LOCUS       sequence-348595-        9524 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pGreenFire MPRA plasmid for cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9524)
  AUTHORS   Guo MG, Reynolds DL, Ang CE, Liu Y, Zhao Y, Donohue LKH, Siprashvili
            Z, Yang X, Yoo Y, Mondal S, Hong A, Kain J, Meservey L, Fabo T, 
            Elfaki I, Kellman LN, Abell NS, Pershad Y, Bayat V, Etminani P, 
            Holodniy M, Geschwind DH, Montgomery SB, Duncan LE, Urban AE, Altman
            RB, Wernig M, Khavari PA
  TITLE     Integrative analyses highlight functional regulatory variants 
            associated with neuropsychiatric diseases.
  JOURNAL   Nat Genet. 2023 Nov;55(11):1876-1891. doi: 
            10.1038/s41588-023-01533-5. Epub 2023 Oct 19.
  PUBMED    37857935
REFERENCE   2  (bases 1 to 9524)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41588-023-01533-5"; journalName: "Nat Genet"; date: 
            "2023-11"; volume: "55"; issue: "11"; pages: "1876-1891"
COMMENT     Sequence Label: pGreenFire_blastocidin
FEATURES             Location/Qualifiers
     source          1..9524
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             806..859
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             866..2518
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(932..950)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     misc_feature    2535..3123
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2588..2608)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3006..3017)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        3181..3392
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             3405..3673
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             3700..4098
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             4173..4406
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4478..4599
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4515..4534)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4569..4588
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4639..4774
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4807..4823)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4807..4823)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4820..4842)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4831..4847
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4855..4861,4862..4879,4880..4885))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4900..4921
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5038..5055)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5209..5797)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5289..5308)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5968..6759,6760..6828))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6591..6610
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6829..6933)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7001..7019
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     7179..7198
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7392..7414
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7406..7423
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7407..7423
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7438..7664
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7665..7845
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7892..8017
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8510..8743
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8928..8972
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9239..9355
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     9411..9435
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(9523..9524,1..664)
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="MPAMKIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGAL
                     TFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYR
                     YEAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDG
                     GYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA
                     "
ORIGIN
        1 gcccgccatg aagatcgagt gccgcatcac cggcaccctg aacggcgtgg agttcgagct
       61 ggtgggcggc ggagagggca cccccgagca gggccgcatg accaacaaga tgaagagcac
      121 caaaggcgcc ctgaccttca gcccctacct gctgagccac gtgatgggct acggcttcta
      181 ccacttcggc acctacccca gcggctacga gaaccccttc ctgcacgcca tcaacaacgg
      241 cggctacacc aacacccgca tcgagaagta cgaggacggc ggcgtgctgc acgtgagctt
      301 cagctaccgc tacgaggccg gccgcgtgat cggcgacttc aaggtggtgg gcaccggctt
      361 ccccgaggac agcgtgatct tcaccgacaa gatcatccgc agcaacgcca ccgtggagca
      421 cctgcacccc atgggcgata acgtgctggt gggcagcttc gcccgcacct tcagcctgcg
      481 cgacggcggc tactacagct tcgtggtgga cagccacatg cacttcaaga gcgccatcca
      541 ccccagcatc ctgcagaacg ggggccccat gttcgccttc cgccgcgtgg aggagctgca
      601 cagcaacacc gagctgggca tcgtggagta ccagcacgcc ttcaagaccc ccatcgcctt
      661 cgccagatct cgagatatca gccatggctt cccgccggcg gtggcggcgc aggatgatgg
      721 cacgctgccc atgtcttgtg cccaggagag cgggatggac cgtcaccctg cagcctgtgc
      781 ttctgctagg atcaatgtga ccggtgaggg cagaggaagt cttctaacat gcggtgacgt
      841 ggaggagaat cccggccctt ccggtatgga agacgccaaa aacataaaga aaggcccggc
      901 gccattctat ccactagagg atggaaccgc tggagagcaa ctgcataagg ctatgaagag
      961 atacgccctg gttcctggaa caattgcttt tacagatgca catatcgagg tgaacatcac
     1021 gtacgcggaa tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac gatatgggct
     1081 gaatacaaat cacagaatcg tcgtatgcag tgaaaactct cttcaattct ttatgccggt
     1141 gttgggcgcg ttatttatcg gagttgcagt tgcgcccgcg aacgacattt ataatgaacg
     1201 tgaattgctc aacagtatga acatttcgca gcctaccgta gtgtttgttt ccaaaaaggg
     1261 gttgcaaaaa attttgaacg tgcaaaaaaa attaccaata atccagaaaa ttattatcat
     1321 ggattctaaa acggattacc agggatttca gtcgatgtac acgttcgtca catctcatct
     1381 acctcccggt tttaatgaat acgattttgt accagagtcc tttgatcgtg acaaaacaat
     1441 tgcactgata atgaactcct ctggatctac tgggttacct aagggtgtgg cccttccgca
     1501 tagaactgcc tgcgtcagat tctcgcatgc cagagatcct atttttggca atcaaatcat
     1561 tccggatact gcgattttaa gtgttgttcc attccatcac ggttttggaa tgtttactac
     1621 actcggatat ttgatatgtg gatttcgagt cgtcttaatg tatagatttg aagaagagct
     1681 gtttttacga tcccttcagg attacaaaat tcaaagtgcg ttgctagtac caaccctatt
     1741 ttcattcttc gccaaaagca ctctgattga caaatacgat ttatctaatt tacacgaaat
     1801 tgcttctggg ggcgcacctc tttcgaaaga agtcggggag gcggttgcaa aacgcttcca
     1861 tcttccaggg atacgacaag gatatgggct cactgagact acatcagcta ttctgattac
     1921 acccgagggg gatgataaac cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa
     1981 ggttgtggat ctggataccg ggaaaacgct gggcgttaat cagagaggcg aattatgtgt
     2041 cagaggacct atgattatgt ccggttatgt aaacaatccg gaagcgacca acgccttgat
     2101 tgacaaggat ggatggctac attctggaga catagcttac tgggacgaag acgaacactt
     2161 cttcatagtt gaccgcttga agtctttaat taaatacaaa ggataccagg tggcccccgc
     2221 tgaattggag tcgatattgt tacaacaccc caacatcttc gacgcgggcg tggcaggtct
     2281 tcccgacgat gacgccggtg aacttcccgc cgccgttgtt gttttggagc acggaaagac
     2341 gatgacggaa aaagagatcg tggattacgt cgccagtcaa gtaacaaccg cgaaaaagtt
     2401 gcgcggagga gttgtgtttg tggacgaagt accgaaaggt cttaccggaa aactcgacgc
     2461 aagaaaaatc agagagatcc tcataaaggc caagaagggc ggaaagtcca aattgtaagg
     2521 atcctcgact cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     2581 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     2641 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     2701 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     2761 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     2821 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     2881 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     2941 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     3001 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     3061 ctcttccgca tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     3121 cgcctggaat taattcgagc tcggtaccaa ggatctgcga tcgctccggt gcccgtcagt
     3181 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     3241 cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     3301 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     3361 tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct cgcatctctc
     3421 cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt cgcgttctgc
     3481 cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca
     3541 ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc agccggctct
     3601 ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt tctgttctgc
     3661 gccgttacag atccaagctg tgaccggcgc ctacgctaga tggccaagcc tttgtctcaa
     3721 gaagaatcca ccctcattga aagagcaacg gctacaatca acagcatccc catctctgaa
     3781 gactacagcg tcgccagcgc agctctctct agcgacggcc gcatcttcac tggtgtcaat
     3841 gtatatcatt ttactggggg accttgtgca gaactcgtgg tgctgggcac tgctgctgct
     3901 gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg
     3961 agcccctgcg gacggtgccg acaggtgctt ctcgatctgc atcctgggat caaagccata
     4021 gtgaaggaca gtgatggaca gccgacggca gttgggattc gtgaattgct gccctctggt
     4081 tatgtgtggg agggctaatg aggtaccttt aagaccaatg acttacaagg cagctgtaga
     4141 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     4201 ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     4261 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     4321 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     4381 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     4441 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     4501 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     4561 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     4621 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     4681 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     4741 ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga ccaaattcgt
     4801 aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     4861 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     4921 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     4981 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     5041 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     5101 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     5161 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     5221 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     5281 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     5341 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     5401 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     5461 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     5521 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     5581 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     5641 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     5701 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     5761 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     5821 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     5881 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     5941 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     6001 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     6061 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     6121 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     6181 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     6241 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     6301 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     6361 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     6421 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     6481 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     6541 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     6601 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     6661 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     6721 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     6781 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     6841 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     6901 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     6961 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     7021 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     7081 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     7141 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
     7201 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     7261 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     7321 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     7381 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tgacgcgtgt
     7441 agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct
     7501 tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg
     7561 ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc
     7621 attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag
     7681 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     7741 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     7801 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     7861 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     7921 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     7981 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     8041 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     8101 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     8161 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     8221 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     8281 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     8341 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     8401 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     8461 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     8521 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     8581 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     8641 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     8701 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     8761 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     8821 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     8881 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     8941 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     9001 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     9061 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     9121 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     9181 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt
     9241 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     9301 caacagacat acaaactaaa gaattacaaa aacaaattac aaaattcaaa attttatcga
     9361 tgaattctag aactagttag gcgtgtacgg tgggaggcct atataagcag agctcgttta
     9421 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
     9481 cgggaccgat ccagcctctc gacattcgtt ggatccgcca ccat
//
