LOCUS       sequence-34854-e         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Sun J, Lau A, Wang X, Liao TY, Zoubeidi A, Hmama Z
  TITLE     A broad-range of recombination cloning vectors in mycobacteria.
  JOURNAL   Plasmid. 2009 Nov . 62(3):158-65.
  PUBMED    19647016
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid. 
            2009 Nov . 62(3):158-65."
COMMENT     Sequence Label: pJAK2.B
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(80..86,87..104,105..110))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(135..259)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgacgga
       61 tcctaactca aaatccacac attatacgag ccggaagcat aaagtgtaaa gcctggggtg
      121 cctaatgcgg ccgccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt
      181 ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt tctcgttcag
      241 cttttttgta caaacttgtt gatctggatc cgtactcgac cggcagcccc ggccgggaga
      301 acgcgcccgc ggttgccgct ccgccggcaa gccccaccag gcccggaagg actacagccg
      361 ctgccgtgcc gatcatcaat cggcgtcccc aagctcgaat ctttcggctc acgtctgtca
      421 tacttgtgcc cctttgtcct gtatgtcgtc gtgtgctcgg gggatccgca attgtcttgg
      481 ccattgcgaa gtgattcctc cggatcgggg atgaaacggg ggtcaccggg tgacggcaac
      541 cccgctactt acgctggccg ggcgcagtgc ccgcgacgga cggctcaagt tgtcctcgct
      601 gccactcgct gcgacgacgg gcctggcctc accgtcccga cctagcactc accggtcgcg
      661 agtgccaacg ttattcttag cactcgccta tgccgagtgc aagaagcccc gcaccgggtc
      721 atgcccctcg ttcgaccttg tcctcggccc tccgatccgg gtgagtatgt tcggcccatg
      781 accgccaacg acaacaagac ccgtaaatgg tcggccgcag acgtccccga tcaaagcggg
      841 cgcgttgtgg tcaccgtcta gatttaaaga tctggtaccg cggccgcgcc acggatgcca
      901 ccacaggcac tacaacggag ttcgccacgt acatcac
//
