LOCUS       sequence-348600-        7316 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Aequorin in Candida albicans using the dominant marker 
            SAT1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7316)
  AUTHORS   Sanglard D
  TITLE     Aequorin as a Useful Calcium-Sensing Reporter in Candida albicans.
  JOURNAL   J Fungi (Basel). 2021 Apr 20;7(4). pii: jof7040319. doi: 
            10.3390/jof7040319.
  PUBMED    33924126
REFERENCE   2  (bases 1 to 7316)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Fungi 
            (Basel)."; date: "2021-04-20"; volume: "7(4). pii: jof7040319. doi";
            pages: " 10.3390/jof7040319"
COMMENT     Sequence Label: pDS1885
FEATURES             Location/Qualifiers
     source          1..7316
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3861..3880)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3862..3880)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3887..3904)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3887..3903)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3896..3918)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(4044..4499)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4181..4202)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4393..4412
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4526..4630
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4631..4699,4700..5491)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4849..4868)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5662..6250
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6151..6170
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6404..6421
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6538..6559
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6574..6579,6580..6597,6598..6604)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6612..6628
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6617..6639
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6636..6652
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6636..6652
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        6673..6691
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggtgtgtgct atttatatta tatagtttac tcataatacc ataaaatatt cggtttgatt
       61 aggttatttt aataagctaa tttgtttcta atcgtgtaat ttatgctgtg tatattaagt
      121 agtgtgtgca ctgcccaaaa atgtttgttg tttatagtcg gttaaagaga aaaaagaaaa
      181 aaagatccat acacacacgt taattagttg ttcaacgtaa tacactcata ttttgttctt
      241 atttgctttc ggtcgctgtt ctcaccaaga tttattgcca acgaaacaat ttttttttat
      301 atattttcag atttttcttt ttttcctttc ctttcctttt ctaattttca ctcctggttt
      361 tctttctttc ttagaaacat tatctcgata ttaatattaa aaaaatataa tcattcaaac
      421 tgcagaaaat gacaagcaaa caatactcag tcaagcttac accagacttc gacaacccaa
      481 aatggattgg acgacacaag cacatgttta attttcttga tgtcaaccac aatggaagga
      541 tctctcttga cgagatggtc tacaaggcgt ccgatattgt tataaacaat cttggagcaa
      601 cacctgaaca agccaaacgt cacaaagatg ctgtagaagc cttcttcgga ggagctggaa
      661 tgaaatacgg tgtagaaact gaatggcctg aatacatcga aggatggaaa agattggctt
      721 ccgaggaatt gaaaaggtat tcaaaaaacc aaatcacact tattcgttta tggggtgatg
      781 cattgttcga tatcattgac aaagatcaaa atggagccat tacattggat gaatggaaag
      841 catacaccaa atctgctggc atcatccaat cgtcagaaga ttgcgaggaa acattcagag
      901 tgtgcgatat tgatgaaagt ggacagctcg atgttgatga gatgacaaga caacatttag
      961 gattttggta caccatggat cctgcttgcg aaaagctcta cggtggagct gtcccctaag
     1021 ctagcgtccc tatttatttt tttatagtta tgttagtatt aagaacgtta tttatatttc
     1081 aaattcgcgt agatccaact caagtacaac atggctgtcg gtaaaaacaa gagattgtcc
     1141 aaaggaaaga aaggattaaa aaagaaggtc gttgacccat tcaccagaaa agattggttt
     1201 gacatcaaag ctccaaccac ttttgaaaac agaaatgttg gtaaaacttt gatcaacaga
     1261 tctaccggtt taaagaatgc cgctgatggc ttgaaaggta gagttttcga agtttgtttg
     1321 gccgacttac aaggttccga agaccactct tacagaaaaa tcaaattgag agttgatgaa
     1381 gtccaaggta aaaacttgtt gaccaacttc catgaggcct ccatggaggc ctcatggagg
     1441 cctcatggag gcctcatgga ggcctcatgg tttggatttc acttctgaca aattaagatc
     1501 attggtcaga aaatggcaat cattagttga agctaatgtc actgttaaaa cttccgacga
     1561 ttacgttttg agagtttttg ccattgcttt caccaaaaga caaccaaacc aaatcaagaa
     1621 aactacttac gctcaatcct ctaaattgag agaagtcaga aagaagatga ttgaaattat
     1681 gcaaagagaa gtttccaact gtactttagc tcaattaact tccaaattga ttccagaagt
     1741 cattggccgt gaaattgaaa aatccaccca aaccattttc ccattacaaa atgtccacat
     1801 cagaaaagtc aaattgttga aacaaccaaa attcgacttg ggttcattat tggctttgca
     1861 cggtgaaggt tcaaccgaag aaaaaggtaa gaaagtttct tctggtttca aagatgttgt
     1921 tttagaatct gtttaaggat aaggatctgg gggatccact agttctagtt ctagaggacc
     1981 acctttgatt gtaaatagta ataattacca cccttatcta attatttatt taacttattt
     2041 atttatttat tatacatata tacaaatcta ataaagtgaa aatctccccc ttcacacttc
     2101 acatatgtta ggcgtcatcc tgtgctcccg agaaccagta ccagtacatc gctgtttcgt
     2161 tcgagacttg aggtctagtt ttatacgtga agaggtcaat gccgccgaga gtaaagccac
     2221 attttgcgta caaattgcag gcaggtacat tgttcgtttg tgtctctaat cgtatgccaa
     2281 ggagctgtct gcttagtgcc cactttttcg caaattcgat gagactgtgc gcgactcctt
     2341 tgcctcggtg cgtgtgcgac acaacaatgt gttcgataga ggctagatcg ttccatgttg
     2401 agttgagttc aatcttcccg acaagctctt ggtcgatgaa tgcgccatag caagcagagt
     2461 cttcatcaga gtcatcatcc gagatgtaat ccttccggta ggggctcaca cttctggtag
     2521 atagttcaaa gccttggtcg gataggtgca catcgaacac ttcacgaaca atgaaatggt
     2581 tctcagcatc caatgtttcc gccacctgct cagggatcac cgaaattttc atatgagaac
     2641 cgttatcgat aactaaagca gcaacttctt ctataaaaat gggttagtat gacagtcatt
     2701 taaataagga atttttcagt tggcttggtt tcaattcaat gttcgttttt tttttcttgc
     2761 tgtgtttgtg tttgtgttgt ctatagttgt gtgcactgag cgtcgaaaaa aaaaattcat
     2821 agtgagccgg gaaatctgta tagcccagat aacaacacaa gtccaaacta gaaactcgtc
     2881 aaacaccaaa agcaatgttg aatcaattgc cttgcacaag tacacgtagg aaaacataaa
     2941 acattgcaat tttgaatatt gagccttttg tcgtaacatt ggttgatagg attactcacc
     3001 gaatggtttt gaaaccactg ccgacagatc aatcaatcaa tcaaaaaacg tgaactttga
     3061 aaaaggggaa gaacagatac attgaagtta gccatttcca ctgatcgtca caacatatct
     3121 gataaattac tttcaaaatt ataagctgat gtgtgtgtat tattaatgtg acagtaacat
     3181 cccaaacgag aaatattatg tcgacaacaa aaaagtttga tctgaattga aaatgaagtt
     3241 ttcccaccct acccatttgt catattgaaa ccaatcaact gattaatcaa tcaattagaa
     3301 ttgaagctaa actaaaacat accaccgtcc attttgaatg attatatttt tttaatatta
     3361 atatcgagat aatgtttcta agaaagaaag aaaaccagga gtgaaaatta gaaaaggaaa
     3421 ggaaaggaaa aaaagaaaaa tctgaaaata tataaaaaaa aattgtttcg ttggcaataa
     3481 atcttggtga gaacagcgac cgaaagcaaa taagaacaaa atatgagtgt attacgttga
     3541 acaactaatt aacgtgtgtg tatggatctc tttttctttt ttctctttaa ccgactataa
     3601 acaacaaaca tttttgggca gtgcacacac tacttaatat acacagcata aattacacga
     3661 ttagaaacaa attagcttat taaaataacc taatcaaacc gaatatttta tggtattatg
     3721 agtaaactat ataatataaa tagcacacac ccacaacaac aacaaaggaa aactaaaagg
     3781 ttttttcttt ttgaaaagat cgttttcttt attattctct agttttgacg cggccgccac
     3841 cgcggtggag ctccaattcg ccctatagtg agtcgtatta caattcactg gccgtcgttt
     3901 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     3961 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     4021 tgcgcagcct gaatggcgaa tggaaattgt aagcgttaat attttgttaa aattcgcgtt
     4081 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     4141 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     4201 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     4261 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     4321 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     4381 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     4441 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     4501 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     4561 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     4621 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     4681 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     4741 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     4801 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     4861 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     4921 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     4981 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     5041 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     5101 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     5161 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     5221 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     5281 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     5341 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     5401 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     5461 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     5521 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     5581 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     5641 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     5701 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     5761 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     5821 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     5881 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     5941 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     6001 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     6061 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     6121 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     6181 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     6241 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     6301 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     6361 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     6421 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     6481 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     6541 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     6601 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     6661 cgccaagctc gaaattaacc ctcactaaag ggaacaaaag ctggtaccgg gccccccctc
     6721 gagctattaa gatcaccagc ctcgtttata ataaacttag tctaatattt cttttggacc
     6781 aaaatagaaa ctgttctcag gtggcaaaaa acagcttaaa gtctgtgtta aaaaaagatc
     6841 tcggctatgc caatcaaaaa ggagtgacat tattatgtaa aagaagaaat gttgtaattc
     6901 cgcgaaacaa caattttaat gcttactgtg ccgccaccaa tatcctatgg ccaaggggcc
     6961 tttaaacatg ccacggaaat atacagtcaa agtaaaccta ctatgtcttt agagccttca
     7021 ggatcaattg tttttaaatg aatgactgca aaactagaaa agtagcctct tacataagca
     7081 agtcatttgt acaccgagag tttagtaacc agtgaagcaa gtatatctgg caaacctgaa
     7141 acttatctgt gaattataaa gctcataagt aaaaagtgta gaaaagaaga taaggcagac
     7201 caaatacaaa tttgcaacag tacaacgaat acttcagcgt caaaactaga gaataataaa
     7261 gaaaacgatc ttttcaaaaa gaaaaaacct tttagttttc ctttgttgtt gttgtg
//
