LOCUS       sequence-348623-       11011 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11011)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3172
FEATURES             Location/Qualifiers
     source          1..11011
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3765..3789)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3797..3838
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3949..4537
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4002..4022)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4420..4431)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4609..4842
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4920..5041
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4957..4976)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5011..5030
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5081..5216
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5236..5255)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5237..5255)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5265..5282)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5265..5281)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5274..5296)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5423..5878
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5510..5529)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5720..5741
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5904..6008
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6009..6077,6078..6869)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6227..6246)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7040..7628
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7529..7548
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7782..7799
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7916..7937
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7952..7957,7958..7975,7976..7982)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7990..8006
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7995..8017
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8014..8030
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8014..8030
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8049..8069
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8051..8069
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8097..8323
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8324..8504
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8551..8676
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9169..9402
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9587..9631
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9862..10072
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9884..10080
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9931..10066
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10112..10714
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(10112..10131)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10608..10628
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10757..10874
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcatgtctct gtggggtctg gtctccaaga tgcccccaga
     1261 aaaagtgcag cggctctatg tcgactttcc ccaacacctg cggcatcttc tgggtgactg
     1321 gctggagagc cagccctggg agttcctggt cggctccgac gccttctgct gcaacttggc
     1381 tagtgcccta ctttcagaca ctgtccagca ccttcaggcc tcggtgggag agcaggggga
     1441 ggggagcacc atcttgcaac acatcagcac ccttgagagc atatatcaga gggaccccct
     1501 gaagctggtg gccactttca gacaaatact tcaaggagag aaaaaagctg ttatggaaca
     1561 gttccgccac ttgccaatgc ctttccactg gaagcaggaa gaactcaagt ttaagacagg
     1621 cttgcggagg ctgcagcacc gagtagggga gatccacctt ctccgagaag ccctgcagaa
     1681 gggggctgag gctggccaag tgtctctgca cagcttgata gaaactcctg ctaatgggac
     1741 tgggccaagt gaggccctgg ccatgctact gcaggagacc actggagagc tagaggcagc
     1801 caaagcccta gtgctgaaga ggatccagat ttggaaacgg cagcagcagc tggcagggaa
     1861 tggcgcaccg tttgaggaga gcctggcccc actccaggag aggtgtgaaa gcctggtgga
     1921 catttattcc cagctacagc aggaggtagg ggcggctggt ggggagcttg agcccaagac
     1981 ccgggcatcg ctgactggcc ggctggatga agtcctgaga accctcgtca ccagttgctt
     2041 cctggtggag aagcagcccc cccaggtact gaagactcag accaagttcc aggctggagt
     2101 tcgattcctg ttgggcttga ggttcctggg ggccccagcc aagcctccgc tggtcagggc
     2161 cgacatggtg acagagaagc aggcgcggga gctgagtgtg cctcagggtc ctggggctgg
     2221 agcagaaagc actggagaaa tcatcaacaa cactgtgccc ttggagaaca gcattcctgg
     2281 gaactgctgc tctgccctgt tcaagaacct gcttctcaag aagatcaagc ggtgtgagcg
     2341 gaagggcact gagtctgtca cagaggagaa gtgcgctgtg ctcttctctg ccagcttcac
     2401 acttggcccc ggcaaactcc ccatccagct ccaggccctg tctctgcccc tggtggtcat
     2461 cgtccatggc aaccaagaca acaatgccaa agccactatc ctgtgggaca atgccttctc
     2521 tgagatggac cgcgtgccct ttgtggtggc tgagcgggtg ccctgggaga agatgtgtga
     2581 aactctgaac ctgaagttca tggctgaggt ggggaccaac cgggggctgc tcccagagca
     2641 cttcctcttc ctggcccaga agatcttcaa tgacaacagc ctcagtatgg aggccttcca
     2701 gcaccgttct gtgtcctggt cgcagttcaa caaggagatc ctgctgggcc gtggcttcac
     2761 cttttggcag tggtttgatg gtgtcctgga cctcaccaaa cgctgtctcc ggagctactg
     2821 gtctgaccgg ctgatcattg gcttcatcag caaacagtac gttactagcc ttcttctcaa
     2881 tgagcccgac ggaacctttc tcctccgctt cagcgactca gagattgggg gcatcaccat
     2941 tgcccatgtc atccggggcc aggatggctc tccacagata gagaacatcc agccattctc
     3001 tgccaaagac ctgtccattc gctcactggg ggaccgaatc cgggatcttg ctcagctcaa
     3061 aaatctctat cccaagaagc ccaaggatga ggctttccgg agccactaca agcctgaaca
     3121 gatgggtaag gatggcaggg gttatgtccc agctaccatc aagatgaccg tggaaaggga
     3181 ccaaccactt cctaccccag agctccagat gcctaccatg gtgccttctt atgaccttgg
     3241 aatggcccct gattcctcca tgagcatgca gcttggccca gatatggtgc cccaggtgta
     3301 cccaccacac tctcactcca tccccccgta tcaaggcctc tccccagaag aatcagtcaa
     3361 cgtgttgtca gccttccagg agcctcacct gcagatgccc cccagcctgg gccagatgag
     3421 cctgcccttt gaccagcctc acccccaggg cctgctgccg tgccagcctc aggagcatgc
     3481 tgtgtccagc cctgaccccc tgctctgctc agatgtgacc atggtggaag acagctgcct
     3541 gagccagcca gtgacagcgt ttcctcaggg cacttggatt ggtgaagaca tattccctcc
     3601 tctgctgcct cccactgaac aggacctcac taagcttctc ctggaggggc aaggggagtc
     3661 ggggggaggg tccttggggg cacagcccct cctgcagccc tcccactatg ggcaatctgg
     3721 gatctcaatg tcccacatgg acctaagggc caaccccagt tggtgcccaa ctttcttgta
     3781 caaagtggtt gatatcggta agcctatccc taaccctctc ctcggtctcg attctacgta
     3841 gtaatgaact agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
     3901 aaaggacgat cttaatttag gttactgtgt taaacgcgtt aagtcgacaa tcaacctctg
     3961 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     4021 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     4081 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     4141 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     4201 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     4261 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     4321 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     4381 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     4441 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     4501 cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt
     4561 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     4621 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     4681 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     4741 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     4801 gatccctcag acccttttag tcagtgtgga aaatctctag cagtacgtat agtagttcat
     4861 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
     4921 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     4981 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     5041 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
     5101 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     5161 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     5221 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     5281 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     5341 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     5401 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     5461 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     5521 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     5581 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     5641 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     5701 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     5761 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     5821 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
     5881 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     5941 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     6001 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     6061 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     6121 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     6181 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     6241 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     6301 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     6361 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     6421 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     6481 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     6541 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     6601 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     6661 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     6721 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     6781 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     6841 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     6901 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     6961 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     7021 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     7081 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     7141 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     7201 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     7261 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     7321 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     7381 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     7441 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     7501 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     7561 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     7621 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     7681 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     7741 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     7801 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     7861 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     7921 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     7981 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     8041 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta
     8101 gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt
     8161 acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc
     8221 cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca
     8281 ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga
     8341 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     8401 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     8461 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     8521 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     8581 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     8641 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     8701 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     8761 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     8821 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     8881 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     8941 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     9001 accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     9061 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     9121 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     9181 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     9241 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     9301 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     9361 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     9421 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     9481 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     9541 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     9601 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     9661 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     9721 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     9781 ccacctccca accccgaggg gacccatgca ttgcatctca attagtcagc aaccaggtgt
     9841 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcgtct caattagtca
     9901 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     9961 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
    10021 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
    10081 aagctttcta gaggtaccac cggagcttac catgaccgag tacaagccca cggtgcgcct
    10141 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
    10201 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
    10261 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
    10321 cgccgccgtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
    10381 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
    10441 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
    10501 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
    10561 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
    10621 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
    10681 ctggtgcatg acccgcaagc ccggtgccag ttaacgaatt ctagatcttg agacaaatgg
    10741 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
    10801 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
    10861 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg cgccggctcg
    10921 aggcctgcag gtgcaaagat ggataaagtt ttaaacagag aggaatcttt gcagctaatg
    10981 gaccttctag gtcttgaaag gagtgggaat t
//
