LOCUS       sequence-348517-       10759 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10759)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3168
FEATURES             Location/Qualifiers
     source          1..10759
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             2540..2551
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    complement(3513..3537)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3545..3586
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3697..4285
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3750..3770)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4168..4179)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4357..4590
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4668..4789
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4705..4724)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4759..4778
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4829..4964
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4984..5003)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4985..5003)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5013..5030)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5013..5029)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5022..5044)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5171..5626
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5258..5277)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5468..5489
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5652..5756
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5757..5825,5826..6617)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5975..5994)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6788..7376
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7277..7296
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7530..7547
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7664..7685
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7700..7705,7706..7723,7724..7730)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7738..7754
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7743..7765
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7762..7778
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7762..7778
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7797..7817
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7799..7817
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7845..8071
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8072..8252
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8299..8424
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8917..9150
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9335..9379
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9610..9820
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9632..9828
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9679..9814
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9860..10462
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9860..9879)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10356..10376
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10505..10622
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcatgaaaac tagcccccgt cggccactga ttctcaaaag
     1261 acggaggctg ccccttcctg ttcaaaatgc cccaagtgaa acatcagagg aggaacctaa
     1321 gagatcccct gcccaacagg agtctaatca agcagaggcc tccaaggaag tggcagagtc
     1381 caactcttgc aagtttccag ctgggatcaa gattattaac caccccacca tgcccaacac
     1441 gcaagtagtg gccatcccca acaatgctaa tattcacagc atcatcacag cactgactgc
     1501 caagggaaaa gagagtggca gtagtgggcc caacaaattc atcctcatca gctgtggggg
     1561 agccccaact cagcctccag gactccggcc tcaaacccaa accagctatg atgccaaaag
     1621 gacagaagtg accctggaga ccttgggacc aaaacctgca gctagggatg tgaatcttcc
     1681 tagaccacct ggagcccttt gcgagcagaa acgggagacc tgtgcagatg gtgaggcagc
     1741 aggctgcact atcaacaata gcctatccaa catccagtgg cttcgaaaga tgagttctga
     1801 tggactgggc tcccgcagca tcaagcaaga gatggaggaa aaggagaatt gtcacctgga
     1861 gcagcgacag gttaaggttg aggagccttc gagaccatca gcgtcctggc agaactctgt
     1921 gtctgagcgg ccaccctact cttacatggc catgatacaa ttcgccatca acagcactga
     1981 gaggaagcgc atgactttga aagacatcta tacgtggatt gaggaccact ttccctactt
     2041 taagcacatt gccaagccag gctggaagaa ctccatccgc cacaaccttt ccctgcacga
     2101 catgtttgtc cgggagacgt ctgccaatgg caaggtctcc ttctggacca ttcaccccag
     2161 tgccaaccgc tacttgacat tggaccaggt gtttaagcca ctggacccag ggtctccaca
     2221 attgcccgag cacttggaat cacagcagaa acgaccgaat ccagagctcc gccggaacat
     2281 gaccatcaaa accgaactcc ccctgggcgc acggcggaag atgaagccac tgctaccacg
     2341 ggtcagctca tacctggtac ctatccagtt cccggtgaac cagtcactgg tgttgcagcc
     2401 ctcggtgaag gtgccattgc ccctggcggc ttccctcatg agctcagagc ttgcccgcca
     2461 tagcaagcga gtccgcattg cccccaaggt gctgctagct gaggagggga tagctcctct
     2521 ttcttctgca ggaccaggga aagaggagaa actcctgttt ggagaagggt tttctccttt
     2581 gcttccagtt cagactatca aggaggaaga aatccagcct ggggaggaaa tgccacactt
     2641 agcgagaccc atcaaagtgg agagccctcc cttggaagag tggccctccc cggccccatc
     2701 tttcaaagag gaatcatctc actcctggga ggattcgtcc caatctccca ccccaagacc
     2761 caagaagtcc tacagtgggc ttaggtcccc aacccggtgt gtctcggaaa tgcttgtgat
     2821 tcaacacagg gagaggaggg agaggagccg gtctcggagg aaacagcatc tactgcctcc
     2881 ctgtgtggat gagccggagc tgctcttctc agaggggccc agtacttccc gctgggccgc
     2941 agagctcccg ttcccagcag actcctctga ccctgcctcc cagctcagct actcccagga
     3001 agtgggagga ccttttaaga cacccattaa ggaaacgctg cccatctcct ccaccccgag
     3061 caaatctgtc ctccccagaa cccctgaatc ctggaggctc acgcccccag ccaaagtagg
     3121 gggactggat ttcagcccag tacaaacctc ccagggtgcc tctgacccct tgcctgaccc
     3181 cctggggctg atggatctca gcaccactcc cttgcaaagt gctccccccc ttgaatcacc
     3241 gcaaaggctc ctcagttcag aacccttaga cctcatctcc gtcccctttg gcaactcttc
     3301 tccctcagat atagacgtcc ccaagccagg ctccccggag ccacaggttt ctggccttgc
     3361 agccaatcgt tctctgacag aaggcctggt cctggacaca atgaatgaca gcctcagcaa
     3421 gatcctgctg gacatcagct ttcctggcct ggacgaggac ccactgggcc ctgacaacat
     3481 caactggtcc cagtttattc ctgagctaca gtacccaact ttcttgtaca aagtggttga
     3541 tatcggtaag cctatcccta accctctcct cggtctcgat tctacgtagt aatgaactag
     3601 tccgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgacac
     3661 ggcgcgtagg actcgtggca aacgcgttaa gtcgacaatc aacctctgga ttacaaaatt
     3721 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     3781 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     3841 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     3901 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     3961 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     4021 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     4081 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     4141 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     4201 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     4261 atctcccttt gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg
     4321 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     4381 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     4441 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     4501 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     4561 ccttttagtc agtgtggaaa atctctagca gtacgtatag tagttcatgt catcttatta
     4621 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     4681 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     4741 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     4801 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     4861 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     4921 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
     4981 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     5041 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     5101 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     5161 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     5221 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     5281 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     5341 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     5401 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     5461 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     5521 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     5581 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     5641 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     5701 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     5761 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     5821 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     5881 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     5941 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     6001 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     6061 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     6121 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     6181 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     6241 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     6301 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     6361 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     6421 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     6481 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     6541 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     6601 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     6661 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     6721 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     6781 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     6841 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     6901 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     6961 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     7021 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     7081 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     7141 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     7201 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     7261 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     7321 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     7381 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     7441 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     7501 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     7561 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     7621 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     7681 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     7741 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
     7801 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat
     7861 actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa
     7921 aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag
     7981 gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat
     8041 tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc
     8101 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     8161 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     8221 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     8281 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     8341 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     8401 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     8461 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     8521 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     8581 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     8641 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     8701 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     8761 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     8821 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     8881 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     8941 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     9001 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     9061 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     9121 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     9181 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     9241 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     9301 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     9361 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     9421 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     9481 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     9541 cccgagggga cccatgcatt gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     9601 ggctccccag caggcagaag tatgcaaagc atgcgtctca attagtcagc aaccatagtc
     9661 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     9721 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     9781 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctttctaga
     9841 ggtaccaccg gagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     9901 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     9961 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
    10021 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgccgtggc
    10081 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
    10141 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
    10201 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
    10261 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
    10321 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
    10381 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
    10441 ccgcaagccc ggtgccagtt aacgaattct agatcttgag acaaatggca gtattcatcc
    10501 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
    10561 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
    10621 ttcgggttta ttacagggac agcagagatc cactttggcg ccggctcgag gcctgcaggt
    10681 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
    10741 cttgaaagga gtgggaatt
//
