LOCUS       sequence-348606-        3986 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid with p15A origin for constitutive mCherry expression in E. 
            coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3986)
  TITLE     Plasmids with p15A origin for constitutive fluorescent protein 
            expression in E. coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pmr101A-cPr-mCherry (ErmR)
FEATURES             Location/Qualifiers
     source          1..3986
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(480..527)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     523..541
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(609..1319)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(887..906)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1156..1174
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(1441..1460)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     rep_origin      1636..2091
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1723..1742)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1933..1954
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      2670..3215
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     3116..3135
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3332..3349
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gctcttgcac actcaagtct cgattcagca attgcttaag ctgccagcgg aatgctttca
       61 tcctaaacca aaagtaaaca gtgtcttaat aaaacttacc cgccatacca cagatgttcc
      121 agataaatat tggaagctat atacgtactt tgtttcaaaa tgggtcaatc gagaatatcg
      181 tcaactgttt actaaaaatc agtttcatca agcaatgaaa cacgccaaag taaacaattt
      241 aagtaccatt acttatgagc aagtattgtc tatttttaat agttatctat tatttaacgg
      301 gaggaaataa ttctatgagt cgctttttta aatttggaaa gttacacgtt actaaaggga
      361 atggagataa attattagat atactactga cagcttccaa gaagctaaag aggtccctag
      421 cgcctacggg gaatttgtat cgatgatatc ccatatccgg atatagttcc tcctttcagc
      481 aaaaaacccc tcaagacccg tttagaggcc ccaaggggtt atgctagtta ttgctcagcg
      541 gtggcagcag ccaactcagc ttcctttcgg gctttgttag cagccggatc caagcttgag
      601 ctcgattact acttgtacag ctcgtccatg ccgccggtgg agtggcggcc ctcggcgcgt
      661 tcgtactgtt ccacgatggt gtagtcctcg ttgtgggagg tgatgtccaa cttgatgttg
      721 acgttgtagg cgccgggcag ctgcacgggc ttcttggcct tgtaggtggt cttgacctca
      781 gcgtcgtagt ggccgccgtc cttcagcttc agcctctgct tgatctcgcc cttcagggcg
      841 ccgtcctcgg ggtacatccg ctcggaggag gcctcccagc ccatggtctt cttctgcatt
      901 acggggccgt cggaggggaa gttggtgccg cgcagcttca ccttgtagat gaactcgccg
      961 tcctgcaggg aggagtcctg ggtcacggtc accacgccgc cgtcctcgaa gttcatcacg
     1021 cgctcccact tgaagccctc ggggaaggac agcttcaagt agtcggggat gtcggcgggg
     1081 tgcttcacgt aggccttgga gccgtacatg aactgagggg acaggatgtc ccaggcgaag
     1141 ggcagggggc cacccttggt caccttcagc ttggcggtct gggtgccctc gtaggggcgg
     1201 ccctcgccct cgccctcgat ctcgaactcg tggccgttca cggagccctc catgtgcacc
     1261 ttgaagcgca tgaactcctt gatgatggcc atgttatcct cctcgccctt gctcaccatc
     1321 tcgagctcct tcgcatatta tatgtgaatc acagtgatat gtcaagtatt agatcttcga
     1381 tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg
     1441 cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg
     1501 cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct
     1561 ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct
     1621 gcttcctaat tgaggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     1681 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     1741 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     1801 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg
     1861 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     1921 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcgggct
     1981 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     2041 tttaacaaaa atttaacgcg aattttaaca aaatattgac gtttacaatt taaatatttg
     2101 cttatacaat cttcctgttt ttggggcttt tcgcaggagt cgcataaggg agagcgtcga
     2161 ccgatgccct tgagagcctt caaccctgca gcaatggcaa caacgttgcg caaactatta
     2221 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     2281 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     2341 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     2401 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     2461 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     2521 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     2581 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     2641 gcgtcagacc ccttaataag atgatcttct tgagatcgtt ttggtctgcg cgtaatctct
     2701 tgctctgaaa acgaaaaaac cgccttgcag ggcggttttt cgaaggttct ctgagctacc
     2761 aactctttga accgaggtaa ctggcttgga ggagcgcagt caccaaaact tgtcctttca
     2821 gtttagcctt aaccggcgca tgacttcaag actaactcct ctaaatcaat taccagtggc
     2881 tgctgccagt ggtgcttttg catgtctttc cgggttggac tcaagacgat agttaccgga
     2941 taaggcgcag cggtcggact gaacgggggg ttcgtgcata cagtccagct tggagcgaac
     3001 tgcctacccg gaactgagtg tcaggcgtgg aatgagacaa acgcggccat aacagcggaa
     3061 tgacaccggt aaaccgaaag gcaggaacag gagagcgcac gagggagccg ccagggggaa
     3121 acgcctggta tctttatagt cctgtcgggt ttcgccacca ctgatttgag cgtcagattt
     3181 cgtgatgctt gtcagggggg cggagcctat ggaaaaacgg ctttgccgcg gccctctcac
     3241 ttccctgtta agtatcttcc tggcatcttc caggaaatct ccgccccgtt cgtaagccat
     3301 ttccgctcgc cgcagtcgaa cgaccgagcg tagcgagtca gtgagcgagg aagcggaata
     3361 tatcctgtat cacatattct gctgacgcac cggtgcagcc ttttttctcc tgccacatga
     3421 agcacttcac tgacaccctc atcagtgcca acatagtaag ccagtataca ctccgctagc
     3481 cttagaagca aacttaagag tgtgttgata gtgcagtatc ttaaaatttt gtgtataata
     3541 ggaattgaag ttaaattaga tgctaaaaat ttgtaattaa gaaggaggga ttcgtcatgt
     3601 tggtattcca aatgcgtaat gtagataaaa catctactgt tttgaaacag actaaaaaca
     3661 gtgattacgc agataaataa atacgttaga ttaattccta ccagtgacta atcttatgac
     3721 tttttaaaca gataactaaa attacaaaca aatcgtttaa cttctgtatt tatttacaga
     3781 tgtaatcact tcaggagtaa ttacatgaac aaaaatataa aatattctca aaacttttta
     3841 acgagtgaaa aagtactcaa ccaaataata aaacaattga atttaaaaga aaccgatacc
     3901 gtttacgaaa ttggaacagg taaagggcat ttaacgacga aactggctaa aataagtaaa
     3961 caggtaacgt ctattgaatt agacag
//
