LOCUS       sequence-348512-       11149 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11149)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11149)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3166
FEATURES             Location/Qualifiers
     source          1..11149
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3903..3927)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3935..3976
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4087..4675
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4140..4160)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4558..4569)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4747..4980
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5058..5179
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5095..5114)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5149..5168
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5219..5354
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5374..5393)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5375..5393)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5403..5420)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5403..5419)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5412..5434)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5561..6016
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5648..5667)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5858..5879
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6042..6146
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6147..6215,6216..7007)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6365..6384)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7178..7766
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7667..7686
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7920..7937
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8054..8075
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8090..8095,8096..8113,8114..8120)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8128..8144
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8133..8155
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8152..8168
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8152..8168
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8187..8207
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8189..8207
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8235..8461
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8462..8642
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8689..8814
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9307..9540
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9725..9769
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        10000..10210
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        10022..10218
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      10069..10204
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10250..10852
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(10250..10269)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10746..10766
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10895..11012
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcaccatgac ggatcctttc tgcgttggag gccgtcggct
     1261 cccggggtcc agcaagagtg gtcccgggaa ggatggcagc cgaaaggagg tccgacttcc
     1321 aatgctgcat gacccaccga agatggggat gccggtggtc cgtggtggac agacagtgcc
     1381 cggccaggcc cctctctgct ttgacccggg aagtccagcc agtgacaaga cagaagggaa
     1441 gaaaaagggg cggccaaaag ccgagaacca ggccctccga gacattcctc tctccctgat
     1501 gaacgactgg aaggatgagt tcaaggcaca ctcgagggtg aagtgtccaa actcagggtg
     1561 ctggctggag ttccccagca tctacgggct caagtaccat taccagcggt gccaaggggg
     1621 tgccatctca gatcgcctgg ccttcccctg ccccttctgc gaggccgcat tcacctctaa
     1681 gacccagctg gagaaacacc ggatctggaa ccacatggac cgacccctgc ctgcctccaa
     1741 gcctgggccc atcagcaggc cggtcaccat cagccggcct gttggggtca gcaagcccat
     1801 cggagtgagc aaacctgtca ctattggcaa acctgtgggt gtcagcaaac ccattggcat
     1861 cagcaagcca gtctcggtcg gcagacccat gccagtcacc aaggccatcc cggtcactag
     1921 gcccgtgcca gtcaccaaac ctgtcacagt cagcaggccc atgcccgtca ccaaggccat
     1981 gccggtcacc aaacccatca cagtcaccaa gtctgtgccg gtcaccaaac ccgtacctgt
     2041 caccaaaccc attacggtaa caaagcttgt gacagttacg aaacccgtgc cggtcaccaa
     2101 gccagtgaca gtcagcaggc ccattgtggt cagcaagccg gtgacagtca gcaggcccat
     2161 tgctatcagc agacacacac cgccctgcaa aatggtgctg ctgaccaggt cggagaacaa
     2221 agcacctcgt gccacaggga ggaacagtgg taagaaaagg gctgcggaca gcctggacac
     2281 ctgcccaatt ccacccaagc aggccaggcc agagaatggg gagtacggcc cctcctccat
     2341 gggccagagc tcggccttcc agctgagtgc agacaccagc agtggctcct tgtcgccagg
     2401 cagcaggccg tcagggggca tggaggcact gaaggctgca ggccctgcgt ccccgcctga
     2461 ggaggacccg gagcgcacaa agcacagaag gaaacagaaa acacccaaaa agtttacagg
     2521 ggagcagcca tccatctccg ggacctttgg gctcaaaggc ctggtcaaag ctgaggacaa
     2581 ggcccgagtt caccgctcca agaagcagga ggggccaggc cctgaggacg cccggaagaa
     2641 ggtgccagct gcccccatca ctgtcagcaa ggaggcaccg gcccctgtgg cccacccagc
     2701 tccaggtggc cctgaagagc agtggcagag ggccatccat gagcgcgggg aagccgtctg
     2761 ccccacctgc aacgtggtca cccggaagac tctcgtgggg cttaagaagc acatggaggt
     2821 gtgtcagaag cttcaggatg cactcaagtg ccagcactgc cggaagcagt tcaagtccaa
     2881 agccggcctc aactaccaca ctatggccga gcacagtgcc aagccctctg acgccgaggc
     2941 ctccgaaggg ggcgagcagg aggagcgcga gaggctgcgc aaggtgctga agcagatggg
     3001 acggctgcgc tgcccccagg agggttgcgg ggctgccttc tccagcctca tgggctacca
     3061 gtaccaccag cggcgctgcg ggaagccgcc ctgcgaggtg gacagcccct ccttcccctg
     3121 cacccactgt ggcaagacgt accgatccaa ggctggccac gactaccacg tgcgctcgga
     3181 gcacacggcc cccccccctg aggagcccac agacaagtcc cctgaggctg aggacccgct
     3241 gggtgtggag cggaccccaa gcgggcgtgt ccgccgcacg tcggcccagg tggcggtgtt
     3301 ccacctgcag gagatagcgg aggacgagct ggcccgcgac tggaccaagc ggcgcatgaa
     3361 ggatgacctt gtgcccgaga ccgcacggct caactacact cgaccagggc tccccacgct
     3421 gaacccccag ctgctagagg catggaagaa tgaagtgaag gagaaaggcc acgtcaactg
     3481 tcccaacgac tgctgtgaag ccatctactc cagcgtgtcc ggactcaagg ctcatctcgc
     3541 cagctgcagt aagggggccc acctggcagg gaagtaccgc tgtctgctgt gtccgaagga
     3601 gttcagttct gagagtggcg tcaaatacca catcctgaag acccacgcag agaactggtt
     3661 ccgaacatca gcagacccac ctcccaaaca caggagccag gactcattgg tgcccaagaa
     3721 ggaaaagaag aaaaatctgg caggtggaaa gaagcggggc cgaaagccca aggagcggac
     3781 cccagaggag cctgtggcca agctgccccc gcgccgggac gactggcctc caggatgcag
     3841 agacaagggg gcccggggct ccaccggccg gaaggtggga gtcagcaagg cgcctgaaaa
     3901 gttgccaact ttcttgtaca aagtggttga tatcggtaag cctatcccta accctctcct
     3961 cggtctcgat tctacgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg
     4021 gctttatata tcttgtggaa aggacgagct ggtgtttcca aggcatacgt tacgcgttaa
     4081 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     4141 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     4201 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     4261 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     4321 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     4381 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     4441 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     4501 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     4561 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     4621 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga
     4681 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     4741 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     4801 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     4861 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     4921 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     4981 gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     5041 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     5101 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     5161 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     5221 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     5281 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     5341 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     5401 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     5461 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     5521 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     5581 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     5641 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     5701 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     5761 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     5821 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     5881 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     5941 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     6001 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     6061 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     6121 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     6181 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     6241 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     6301 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     6361 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     6421 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     6481 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     6541 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     6601 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     6661 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     6721 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     6781 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     6841 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     6901 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     6961 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     7021 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     7081 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     7141 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     7201 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     7261 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     7321 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     7381 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     7441 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     7501 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     7561 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     7621 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     7681 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     7741 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     7801 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     7861 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     7921 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     7981 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     8041 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     8101 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     8161 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     8221 agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
     8281 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
     8341 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
     8401 aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa
     8461 cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     8521 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     8581 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     8641 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     8701 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     8761 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     8821 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     8881 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     8941 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     9001 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     9061 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     9121 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
     9181 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     9241 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     9301 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     9361 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     9421 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     9481 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     9541 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     9601 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     9661 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     9721 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     9781 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     9841 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     9901 accattatcg tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat
     9961 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
    10021 atgcgtctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
    10081 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
    10141 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
    10201 ggcctaggct tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta
    10261 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
    10321 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
    10381 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
    10441 gtgggtcgcg gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc
    10501 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
    10561 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
    10621 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
    10681 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
    10741 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
    10801 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct
    10861 agatcttgag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
    10921 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
    10981 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
    11041 cactttggcg ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag
    11101 gaatctttgc agctaatgga ccttctaggt cttgaaagga gtgggaatt
//
