LOCUS       sequence-348520-       10729 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10729)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3170
FEATURES             Location/Qualifiers
     source          1..10729
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3483..3507)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3515..3556
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3667..4255
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3720..3740)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4138..4149)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4327..4560
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4638..4759
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4675..4694)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4729..4748
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4799..4934
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4954..4973)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4955..4973)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4983..5000)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4983..4999)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4992..5014)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5141..5596
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5228..5247)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5438..5459
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5622..5726
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5727..5795,5796..6587)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5945..5964)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6758..7346
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7247..7266
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7500..7517
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7634..7655
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7670..7675,7676..7693,7694..7700)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7708..7724
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7713..7735
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7732..7748
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7732..7748
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7767..7787
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7769..7787
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7815..8041
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8042..8222
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8269..8394
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8887..9120
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9305..9349
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9580..9790
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9602..9798
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9649..9784
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9830..10432
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9830..9849)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10326..10346
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10475..10592
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcatggcgga caagcgcaaa ctccaaggtg agattgatcg
     1261 ctgcctcaag aaggtgtccg agggcgtgga gcagtttgaa gatatttggc agaagctcca
     1321 caatgcagcc aacgcgaacc agaaagaaaa gtatgaggct gacctaaaga aggagattaa
     1381 gaagctacaa cggctgaggg accaaatcaa gacatgggta gcgtccaacg agatcaagga
     1441 caagaggcag cttatagaca accgcaagct cattgagacg caaatggaac ggttcaaagt
     1501 tgtggaacga gagaccaaaa ccaaagctta cagcaaagag ggcctgggcc tggcccagaa
     1561 ggtagatcct gcccagaagg agaaggaaga ggttggccag tggctcacga ataccatcga
     1621 cacgctcaac atgcaggtgg accagtttga gagtgaagtg gagtcactgt cagtgcagac
     1681 acgcaagaag aagggcgaca aggataagca ggaccggatt gagggcttga agcggcacat
     1741 cgagaagcac cgctaccacg tgcgcatgct agagaccatc ctgcgcatgc tggacaatga
     1801 ctccatcctc gttgacgcca tccgcaagat caaggacgac gttgagtact atgttgactc
     1861 atcccaggac cccgacttcg aggagaacga gtttctctac gatgacctgg acctcgagga
     1921 cattccacag gcgctggtcg ccacctcccc ccccagccac agccacatgg aggatgagat
     1981 cttcaaccag tccagcagca cgcccacctc aaccacctcc agctctccca tcccgcccag
     2041 cccagccaac tgtaccacgg aaaactctga agatgataag aagaggggac gttccacaga
     2101 cagtgaagtc agccagtctc cagccaaaaa cggctccaag cctgtccaca gcaaccagca
     2161 ccctcagtcc ccagctgtgc cgcccaccta cccctccggc cccccgcctg ctgcctctgc
     2221 cttgagcacc actcctggca acaatggggt ccccgccccc gcagcacccc caagtgccct
     2281 gggccccaag gccagtccag ctcccagcca caactcgggc acccctgctc cctatgccca
     2341 ggcggtggcc ccaccagctc ccagtgggcc cagcacgacc cagccccggc cccccagcgt
     2401 ccagcctagc ggaggcggag gcggcggcag cggaggcgga gggagcagca gcagtagtaa
     2461 cagcagtgcc ggtggagggg ctggcaagca gaatggcgcc accagttaca gctcagttgt
     2521 ggcagacagc ccggcagagg tggctttgag cagcagtggg ggcaacaatg ccagcagcca
     2581 ggccttgggc cccccttccg gcccccacaa cccacctccc agcacctcga aggaacccag
     2641 tgcggcagcc ccaacggggg ctgggggcgt ggccccaggc tcagggaaca actcaggggg
     2701 acccagcctc ctggtgccac tgcctgtgaa tcctcccagc tccccaacgc ccagcttcag
     2761 tgatgccaag gcagccggtg ccctgctcaa tgggcctcca cagttcagca ccgccccaga
     2821 aatcaaggcc cctgagcctc tgagctcctt gaagtccatg gcggaacggg cagccatcag
     2881 ctctggcatt gaggaccctg tgccaacgct gcacctgacc gagcgagaca tcatcctgag
     2941 cagtacatca gcacctccgg cctcagccca gccgcccctg cagctgtcag aggtgaacat
     3001 accgctgtcg ctgggtgtct gtccactggg ccctgtgccc ctcaccaagg agcagctcta
     3061 tcagcaggcc atggaagagg ccgcctggca ccacatgcct cacccctctg actctgagcg
     3121 tattcggcag tacctccccc ggaacccctg tccgacgccc ccctaccacc accagatgcc
     3181 acccccacac tcggacactg tggaattcta ccagcgcctg tcgaccgaga cactcttctt
     3241 catcttctac tatctggagg gcactaaggc acagtatctg gcagccaagg ccctaaagaa
     3301 gcagtcatgg cgattccaca ccaagtacat gatgtggttc cagaggcacg aggagcccaa
     3361 gaccatcact gacgagtttg agcagggcac ctacatctac tttgactacg agaagtgggg
     3421 ccagcggaag aaggaaggct tcacctttga gtaccgctac ctggaggacc gggacctcca
     3481 gtgcccaact ttcttgtaca aagtggttga tatcggtaag cctatcccta accctctcct
     3541 cggtctcgat tctacgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg
     3601 gctttatata tcttgtggaa aggacgattc gttgtactgg atttcggact cacgcgttaa
     3661 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     3721 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     3781 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     3841 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     3901 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     3961 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     4021 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     4081 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     4141 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     4201 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga
     4261 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     4321 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     4381 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     4441 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     4501 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     4561 gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     4621 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     4681 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     4741 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     4801 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     4861 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     4921 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     4981 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     5041 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     5101 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     5161 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     5221 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     5281 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     5341 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     5401 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     5461 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     5521 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     5581 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     5641 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     5701 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     5761 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     5821 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     5881 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     5941 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     6001 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     6061 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     6121 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     6181 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     6241 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     6301 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     6361 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     6421 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     6481 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     6541 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     6601 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     6661 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     6721 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     6781 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     6841 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     6901 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     6961 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     7021 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     7081 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     7141 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     7201 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     7261 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     7321 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     7381 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     7441 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     7501 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     7561 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     7621 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     7681 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     7741 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     7801 agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
     7861 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
     7921 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
     7981 aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa
     8041 cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     8101 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     8161 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     8221 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     8281 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     8341 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     8401 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     8461 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     8521 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     8581 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     8641 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     8701 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
     8761 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     8821 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     8881 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     8941 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     9001 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     9061 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     9121 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     9181 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     9241 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     9301 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     9361 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     9421 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     9481 accattatcg tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat
     9541 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     9601 atgcgtctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     9661 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     9721 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     9781 ggcctaggct tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta
     9841 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     9901 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     9961 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
    10021 gtgggtcgcg gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc
    10081 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
    10141 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
    10201 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
    10261 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
    10321 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
    10381 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct
    10441 agatcttgag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
    10501 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
    10561 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
    10621 cactttggcg ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag
    10681 gaatctttgc agctaatgga ccttctaggt cttgaaagga gtgggaatt
//
