LOCUS       sequence-348625-       11017 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11017)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3173
FEATURES             Location/Qualifiers
     source          1..11017
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3771..3795)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3803..3844
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3955..4543
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4008..4028)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4426..4437)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4615..4848
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4926..5047
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4963..4982)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5017..5036
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5087..5222
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5242..5261)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5243..5261)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5271..5288)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5271..5287)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5280..5302)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5429..5884
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5516..5535)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5726..5747
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5910..6014
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6015..6083,6084..6875)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6233..6252)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7046..7634
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7535..7554
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7788..7805
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7922..7943
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7958..7963,7964..7981,7982..7988)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7996..8012
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8001..8023
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8020..8036
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8020..8036
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8055..8075
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8057..8075
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8103..8329
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8330..8510
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8557..8682
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9175..9408
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9593..9637
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9868..10078
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9890..10086
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9937..10072
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10118..10720
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(10118..10137)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10614..10634
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10763..10880
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagcag gcaccatgtc tctgtggggt ctggtctcca agatgccccc
     1261 agaaaaagtg cagcggctct atgtcgactt tccccaacac ctgcggcatc ttctgggtga
     1321 ctggctggag agccagccct gggagttcct ggtcggctcc gacgccttct gctgcaactt
     1381 ggctagtgcc ctactttcag acactgtcca gcaccttcag gcctcggtgg gagagcaggg
     1441 ggaggggagc accatcttgc aacacatcag cacccttgag agcatatatc agagggaccc
     1501 cctgaagctg gtggccactt tcagacaaat acttcaagga gagaaaaaag ctgttatgga
     1561 acagttccgc cacttgccaa tgcctttcca ctggaagcag gaagaactca agtttaagac
     1621 aggcttgcgg aggctgcagc accgagtagg ggagatccac cttctccgag aagccctgca
     1681 gaagggggct gaggctggcc aagtgtctct gcacagcttg atagaaactc ctgctaatgg
     1741 gactgggcca agtgaggccc tggccatgct actgcaggag accactggag agctagaggc
     1801 agccaaagcc ctagtgctga agaggatcca gatttggaaa cggcagcagc agctggcagg
     1861 gaatggcgca ccgtttgagg agagcctggc cccactccag gagaggtgtg aaagcctggt
     1921 ggacatttat tcccagctac agcaggaggt aggggcggct ggtggggagc ttgagcccaa
     1981 gacccgggca tcgctgactg gccggctgga tgaagtcctg agaaccctcg tcaccagttg
     2041 cttcctggtg gagaagcagc ccccccaggt actgaagact cagaccaagt tccaggctgg
     2101 agttcgattc ctgttgggct tgaggttcct gggggcccca gccaagcctc cgctggtcag
     2161 ggccgacatg gtgacagaga agcaggcgcg ggagctgagt gtgcctcagg gtcctggggc
     2221 tggagcagaa agcactggag aaatcatcaa caacactgtg cccttggaga acagcattcc
     2281 tgggaactgc tgctctgccc tgttcaagaa cctgcttctc aagaagatca agcggtgtga
     2341 gcggaagggc actgagtctg tcacagagga gaagtgcgct gtgctcttct ctgccagctt
     2401 cacacttggc cccggcaaac tccccatcca gctccaggcc ctgtctctgc ccctggtggt
     2461 catcgtccat ggcaaccaag acaacaatgc caaagccact atcctgtggg acaatgcctt
     2521 ctctgagatg gaccgcgtgc cctttgtggt ggctgagcgg gtgccctggg agaagatgtg
     2581 tgaaactctg aacctgaagt tcatggctga ggtggggacc aaccgggggc tgctcccaga
     2641 gcacttcctc ttcctggccc agaagatctt caatgacaac agcctcagta tggaggcctt
     2701 ccagcaccgt tctgtgtcct ggtcgcagtt caacaaggag atcctgctgg gccgtggctt
     2761 caccttttgg cagtggtttg atggtgtcct ggacctcacc aaacgctgtc tccggagcta
     2821 ctggtctgac cggctgatca ttggcttcat cagcaaacag tacgttacta gccttcttct
     2881 caatgagccc gacggaacct ttctcctccg cttcagcgac tcagagattg ggggcatcac
     2941 cattgcccat gtcatccggg gccaggatgg ctctccacag atagagaaca tccagccatt
     3001 ctctgccaaa gacctgtcca ttcgctcact gggggaccga atccgggatc ttgctcagct
     3061 caaaaatctc tatcccaaga agcccaagga tgaggctttc cggagccact acaagcctga
     3121 acagatgggt aaggatggca ggggttatgt cccagctacc atcaagatga ccgtggaaag
     3181 ggaccaacca cttcctaccc cagagctcca gatgcctacc atggtgcctt cttatgacct
     3241 tggaatggcc cctgattcct ccatgagcat gcagcttggc ccagatatgg tgccccaggt
     3301 gtacccacca cactctcact ccatcccccc gtatcaaggc ctctccccag aagaatcagt
     3361 caacgtgttg tcagccttcc aggagcctca cctgcagatg ccccccagcc tgggccagat
     3421 gagcctgccc tttgaccagc ctcaccccca gggcctgctg ccgtgccagc ctcaggagca
     3481 tgctgtgtcc agccctgacc ccctgctctg ctcagatgtg accatggtgg aagacagctg
     3541 cctgagccag ccagtgacag cgtttcctca gggcacttgg attggtgaag acatattccc
     3601 tcctctgctg cctcccactg aacaggacct cactaagctt ctcctggagg ggcaagggga
     3661 gtcgggggga gggtccttgg gggcacagcc cctcctgcag ccctcccact atgggcaatc
     3721 tgggatctca atgtcccaca tggacctaag ggccaacccc agttggtaga acccagcttt
     3781 cttgtacaaa gtggttgata tcggtaagcc tatccctaac cctctcctcg gtctcgattc
     3841 tacgtagtaa tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc
     3901 ttgtggaaag gacgatagtg acaggccaca ttactatgta cgcgttaagt cgacaatcaa
     3961 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     4021 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     4081 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     4141 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     4201 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     4261 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     4321 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     4381 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     4441 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     4501 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa
     4561 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     4621 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     4681 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     4741 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     4801 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta
     4861 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     4921 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     4981 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     5041 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     5101 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     5161 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     5221 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     5281 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     5341 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     5401 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     5461 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     5521 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     5581 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     5641 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     5701 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     5761 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     5821 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     5881 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     5941 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     6001 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     6061 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     6121 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     6181 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     6241 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     6301 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     6361 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     6421 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     6481 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     6541 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     6601 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     6661 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     6721 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     6781 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     6841 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     6901 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     6961 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     7021 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     7081 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     7141 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     7201 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     7261 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     7321 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     7381 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     7441 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     7501 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     7561 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     7621 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     7681 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     7741 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     7801 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     7861 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     7921 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     7981 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     8041 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
     8101 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
     8161 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
     8221 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
     8281 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
     8341 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     8401 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     8461 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     8521 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     8581 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     8641 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     8701 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     8761 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     8821 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     8881 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     8941 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     9001 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
     9061 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     9121 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     9181 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     9241 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     9301 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     9361 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     9421 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     9481 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     9541 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     9601 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     9661 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     9721 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     9781 tcagacccac ctcccaaccc cgaggggacc catgcattgc atctcaatta gtcagcaacc
     9841 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcgtctcaat
     9901 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     9961 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
    10021 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
    10081 tgcaaaaagc tttctagagg taccaccgga gcttaccatg accgagtaca agcccacggt
    10141 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
    10201 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
    10261 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
    10321 cgacggcgcc gccgtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
    10381 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
    10441 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
    10501 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
    10561 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
    10621 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
    10681 gcgcacctgg tgcatgaccc gcaagcccgg tgccagttaa cgaattctag atcttgagac
    10741 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
    10801 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
    10861 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc
    10921 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
    10981 ctaatggacc ttctaggtct tgaaaggagt gggaatt
//
