LOCUS       sequence-348599-        6839 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Aequorin in Candida albicans using the URA3 auxotrophic 
            marker URA3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6839)
  AUTHORS   Sanglard D
  TITLE     Aequorin as a Useful Calcium-Sensing Reporter in Candida albicans.
  JOURNAL   J Fungi (Basel). 2021 Apr 20;7(4). pii: jof7040319. doi: 
            10.3390/jof7040319.
  PUBMED    33924126
REFERENCE   2  (bases 1 to 6839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Fungi 
            (Basel)."; date: "2021-04-20"; volume: "7(4). pii: jof7040319. doi";
            pages: " 10.3390/jof7040319"
COMMENT     Sequence Label: pDS1209
FEATURES             Location/Qualifiers
     source          1..6839
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    121..142
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(157..162,163..180,181..187)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    195..211
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     200..222
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     219..235
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     219..235
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        256..274
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2852..2868)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2852..2868)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(4283..4302)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4284..4302)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4309..4326)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4309..4325)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4318..4340)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(4466..4921)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4603..4624)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4815..4834
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4948..5052
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5053..5121,5122..5913)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5271..5290)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6084..6672
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6573..6592
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(6826..6839,1..4)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
       61 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
      121 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
      181 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      241 ttacgccaag ctcgaaatta accctcacta aagggaacaa aagctggtac cgggcccccc
      301 ctcgagctat taagatcacc agcctcgttt ataataaact tagtctaata tttcttttgg
      361 accaaaatag aaactgttct caggtggcaa aaaacagctt aaagtctgtg ttaaaaaaag
      421 atctcggcta tgccaatcaa aaaggagtga cattattatg taaaagaaga aatgttgtaa
      481 ttccgcgaaa caacaatttt aatgcttact gtgccgccac caatatccta tggccaaggg
      541 gcctttaaac atgccacgga aatatacagt caaagtaaac ctactatgtc tttagagcct
      601 tcaggatcaa ttgtttttaa atgaatgact gcaaaactag aaaagtagcc tcttacataa
      661 gcaagtcatt tgtacaccga gagtttagta accagtgaag caagtatatc tggcaaacct
      721 gaaacttatc tgtgaattat aaagctcata agtaaaaagt gtagaaaaga agataaggca
      781 gaccaaatac aaatttgcaa cagtacaacg aatatttcag cgtcaaaact agagaataat
      841 aaagaaaacg atcttttcaa aaagaaaaaa ccttttagtt ttcctttgtt gttgttgtgg
      901 gtgtgtgcta tttatattat atagtttact cataatacca taaaatattc ggtttgatta
      961 ggttatttta ataagctaat ttgtttctaa tcgtgtaatt tatgctgtgt atattaagta
     1021 gtgtgtgcac tgcccaaaaa tgtttgttgt ttatagtcgg ttaaagagaa aaaagaaaaa
     1081 aagatccata cacacacgtt aattagttgt tcaacgtaat acactcatat tttgttctta
     1141 tttgctttcg gtcgctgttc tcaccaagat ttattgccaa cgaaacaatt tttttttata
     1201 tattttcaga tttttctttt tttcctttcc tttccttttc taattttcac tcctggtttt
     1261 ctttctttct tagaaacatt atctcgatat taatattaaa aaaatataat cattcaaact
     1321 gcagaaaatg acaagcaaac aatactcagt caagcttaca ccagacttcg acaacccaaa
     1381 atggattgga cgacacaagc acatgtttaa ttttcttgat gtcaaccaca atggaaggat
     1441 ctctcttgac gagatggtct acaaggcgtc cgatattgtt ataaacaatc ttggagcaac
     1501 acctgaacaa gccaaacgtc acaaagatgc tgtagaagcc ttcttcggag gagctggaat
     1561 gaaatacggt gtagaaactg aatggcctga atacatcgaa ggatggaaaa gattggcttc
     1621 cgaggaattg aaaaggtatt caaaaaacca aatcacactt attcgtttat ggggtgatgc
     1681 attgttcgat atcattgaca aagatcaaaa tggagccatt acattggatg aatggaaagc
     1741 atacaccaaa tctgctggca tcatccaatc gtcagaagat tgcgaggaaa cattcagagt
     1801 gtgcgatatt gatgaaagtg gacagctcga tgttgatgag atgacaagac aacatttagg
     1861 attttggtac accatggatc ctgcttgcga aaagctctac ggtggagctg tcccctaagc
     1921 tagcgtccct atttattttt ttatagttat gttagtatta agaacgttat ttatatttca
     1981 aattcgcgta gatccaactc aagtacaaca tggctgtcgg taaaaacaag agattgtcca
     2041 aaggaaagaa aggattaaaa aagaaggtcg ttgacccatt caccagaaaa gattggtttg
     2101 acatcaaagc tccaaccact tttgaaaaca gaaatgttgg taaaactttg atcaacagat
     2161 ctaccggttt aaagaatgcc gctgatggct tgaaaggtag agttttcgaa gtttgtttgg
     2221 ccgacttaca aggttccgaa gaccactctt acagaaaaat caaattgaga gttgatgaag
     2281 tccaaggtaa aaacttgttg accaacttcc atgaggcctc catggaggcc tcatggaggc
     2341 ctcatggagg cctcatggag gcctcatggt ttggatttca cttctgacaa attaagatca
     2401 ttggtcagaa aatggcaatc attagttgaa gctaatgtca ctgttaaaac ttccgacgat
     2461 tacgttttga gagtttttgc cattgctttc accaaaagac aaccaaacca aatcaagaaa
     2521 actacttacg ctcaatcctc taaattgaga gaagtcagaa agaagatgat tgaaattatg
     2581 caaagagaag tttccaactg tactttagct caattaactt ccaaattgat tccagaagtc
     2641 attggccgtg aaattgaaaa atccacccaa accattttcc cattacaaaa tgtccacatc
     2701 agaaaagtca aattgttgaa acaaccaaaa ttcgacttgg gttcattatt ggctttgcac
     2761 ggtgaaggtt caaccgaaga aaaaggtaag aaagtttctt ctggtttcaa agatgttgtt
     2821 ttagaatctg tttaaggata aggatctggg ggatccacta gttctagaag gaccaccttt
     2881 gattgtaaat agtaataatt accaccctta tttaattatt tatttaactt atttatttat
     2941 ttattataca tatatacaaa tctaataaag tgaaaatctc ccccttcaca tttataattg
     3001 gccagttttt ttcaaataag cattccaacc agcatctcta taccttttac cttcaatatc
     3061 tggatctctt cctttaccaa acaatcctct accaacaatg ataatatcag ttccagtgct
     3121 aacaacttca tcaacagttc tatattgttg tcctaatcca tcacctttat catctaatcc
     3181 aactccaggt gtcataataa gccaatcaaa tccttcttct tgtccaccca tatcacgttg
     3241 ggcaataaat ccaataacaa attccttatc ggatttagca atttcaacag ttttttgaga
     3301 atattctcca tatgctaatg atcccactga tgataattca gctaacatca ataaccctct
     3361 tggctcttgg ttggtggtgg tttctttagc tccctgtttt aatccttcaa ctactccatt
     3421 cccagtgaca ccatgagcat tagtaatatc tgcccaacta ctaattttat aaactccacc
     3481 aatatattgt ttcttcacgg tattaccaat atcagcaaat tttctatctt caaaaatcat
     3541 aaattgatgt ttacgtgaaa gttctaataa tggttcaata gtggattcat aggaaaaatc
     3601 attgattata tcaatatgag tcttgattaa gcatacataa ggacccaatt tatcaattaa
     3661 ttcaaggaat tccttagtgg tatcaacatc aattgatgca cataaattgg ttttcttcag
     3721 ttccattaat cgaaataatc gttgtgctac tggtgaggca tgagtttctg ctctctcact
     3781 ataggtctta gtgttgactg tcatatcttt tgtttgctat ttctatttct atttctttct
     3841 tcaactgtaa aaggggcgat atataaacca gtgtgtatgg ggttgttgct caggaattga
     3901 tcaatgttta aaagaatggt tgaaatcatc attgaagaac aaaaaaaaag attttttctt
     3961 tcattatgtt atctgtataa tatgcaggaa ataagattgc aaaaaaatag ccgttttttt
     4021 ggatactatc aaacaagagg aatgactaat acagaattag tgttacgaat caatggcact
     4081 acagcaactt tcaacaaaat aatctacgtt caaaatataa tatatactat taaataaaat
     4141 caaataataa aataatttta aagaaagtag taccagctct tttttttgtt tccgtttata
     4201 ccatccaaat caattcctat tagtcgggat ctaggcctgg ccaagtcggc cgcggccgcc
     4261 accgcggtgg agctccaatt cgccctatag tgagtcgtat tacaattcac tggccgtcgt
     4321 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     4381 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     4441 gttgcgcagc ctgaatggcg aatggaaatt gtaagcgtta atattttgtt aaaattcgcg
     4501 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     4561 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     4621 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     4681 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     4741 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     4801 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     4861 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     4921 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     4981 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     5041 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     5101 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     5161 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     5221 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     5281 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     5341 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     5401 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     5461 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     5521 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     5581 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     5641 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     5701 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     5761 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     5821 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     5881 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     5941 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     6001 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     6061 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     6121 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     6181 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     6241 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     6301 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     6361 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     6421 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     6481 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     6541 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     6601 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     6661 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     6721 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     6781 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgag
//
