LOCUS       sequence-348614-        7392 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7392)
  AUTHORS   Chih B, Gollan L, Scheiffele P
  TITLE     Alternative splicing controls selective trans-synaptic interactions 
            of the neuroligin-neurexin complex.
  JOURNAL   Neuron. 2006 Jul 20. 51(2):171-8.
  PUBMED    16846852
REFERENCE   2  (bases 1 to 7392)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2006 Jul 20. 51(2):171-8."
COMMENT     Sequence Label: pCAG-NL1(-)
FEATURES             Location/Qualifiers
     source          1..7392
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          646..1662
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1586..1608
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1670..1689
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1907..1933
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      2329..2338
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      4272..4281
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4396..4415)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4461..4516
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4515..4534)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4877..4893)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4877..4893)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4890..4912)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4901..4917
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4925..4931,4932..4949,4950..4955))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4970..4991
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5050..5245
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5096..5231
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5251..5385
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5288..5307)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5342..5361
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5453..5470)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5624..6212)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5704..5723)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6383..7174,7175..7243))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7006..7025
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7244..7348)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        join(7379..7392,1..366)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
       61 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
      121 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      181 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      241 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      301 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      361 accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca
      421 cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg
      481 gggggggggc gcgcgccrgg gggggggggg sggggggggg ggsggggsgg ggsgrggsgg
      541 rgrggkgsgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg
      601 cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcgggagt cgctgcgcgc
      661 tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg
      721 accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag
      781 cgcttggttt aatgacggct tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc
      841 cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg
      901 gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg
      961 gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg
     1021 tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga
     1081 gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc tgcacccccc tccccgagtt
     1141 gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc
     1201 gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc
     1261 ggggrgggct cgggggaggg gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg
     1321 gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg
     1381 tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc
     1441 ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg
     1501 ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg ggacggctgc
     1561 cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggctctaga
     1621 gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg
     1681 gttattgtgc tgtctcatca ttttggcaaa gaattcgcgg ccgctagcgc taccggactc
     1741 agatctcgag ctcaagcttg ggaccatggc acttcccaga tgcatgtggc caaattatgt
     1801 ttggagagct atgatggcat gtgtggtcca caggggatcc ggtgccccat tgactctctg
     1861 cttgttggga tgtttgctac agacttttca cgtactctct caaaagtatc catacgacgt
     1921 tccggactac gcattggatg atgtagaccc attggttact actaactttg gcaagattag
     1981 gggaattaag aaagaactca ataatgaaat tttgggtcct gtcattcagt ttcttggggt
     2041 tccatatgcc gctccaccaa caggagaaca tcgtttccag cctccagaac caccatctcc
     2101 ctggtctgac atccggaacg ccactcagtt tgctcctgta tgtccccaga atatcattga
     2161 tggcagattg cctgaagtta tgcttcctgt gtggttcact aataacttgg atgtggtttc
     2221 atcatacgtc caagaccaga gtgaagactg tctatactta aacatctatg tcccaactga
     2281 agatgatatt cgggacagtg ggggtcccaa accagtgatg gtgtacatcc atggcggctc
     2341 ttacatggaa ggtactggaa atctgtatga tgggagtgtc ttggcaagct atggcaatgt
     2401 gatcgtcatc acagtcaact atcggcttgg ggtacttggc ttcttgagca caggggatca
     2461 ggctgccaaa ggaaactacg ggctccttga cctcatccag gccctaagat ggaccagcga
     2521 gaacattggg ttctttggtg gtgacccctt gcgaatcact gtgtttggat caggcgctgg
     2581 gggttcatgt gtcaacctgc tgactttatc ccattattct gaaggacttt ttcaacgagc
     2641 aatagctcag agtggaacag ccctttccag ctgggctgtt agtttccagc ctgcaaaata
     2701 cgctagaatt ctggccacaa aagttggctg caatgtttca gatacagtag agttagtaga
     2761 atgcctgcag aagaagcctt acaaagaact tgttgatcaa gatgttcaac cagcccgata
     2821 ccacatagcc tttggacctg tgatcgatgg tgatgtaata ccagatgacc ctcagatact
     2881 gatggaacaa ggagagttcc tcaactatga tataatgttg ggagttaacc aaggggaagg
     2941 gttgaagttt gtcgaaaata tagtagatag tgatgatggt gtatcagcca gtgatttcga
     3001 ctttgctgtt tctaattttg ttgataattt atatggatat cctgaaggca aagatgtttt
     3061 gagagaaacc attaaattca tgtatactga ctgggctgat cgccataatc ctgaaactag
     3121 aaggaagaca ttgttggctt tgtttacgga ccatcaatgg gtagcacctg ctgtggccac
     3181 agcagacctt cactcgaact ttggctcacc tacatacttc tatgcctttt atcatcattg
     3241 ccaaacagat caagttccag cttgggctga tgcagctcat ggggatgagg ttccctatgt
     3301 gttgggaatc cccatgattg gccctacaga gttatttcct tgcaatttct ccaagaatga
     3361 tgtgatgttg agtgcagtag taatgacata ctggacgaat tttgctaaaa ctggtgaccc
     3421 aaatcaacca gttcctcaag acacaaaatt catccatacc aaacccaacc gctttgaaga
     3481 agtagcatgg accagatatt cccagaaaga ccagctttat ctccatattg gattaaaacc
     3541 gagagttaaa gagcattaca gagccaataa ggtaaatctc tggctggagc tggtacctca
     3601 tctgcataat ctcaatgaca tttctcagta tacctcgaca acaactaaag tgccatccac
     3661 ggacatcact ctcagaccta caaggaagaa ttccacacca gtcacatcag cctttcccac
     3721 tgccaaacag gatgatccca agcaacaacc aagccccttc tcggtggatc agagggacta
     3781 ctccacagag ctaagtgtca ctatcgcagt gggggcctct ctgctgtttc tcaacatctt
     3841 ggcttttgca gccctgtact acaagaagga taagaggaga catgatgtcc accggaggtg
     3901 cagccctcag cgcacgacca ccaacgacct aacccatgct ccagaagagg aaattatgtc
     3961 tctccaaatg aagcacactg acttggatca cgagtgtgag tccatccatc cacatgaggt
     4021 ggttcttcgg accgcctgtc ccccagatta tactctagct atgaggaggt cacctgatga
     4081 tattccacta atgacaccta acaccatcac aatgattccc aacactatac cagggattca
     4141 gcccttacat acattcaaca catttactgg aggacagaat aatacactgc cccatcccca
     4201 cccacacccc cattcacatt caacaaccag ggtatagcca gatgagagag acaaactgat
     4261 taatcccgcg gccatggcgg ccgggagcat gcgacgtcgg gcccggggag ctcaattcac
     4321 tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca
     4381 caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct
     4441 tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
     4501 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa
     4561 tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg
     4621 ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca
     4681 tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc
     4741 tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc tcctctcctg
     4801 actactccca gtcatagctg tccctcttct cttatggaga tccctcgacc tgcagcccaa
     4861 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     4921 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     4981 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     5041 agcggatccg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     5101 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     5161 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     5221 ttttggaggc ctaggctttt gcaaaaagct aacttgttta ttgcagctta taatggttac
     5281 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     5341 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgctg cattaatgaa
     5401 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     5461 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     5521 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     5581 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     5641 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     5701 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     5761 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     5821 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     5881 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     5941 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     6001 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     6061 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     6121 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     6181 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     6241 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     6301 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     6361 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     6421 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     6481 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     6541 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     6601 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     6661 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     6721 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     6781 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     6841 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     6901 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     6961 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     7021 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     7081 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     7141 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     7201 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     7261 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     7321 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcga
     7381 cattgattat tg
//
