggaccac gccggagagc
     9181 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     9241 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     9301 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     9361 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     9421 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     9481 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgccagttaa
     9541 cgaattctag atcttgagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     9601 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     9661 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     9721 cagagatcca ctttggcgcc ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa
     9781 acagagagga atctttgcag ctaatggacc ttctaggtct tgaaaggagt gggaatt
//
