LOCUS       sequence-34856-e         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Sun J, Lau A, Wang X, Liao TY, Zoubeidi A, Hmama Z
  TITLE     A broad-range of recombination cloning vectors in mycobacteria.
  JOURNAL   Plasmid. 2009 Nov . 62(3):158-65.
  PUBMED    19647016
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid. 
            2009 Nov . 62(3):158-65."
COMMENT     Sequence Label: pJAK2.D
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(72..78,79..96,97..102))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(127..251)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(264..275)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(276..293)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgacg gatcctaact
       61 caaaatccac acattatacg agccggaagc ataaagtgta aagcctgggg tgcctaatgc
      121 ggccgccata gtgactggat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc
      181 aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc agcttttttg
      241 tacaaacttg ttgatctgga tccgcggccc tcgatgtggt ggtggtggtg gtgcgatccc
      301 gaatggtcga cgctgaaaac tacctcgggc atgtggactc cctttctctt atcgggtggt
      361 ggccgcgagc gcggccggtg gagggttggt gactgacggc gctgcgagac ggcgcccgat
      421 ggggtatgcg agaacaactg cggcaaaacc gatcacggcg atgaccgcgg cggggagcgg
      481 gccggtttcc cagttgcccg acacgatcag gaaggccggc acggcggcgg tgatcacgcc
      541 ttcggccacc gcgacgaacc cgacggcggg ccccagtgcg tcgtgcccca ggccgagcag
      601 caggaacagc atgaaccaca gcactgccca cagcagccag atcaccccga acgccgggtc
      661 ggcctcggcg gtgaacgcgt gccacgagta gccgagtgcg gcgatcgcga cgaacagcga
      721 gaaccatccg agaccttctc cgtcccagtc ggtcacgttg ttgatggcca cccacaggta
      781 ggtgaagccg aacaggtaga gcccggaggc cgcgaagatc acggccgcgt ctccgccgga
      841 ctgcaggatc agcaccg
//
