LOCUS       sequence-348596-        8572 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses red-shifted luciferase under ENO1 promoter in Candida 
            glabrata.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8572)
  AUTHORS   Schrevens S, Sanglard D
  TITLE     Investigating Candida glabrata Urinary Tract Infections (UTIs) in 
            Mice Using Bioluminescence Imaging.
  JOURNAL   J Fungi (Basel). 2021 Oct 9;7(10). pii: jof7100844. doi: 
            10.3390/jof7100844.
  PUBMED    34682265
REFERENCE   2  (bases 1 to 8572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Fungi 
            (Basel)."; date: "2021-10-9"; volume: "7(10). pii: jof7100844. doi";
            pages: " 10.3390/jof7100844"
COMMENT     Sequence Label: pSSa16
FEATURES             Location/Qualifiers
     source          1..8572
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2395..2414)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2396..2414)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2424..2441)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2424..2440)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2433..2455)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2581..3036
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2668..2687)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2878..2899
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5615..5634)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5734..5756
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5794..5812)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6450..6521
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6522..6590,6591..7382)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6740..6759)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7553..8141
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8042..8061
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8295..8312
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8429..8450
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8465..8470,8471..8488,8489..8495)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8503..8519
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8508..8530
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8527..8543
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8527..8543
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     join(8562..8572,1..10)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        join(8564..8572,1..10)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tcactaaagg gaacaaaagc tggagctccc gcgggtttcg aaacaaagca gagtgtacac
       61 ccatacatcc cgacatccac taccttaata gtactgttca attttcacta cacaggttac
      121 gccagtcgca caagttgcac tgtacaatcc tgcgggccct ctgacagtgg cattccagtt
      181 agatcggaag ggagaggaac aatggggggg ggggaggaca atgggattcc actagcaact
      241 gcgaaaactg caagagctag tggaatcccc gcgaccggta actggaacgc agaggggccc
      301 aatatatggt gttaaagtgt ggcgagagtc tctctgccat aacatgcacc cgtccaccac
      361 acacataatt ccatctaacc cagttcccat ttagcccttc cagcgattcc agcgattcca
      421 gcgattccag cgattccact gattcccaca taaagcacgc gcaacacaaa gggagggttg
      481 cgacccaacg tgtgatgtca gtgtggaagc gatggcctta tgatggcctt taggcccaca
      541 agaaagaaaa aaaaatgaaa ttcgaggaaa tttttttgct atataaggag agcaattggg
      601 aaattgaatc acttgtttag atcgtctggt ttttccttgt taggagttga agaaaaaggg
      661 taaggtagaa atattatata tttatactct caacaacaaa acacatatta taaatcatag
      721 gatccaaaat ggaggatgct aagaacatta agaagggccc tgcccctttc tatccattag
      781 aagatggtac agcaggtgaa caacttcaca aagctatgaa gagatacgct ttagtcccag
      841 gtactattgc cttcactgat gcccatatag aggttgacat aacatacgcg gagtacttcg
      901 agatgtcagt gagattagcg gaagccatga agagatatgg cttgaatact aatcacagga
      961 ttgtggtttg ctctgaaaac tcattgcagt tctttatgcc agttttgggt gcattattca
     1021 ttggtgtcgc tgttgctcca gcaaatgata tatacaacga aagagaatta ttgaactcta
     1081 tggggatctc tcaaccaact gtcgtgtttg tcagcaagaa gggattgcaa aagattctta
     1141 acgttcagaa gaaacttccc atcattcaga agatcatcat catggacagc aaaaccgact
     1201 atcaaggctt ccaatcaatg tatacctttg ttacatctca cctaccacct gggtttaacg
     1261 agtatgactt tgtaccagag tctttcgata gagataagac cattgctctg atcatgaatt
     1321 cctctggtag tactggtcta ccaaagggtg ttgctctgcc acatagagca ttatgtgtca
     1381 gatttagcca tgctagagat cccatatttg gtaatcaaat tattccagac actgccattc
     1441 agtccgtcgt tcctttccac catggttttg ggatgttcac aaccttgggt tacctaatct
     1501 gtggttttag agttatgttg atgtatcgtt ttgaagaaga gttgttcttg agatcattgc
     1561 aagactacaa aatacagaca gccctgttgg tcccaacact gttctctttc ttggctaaga
     1621 gtaccctaat agacaagtac gatttgagta acctacacga aatcgcatct ggaggtgcac
     1681 cactttccaa agaggttggt gaagctgtcg ccaaaagatt tcacttacct ggcattagac
     1741 aaggttatgg cctgacagag actactagtg ctatactgat tacccctgaa ggagatgata
     1801 aacccggtgc agttggcaaa gtggtcccat tctttgaagc aaaagttgta gacttagata
     1861 ctggcaaaac tcttggagtg aatcaacgtg gtgagttatg tgtacgtggt ccaatgatta
     1921 tgtccggcta cgtcaacaat ccagaagcaa caaatgcctt aatagacaaa gatggatggc
     1981 tacattccgg tgatatagcc tattgggatg aggacgaaca cttcttcatc gtagataggc
     2041 ttaagtcttt gatcaaatac aagggttatc aagttgctcc tgctgaattg gagtccattc
     2101 tactacagca tcctaacatc tttgatgctg gtgttgcagg tttaccagac gacgatgctg
     2161 gtgaacttcc agcggctgtg gttgtgttgg aacatggtaa aaccatgact gagaaggaaa
     2221 tcgttgacta cgtagcttca caagtaacta ccgcaaagaa actaagaggt ggagtagtct
     2281 ttgtagacga agttccaaag ggtttgacag gcaaattgga tgccagaaag attagggaaa
     2341 tcttgattaa agctaagaaa ggtggcaaga ttgctgttta aggtacccaa ttcgccctat
     2401 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     2461 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     2521 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     2581 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     2641 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     2701 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     2761 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     2821 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     2881 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     2941 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     3001 acgcgaattt taacaaaata ttaacgctta caatttcctg atgcggtatt ttctccttac
     3061 gcatctgtgc ggtatttcac accgcatagg gtaataactg atataattaa attgaagctc
     3121 taatttgtga gtttagtata catgcattta cttataatac agttttttag ttttgctggc
     3181 cgcatcttct caaatatgct tcccagcctg cttttctgta acgttcaccc tctaccttag
     3241 catcccttcc ctttgcaaat agtcctcttc caacaataat aatgtcagat cctgtagaga
     3301 ccacatcatc cacggttcta tactgttgac ccaatgcgtc tcccttgtca tctaaaccca
     3361 caccgggtgt cataatcaac caatcgtaac cttcatctct tccacccatg tctctttgag
     3421 caataaagcc gataacaaaa tctttgtcgc tcttcgcaat gtcaacagta cccttagtat
     3481 attctccagt agatagggag cccttgcatg acaattctgc taacatcaaa aggcctgtca
     3541 aaactagaga ataataaaga aaacgatctt ttcaaaaaga aaaaaccttt tagttttcct
     3601 ttgttgttgt tgtgggtgtg tgctatttat attatatagt ttactcataa taccataaaa
     3661 tattcggttt gattaggtta ttttaataag ctaatttgtt tctaatcgtg taatttatgc
     3721 tgtgtatatt aagtagtgtg tgcactgccc aaaaatgttt gttgtttata gtcggttaaa
     3781 gagaaaaaag aaaaaaagat ccatacacac acgttaatta gttgttcaac gtaatacact
     3841 catattttgt tcttatttgc tttcggtcgc tgttctcacc aagatttatt gccaacgaaa
     3901 caattttttt ttatatattt tcagattttt ctttttttcc tttcctttcc ttttctaatt
     3961 ttcactcctg gttttctttc tttcttagaa acattatctc gatattaata ttaaaaaaat
     4021 ataatcattc aaaatggacg gtggtatgtt ttagtttagc ttcaattcta attgattgat
     4081 taatcagttg attggtttca atatgacaaa tgggtagggt gggaaaactt cattttcaat
     4141 tcagatcaaa cttttttgtt gtcgacataa tatttctcgt ttgggatgtt actgtcacat
     4201 taataataca cacacatcag cttataattt tgaaagtaat ttatcagata tgttgtgacg
     4261 atcagtggaa atggctaact tcaatgtatc tgttcttccc ctttttcaaa gttcacgttt
     4321 tttgattgat tgattgatct gtcggcagtg gtttcaaaac cattcggtga gtaatcctat
     4381 caatcaatgt tacgacaaaa ggctcaatat tcaaaattgc aatgttttat gttttcctac
     4441 gtgtacttgt gcaaggcaat tgattcaaca ttgcttttgg tgtttgacga gtttctagtt
     4501 tggacttgtg ttgttatctg ggctatacag atttcccggc tcactatgaa tttttttttt
     4561 cgacgctcag tgcacacaac tataaacaac acaaacacaa acacagcaag aaaaaaaaaa
     4621 aacgaacatt gaattgaaac caagccaact gaaaaattcc ttatttaaat gactgtcata
     4681 ctaacccatt tttatagaag aagttgctgc tttagttatc gataacggtt ctcatatgaa
     4741 aatttcggtg atccctgagc aggtggcgga aacattggat gctgagaacc atttcattgt
     4801 tcgtgaagtg ttcgatgtgc acctatccga ccaaggcttt gaactatcta ccagaagtgt
     4861 gagcccctac cggaaggatt acatctcgga tgatgactct gatgaagact ctgcttgcta
     4921 tggcgcattc atcgaccaag agcttgtcgg gaagattgaa ctcaactcaa catggaacga
     4981 tctagcctct atcgaacaca ttgttgtgtc gcacacgcac cgaggcaaag gagtcgcgca
     5041 cagtctcatc gaatttgcga aaaagtgggc actaagcaga cagctccttg gcatacgatt
     5101 agagacacaa acgaacaatg tacctgcctg caatttgtac gcaaaatgtg gctttactct
     5161 cggcggcatt gacctcttca cgtataaaac tagacctcaa gtctcgaacg aaacagcgat
     5221 gtactggtac tggttctcgg gagcacagga tgacgcctaa catatgtgaa gtgtgaaggg
     5281 ggagattttc actttattag atttgtatat atgtataata aataaataaa taagttaaat
     5341 aaataattag ataagggtgg taattattac tatttacaat caaaggtggt cctgcaggtt
     5401 tttgttctgt gcagttgggt taagaatact gggcaatttc atgtttcttc aacactacat
     5461 atgcgtatat ataccaatct aagtctgtgc tccttccttc gttcttcctt ctgttcggag
     5521 attaccgaat caaaaaaatt tcaaggaaac cgaaatcaaa aaaaagaata aaaaaaaaat
     5581 gatgaattga aaaggtggta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     5641 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     5701 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     5761 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     5821 ttaatgtcat gataataatg gtttcttagc aggttagact aacatgcaaa aagtttatat
     5881 tcatttttgt agttattctt gttttcatta ggatgattac acactgtgga tgtggattaa
     5941 tcgatcattg ttaatttagt gtagctacta ccatttcaaa caaaacgtaa attcttgcag
     6001 tcgtactgga tctgtgaatc tattagtata tatgaattaa agtagcttgt acatattatt
     6061 ctgttgaatc atatcgcaga gcattggttg aaatccaaaa tataaaaatg taatatcaca
     6121 aaaaataata ctaattctaa cattaatggg tcagattttt agtgaatact taaatttata
     6181 atatcgtcta tttaagctag caaatggaac aacatttaaa gtaagaacat catatctaca
     6241 tgaaaatgta tatttcaatc tgactaataa cgcagagcac atctttcagt gatgtctgtc
     6301 acatgatcaa aaagaattgt atttaaataa ttcataataa aagcttaaaa aattacaata
     6361 atgaaaataa agtaaataat gacatgggta agagttccga aatagaatct tagtgtacaa
     6421 aacaaaaatt gcatcattag agatcccaac attcaaatat gtatccgctc atgagacaat
     6481 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     6541 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     6601 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     6661 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     6721 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     6781 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     6841 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     6901 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     6961 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     7021 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     7081 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     7141 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     7201 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     7261 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     7321 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     7381 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     7441 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     7501 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     7561 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     7621 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     7681 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     7741 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     7801 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     7861 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     7921 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     7981 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     8041 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     8101 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     8161 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     8221 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     8281 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     8341 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     8401 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     8461 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     8521 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cc
//
