LOCUS       sequence-34853-e         956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Sun J, Lau A, Wang X, Liao TY, Zoubeidi A, Hmama Z
  TITLE     A broad-range of recombination cloning vectors in mycobacteria.
  JOURNAL   Plasmid. 2009 Nov . 62(3):158-65.
  PUBMED    19647016
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid. 
            2009 Nov . 62(3):158-65."
COMMENT     Sequence Label: pJAK1.B
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(89..95,96..113,114..119))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(144..268)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
       61 ctcgacggat cctaactcaa aatccacaca ttatacgagc cggaagcata aagtgtaaag
      121 cctggggtgc ctaatgcggc cgccatagtg actggatatg ttgtgtttta cagtattatg
      181 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt
      241 ctcgttcagc ttttttgtac aaacttgttg atctggatcc gtactcgacc ggcagccccg
      301 gccgggagaa cgcgcccgcg gttgccgctc cgccggcaag ccccaccagg cccggaagga
      361 ctacagccgc tgccgtgccg atcatcaatc ggcgtcccca agctcgaatc tttcggctca
      421 cgtctgtcat acttgtgccc ctttgtcctg tatgtcgtcg tgtgctcggg ggatccgcaa
      481 ttgtcttggc cattgcgaag tgattcctcc ggatcgggga tgaaacgggg gtcaccgggt
      541 gacggcaacc ccgctactta cgctggccgg gcgcagtgcc cgcgacggac ggctcaagtt
      601 gtcctcgctg ccactcgctg cgacgacggg cctggcctca ccgtcccgac ctagcactca
      661 ccggtcgcga gtgccaacgt tattcttagc actcgcctat gccgagtgca agaagccccg
      721 caccgggtca tgcccctcgt tcgaccttgt cctcggccct ccgatccggg tgagtatgtt
      781 cggcccatga ccgccaacga caacaagacc cgtaaatggt cggccgcaga cgtccccgat
      841 caaagcgggc gcgttgtggt caccgtctag atttctggcg ggaacggcaa tgctcctggc
      901 ggcggcgggg ctggtgccca ggtctccgca cagagcggcg gtgctggcgc tcgcgg
//
