LOCUS       sequence-348531-        1508 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vitro transcription of Patiria miniata Rab7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Swartz SZ, Tan TH, Perillo M, Fakhri N, Wessel GM, Wikramanayake AH,
            Cheeseman IM
  TITLE     Polarized Dishevelled dissolution and reassembly drives embryonic 
            axis specification in sea star oocytes.
  JOURNAL   Curr Biol. 2021 Dec 20;31(24):5633-5641.e4. doi: 
            10.1016/j.cub.2021.10.022. Epub 2021 Nov 4.
  PUBMED    34739818
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2021.10.022"; journalName: "Curr Biol"; date: 
            "2021-12-20- 20"; volume: "31"; issue: "24"; pages: "5633-5641.e4"
COMMENT     Sequence Label: pZS218
FEATURES             Location/Qualifiers
     source          1..1508
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        41..59
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     41..58
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             106..813
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(251..269)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     519..538
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gggaaccgcc attctgcctg gggacgtcgg agcaagcttg atttaggtga cactatagaa
       61 tacaagctac ttgttctttt tgcaggatcc actagtcatc gattgatggt gagcaagggc
      121 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
      181 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
      241 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
      301 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
      361 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
      421 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
      481 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
      541 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
      601 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
      661 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
      721 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
      781 cactccaccg gcggcatgga cgagctgtac aaggaattcg gagccggcgc gccaatgtct
      841 tcaaggaaga aggttttatt gaaggtcatc attctaggag acagtggtgt cggcaagacc
      901 tcgttgatga accaatatgt gaacaagaag tttagtaacc agtacaaggc caccatcggc
      961 gctgacttcc tgacaaaaga ggtcatggta gacgacagac tggtcacaat gcagatatgg
     1021 gacacagccg ggcaggagag gttccagagt ctgggtgtag cgttctaccg cggcgcagac
     1081 tgctgcgtac tcgtctttga cgtgaccaat cagaattcct tcaagtccct agacagctgg
     1141 cgcgatgagt ttctgatcca agccagcccc agagatcctg agaatttccc cttcgtcgta
     1201 ctaggcaaca agatagacat ggagaataga caagtttcga ccaaacgggc gcagaactgg
     1261 tgtggcagca agaacaacat cccttacttt gagaccagcg ctaaggagag tatcaacgtg
     1321 gagcaggcct tccagacgat agccaagaac gccctggccc aggaacccga gacagacatg
     1381 tacaatgatt tccccagcca gatcaaactc accgatgaca ccaaacagga taaatcaggg
     1441 tgttcatgtt aaggccggcc agcgatcgcc aaggcctctc gagcctctag aactatagtg
     1501 agtcgtat
//
