LOCUS       sequence-348594-        8404 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses red-shifted luciferase under TEF1 promoter in Candida 
            glabrata.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8404)
  AUTHORS   Schrevens S, Sanglard D
  TITLE     Investigating Candida glabrata Urinary Tract Infections (UTIs) in 
            Mice Using Bioluminescence Imaging.
  JOURNAL   J Fungi (Basel). 2021 Oct 9;7(10). pii: jof7100844. doi: 
            10.3390/jof7100844.
  PUBMED    34682265
REFERENCE   2  (bases 1 to 8404)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Fungi 
            (Basel)."; date: "2021-10-9"; volume: "7(10). pii: jof7100844. doi";
            pages: " 10.3390/jof7100844"
COMMENT     Sequence Label: pSSa23
FEATURES             Location/Qualifiers
     source          1..8404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2036..2055)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2037..2055)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2065..2082)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2065..2081)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2074..2096)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2222..2677
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2309..2328)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2519..2540
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5256..5275)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5375..5397
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5435..5453)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6091..6162
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6163..6231,6232..7023)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6381..6400)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7194..7782
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7683..7702
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7936..7953
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8070..8091
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8106..8111,8112..8129,8130..8136)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8144..8160
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8149..8171
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8168..8184
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8168..8184
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8203..8223
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8205..8223
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 atataataca gaccatacag acaatacaga tgggggaccc tctcataatg gccagcatca
       61 acattagtag ttgtaaggag tacccctgta agtgctgctt cctagttggc caccgttgag
      121 agagtacctc ctccctcact tattgctatt attgcttttt attgtttctt ttttcaactg
      181 aaaaattttt ttttttcaat gcgatgagtt tgaagaaaaa atttgaaaaa aattcatcga
      241 tggaataaga tatttaagca gcttgggttt ttaaaacttc tcttgaaaac tttccacttc
      301 ttgttaaatt cctttttgta gaaagttcag cacagataac taacttgaac taatatagca
      361 ggatccaaaa tggaggatgc taagaacatt aagaagggcc ctgccccttt ctatccatta
      421 gaagatggta cagcaggtga acaacttcac aaagctatga agagatacgc tttagtccca
      481 ggtactattg ccttcactga tgcccatata gaggttgaca taacatacgc ggagtacttc
      541 gagatgtcag tgagattagc ggaagccatg aagagatatg gcttgaatac taatcacagg
      601 attgtggttt gctctgaaaa ctcattgcag ttctttatgc cagttttggg tgcattattc
      661 attggtgtcg ctgttgctcc agcaaatgat atatacaacg aaagagaatt attgaactct
      721 atggggatct ctcaaccaac tgtcgtgttt gtcagcaaga agggattgca aaagattctt
      781 aacgttcaga agaaacttcc catcattcag aagatcatca tcatggacag caaaaccgac
      841 tatcaaggct tccaatcaat gtataccttt gttacatctc acctaccacc tgggtttaac
      901 gagtatgact ttgtaccaga gtctttcgat agagataaga ccattgctct gatcatgaat
      961 tcctctggta gtactggtct accaaagggt gttgctctgc cacatagagc attatgtgtc
     1021 agatttagcc atgctagaga tcccatattt ggtaatcaaa ttattccaga cactgccatt
     1081 ctgtccgtcg ttcctttcca ccatggtttt gggatgttca caaccttggg ttacctaatc
     1141 tgtggtttta gagttatgtt gatgtatcgt tttgaagaag agttgttctt gagatcattg
     1201 caagactaca aaatacagac agccctgttg gtcccaacac tgttctcttt cttggctaag
     1261 agtaccctaa tagacaagta cgatttgagt aacctacacg aaatcgcatc tggaggtgca
     1321 ccactttcca aagaggttgg tgaagctgtc gccaaaagat ttcacttacc tggcattaga
     1381 caaggttatg gcctgacaga gactactagt gctatactga ttacccctga aggagatgat
     1441 aaacccggtg cagttggcaa agtggtccca ttctttgaag caaaagttgt agacttagat
     1501 actggcaaaa ctcttggagt gaatcaacgt ggtgagttat gtgtacgtgg tccaatgatt
     1561 atgtccggct acgtcaacaa tccagaagca acaaatgcct taatagacaa agatggatgg
     1621 ctacattccg gtgatatagc ctattgggat gaggacgaac acttcttcat cgtagatagg
     1681 cttaagtctt tgatcaaata caagggttat caagttgctc ctgctgaatt ggagtccatt
     1741 ctactacagc atcctaacat ctttgatgct ggtgttgcag gtttaccaga cgacgatgct
     1801 ggtgaacttc cagcggctgt ggttgtgttg gaacatggta aaaccatgac tgagaaggaa
     1861 atcgttgact acgtagcttc acaagtaact accgcaaaga aactaagagg tggagtagtc
     1921 tttgtagacg aagttccaaa gggtttgaca ggcaaattgg atgccagaaa gattagggaa
     1981 atcttgatta aagctaagaa aggtggcaag attgctgttt aaggtaccca attcgcccta
     2041 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
     2101 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     2161 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     2221 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     2281 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     2341 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     2401 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     2461 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     2521 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     2581 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     2641 aacgcgaatt ttaacaaaat attaacgctt acaatttcct gatgcggtat tttctcctta
     2701 cgcatctgtg cggtatttca caccgcatag ggtaataact gatataatta aattgaagct
     2761 ctaatttgtg agtttagtat acatgcattt acttataata cagtttttta gttttgctgg
     2821 ccgcatcttc tcaaatatgc ttcccagcct gcttttctgt aacgttcacc ctctacctta
     2881 gcatcccttc cctttgcaaa tagtcctctt ccaacaataa taatgtcaga tcctgtagag
     2941 accacatcat ccacggttct atactgttga cccaatgcgt ctcccttgtc atctaaaccc
     3001 acaccgggtg tcataatcaa ccaatcgtaa ccttcatctc ttccacccat gtctctttga
     3061 gcaataaagc cgataacaaa atctttgtcg ctcttcgcaa tgtcaacagt acccttagta
     3121 tattctccag tagataggga gcccttgcat gacaattctg ctaacatcaa aaggcctgtc
     3181 aaaactagag aataataaag aaaacgatct tttcaaaaag aaaaaacctt ttagttttcc
     3241 tttgttgttg ttgtgggtgt gtgctattta tattatatag tttactcata ataccataaa
     3301 atattcggtt tgattaggtt attttaataa gctaatttgt ttctaatcgt gtaatttatg
     3361 ctgtgtatat taagtagtgt gtgcactgcc caaaaatgtt tgttgtttat agtcggttaa
     3421 agagaaaaaa gaaaaaaaga tccatacaca cacgttaatt agttgttcaa cgtaatacac
     3481 tcatattttg ttcttatttg ctttcggtcg ctgttctcac caagatttat tgccaacgaa
     3541 acaatttttt tttatatatt ttcagatttt tctttttttc ctttcctttc cttttctaat
     3601 tttcactcct ggttttcttt ctttcttaga aacattatct cgatattaat attaaaaaaa
     3661 tataatcatt caaaatggac ggtggtatgt tttagtttag cttcaattct aattgattga
     3721 ttaatcagtt gattggtttc aatatgacaa atgggtaggg tgggaaaact tcattttcaa
     3781 ttcagatcaa acttttttgt tgtcgacata atatttctcg tttgggatgt tactgtcaca
     3841 ttaataatac acacacatca gcttataatt ttgaaagtaa tttatcagat atgttgtgac
     3901 gatcagtgga aatggctaac ttcaatgtat ctgttcttcc cctttttcaa agttcacgtt
     3961 ttttgattga ttgattgatc tgtcggcagt ggtttcaaaa ccattcggtg agtaatccta
     4021 tcaatcaatg ttacgacaaa aggctcaata ttcaaaattg caatgtttta tgttttccta
     4081 cgtgtacttg tgcaaggcaa ttgattcaac attgcttttg gtgtttgacg agtttctagt
     4141 ttggacttgt gttgttatct gggctataca gatttcccgg ctcactatga attttttttt
     4201 tcgacgctca gtgcacacaa ctataaacaa cacaaacaca aacacagcaa gaaaaaaaaa
     4261 aaacgaacat tgaattgaaa ccaagccaac tgaaaaattc cttatttaaa tgactgtcat
     4321 actaacccat ttttatagaa gaagttgctg ctttagttat cgataacggt tctcatatga
     4381 aaatttcggt gatccctgag caggtggcgg aaacattgga tgctgagaac catttcattg
     4441 ttcgtgaagt gttcgatgtg cacctatccg accaaggctt tgaactatct accagaagtg
     4501 tgagccccta ccggaaggat tacatctcgg atgatgactc tgatgaagac tctgcttgct
     4561 atggcgcatt catcgaccaa gagcttgtcg ggaagattga actcaactca acatggaacg
     4621 atctagcctc tatcgaacac attgttgtgt cgcacacgca ccgaggcaaa ggagtcgcgc
     4681 acagtctcat cgaatttgcg aaaaagtggg cactaagcag acagctcctt ggcatacgat
     4741 tagagacaca aacgaacaat gtacctgcct gcaatttgta cgcaaaatgt ggctttactc
     4801 tcggcggcat tgacctcttc acgtataaaa ctagacctca agtctcgaac gaaacagcga
     4861 tgtactggta ctggttctcg ggagcacagg atgacgccta acatatgtga agtgtgaagg
     4921 gggagatttt cactttatta gatttgtata tatgtataat aaataaataa ataagttaaa
     4981 taaataatta gataagggtg gtaattatta ctatttacaa tcaaaggtgg tcctgcaggt
     5041 ttttgttctg tgcagttggg ttaagaatac tgggcaattt catgtttctt caacactaca
     5101 tatgcgtata tataccaatc taagtctgtg ctccttcctt cgttcttcct tctgttcgga
     5161 gattaccgaa tcaaaaaaat ttcaaggaaa ccgaaatcaa aaaaaagaat aaaaaaaaaa
     5221 tgatgaattg aaaaggtggt atggtgcact ctcagtacaa tctgctctga tgccgcatag
     5281 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     5341 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     5401 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     5461 gttaatgtca tgataataat ggtttcttag caggttagac taacatgcaa aaagtttata
     5521 ttcatttttg tagttattct tgttttcatt aggatgatta cacactgtgg atgtggatta
     5581 atcgatcatt gttaatttag tgtagctact accatttcaa acaaaacgta aattcttgca
     5641 gtcgtactgg atctgtgaat ctattagtat atatgaatta aagtagcttg tacatattat
     5701 tctgttgaat catatcgcag agcattggtt gaaatccaaa atataaaaat gtaatatcac
     5761 aaaaaataat actaattcta acattaatgg gtcagatttt tagtgaatac ttaaatttat
     5821 aatatcgtct atttaagcta gcaaatggaa caacatttaa agtaagaaca tcatatctac
     5881 atgaaaatgt atatttcaat ctgactaata acgcagagca catctttcag tgatgtctgt
     5941 cacatgatca aaaagaattg tatttaaata attcataata aaagcttaaa aaattacaat
     6001 aatgaaaata aagtaaataa tgacatgggt aagagttccg aaatagaatc ttagtgtaca
     6061 aaacaaaaat tgcatcatta gagatcccca cattcaaata tgtatccgct catgagacaa
     6121 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     6181 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     6241 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     6301 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     6361 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     6421 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     6481 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     6541 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     6601 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     6661 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     6721 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     6781 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     6841 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     6901 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     6961 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     7021 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     7081 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     7141 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     7201 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     7261 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     7321 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     7381 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     7441 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     7501 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     7561 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     7621 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     7681 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     7741 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     7801 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     7861 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     7921 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     7981 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     8041 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     8101 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     8161 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
     8221 agggaacaaa agctggagct cctataccaa catgccaaac cttatgaaga gtcttcaacc
     8281 aaacctacaa tagatgacta cagcgctagc atcataagta gataggtcac caaccaagtg
     8341 atgaagtttg cgttacggca ctggtaacca gctcttgaat tgcttttcca ggaaaagaaa
     8401 cgtc
//
