LOCUS       sequence-348629-       12084 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12084)
  AUTHORS   Guo W, Keckesova Z, Donaher JL, Shibue T, Tischler V, Reinhardt F, 
            Itzkovitz S, Noske A, Zurrer-Hardi U, Bell G, Tam WL, Mani SA, van 
            Oudenaarden A, Weinberg RA
  TITLE     Slug and Sox9 cooperatively determine the mammary stem cell state.
  JOURNAL   Cell. 2012 Mar 2;148(5):1015-28.
  PUBMED    22385965
REFERENCE   2  (bases 1 to 12084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2012-03-2"; volume: "148(5)"; pages: "1015-28"
COMMENT     Sequence Label: pWPXL-Sox4
FEATURES             Location/Qualifiers
     source          1..12084
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2145..3323
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2376..3314
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3271..3291
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3371..3488
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    3578..3602
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     3603..3620
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3604..3620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5830..5846)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5830..5846)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(5847..5871)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5915..6503
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5968..5988)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6386..6397)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(6647..6680)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             6700..6880
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6905..6923)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6906..6923)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7213..7232)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7446..7465)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7565..7587
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7625..7643)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7711..7815
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7816..7884,7885..8676)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8034..8053)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8847..9435
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9336..9355
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9589..9606
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9676..9696)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9681..10010
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9861..9996
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11144..11209
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11339..11359
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11784..11918
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11821..11840)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11875..11894
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg
     2041 accatcgatg tcgacgataa gctttgcaaa gatggataaa gttttaaaca gagaggaatc
     2101 tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc
     2161 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt
     2221 gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc
     2281 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg
     2341 ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg
     2401 ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc
     2461 agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt
     2521 gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc
     2581 gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag
     2641 ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa
     2701 atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg
     2761 cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa
     2821 tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt
     2881 gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa
     2941 gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag
     3001 agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt
     3061 catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt
     3121 ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg
     3181 agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg
     3241 ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt
     3301 ttcttccatt tcaggtgtcg tgaggaattt cgacatttaa atttaattaa tctcgacggt
     3361 atcggttaac ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     3421 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     3481 aaaattttat cgatcacgag actagcctcg aggtttaaac tacgggatcc aggcctaagc
     3541 ttacgcgtcc cagtgtggtg gaattctgca gatatcaaca agtttgtaca aaaaagcagg
     3601 cttgtaaaac gacggccagt aactataacg gtcctaaggt agcgaggcct gggtggcgaa
     3661 tcggcacgag gctgcactgg aggaactcct gccattacca gctcccttct tgcagaaggg
     3721 agggggaaac atacatttat tcatgccagt ctgttgcatg caggcttttt ggcttcctac
     3781 cttgcaacaa aataattgca ccaactcctt agtgccgatt ccgcccacag agagtcctgg
     3841 agccacagtc ttttttgctt tgcattgtag gagagggact aagtgctaga gactatgtcg
     3901 ctttcctgag ctaccgagag cgctcgtgaa ctggaatcaa ctgcttcagg gaaaaagaaa
     3961 aaaaaaaaaa aaagacttgc ctgggaggcc gcgagaaact tgcattggaa gcttcagcaa
     4021 ccagcattcg agaaactcct ctctacttta gcacggtctc cagactcagc cgagagacag
     4081 caaactgcag cgcggtgaga gagcgagaga gagggagaga gagactctcc agcctgggaa
     4141 ctataactcc tctgcgagag gcggagaact ccttccccaa atcttttggg gacttttctc
     4201 tctttaccca cctccgcccc tgcgaggagt tgaggcggcc gccgcgaggg tgtgagcgcg
     4261 cgtgggcgcc cgccgagccg aggccatggt gcagcaaacc aacaatgccg agaacacgga
     4321 agcgctgctg gccggcgaga gctcggactc gggcgccggc ctcgagctgg gaatcgcctc
     4381 ctcccccacg cccggctcca ccgcctccac gggcggcaag gccgacgacc cgagctggtg
     4441 caagaccccg agtgggcaca tcaagcgacc catgaacgcc ttcatggtgt ggtcgcagat
     4501 cgagcggcgc aagatcatgg agcagtcgcc cgacatgcac aacgccgaga tctccaagcg
     4561 gctgggcaaa cgctggaagc tgctcaaaga cagcgacaag atccctttca ttcgagaggc
     4621 ggagcggctg cgcctcaagc acatggctga ctaccccgac tacaagtacc ggcccaggaa
     4681 gaaggtgaag tccggcaacg ccaactccag ctcctcggcc gccgcctcct ccaagccggg
     4741 ggagaaggga gacaaggtcg gtggcagtgg cgggggcggc catgggggcg gcggcggcgg
     4801 cgggagcagc aacgcggggg gaggaggcgg cggtgcgagt ggcggcggcg ccaactccaa
     4861 accggcgcag aaaaagagct gcggctccaa agtggcgggc ggcgcgggcg gtggggttag
     4921 caaaccgcac gccaagctca tcctggcagg cggcggcggc ggcgggaaag cagcggctgc
     4981 cgccgccgcc tccttcgccg ccgaacaggc gggggccgcc gccctgctgc ccctgggcgc
     5041 cgccgccgac caccactcgc tgtacaaggc gcggactccc agcgcctcgg cctccgcctc
     5101 ctcggcagcc tcggcctccg cagcgctcgc ggccccgggc aagcacctgg cggagaagaa
     5161 ggtgaagcgc gtctacctgt tcggcggcct gggcacgtcg tcgtcgcccg tgggcggcgt
     5221 gggcgcggga gccgacccca gcgaccccct gggcctgtac gaggaggagg gcgcgggctg
     5281 ctcgcccgac gcgcccagcc tgagcggccg cagcagcgcc gcctcgtccc ccgccgccgg
     5341 ccgctcgccc gccgaccacc gcggctacgc cagcctgcgc gccgcctcgc ccgccccgtc
     5401 cagcgcgccc tcgcacgcgt cctcctcggc ctcgtcccac tcctcctctt cctcctcctc
     5461 gggctcctcg tcctccgacg acgagttcga agacgacctg ctcgacctga accccagctc
     5521 aaactttgag agcatgtccc tgggcagctt cagttcgtcg tcggcgctcg accgggacct
     5581 ggattttaac ttcgagcccg gctccggctc gcacttcgag ttcccggact actgcacgcc
     5641 cgaggtgagc gagatgatct cgggagactg gctcgagtcc agcatctcca acctggtttt
     5701 cacctactga agggcgcgca ggcagggaga agggccgggg ggggtaggag aggagaaaaa
     5761 aaaaaaaaaa aaaaaaaact cgaggcatct atgtcgggtg cggagaaaga ggtaatgaaa
     5821 tggcacatgg tcatagctgt ttcctgaccc agctttcttg tacaaagtgg ttgatatcca
     5881 gcacagtggt cgacgataaa actagtcata tgataatcaa cctctggatt acaaaatttg
     5941 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     6001 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     6061 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     6121 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     6181 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     6241 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     6301 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
     6361 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     6421 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     6481 ctccctttgg gccgcctccc cgcatcggta cgtatggcca ggtaccttta agaccaatga
     6541 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     6601 taattcactc ccaacgaaga caagatggga tcaattcacc atgggaataa cttcgtatag
     6661 catacattat acgaagttat gctgcttttt gcttgtactg ggtctctctg gttagaccag
     6721 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     6781 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     6841 tccctcagac ccttttagtc agtgtggaaa atctctagca gcatctagaa ttaattccgt
     6901 gtattctata gtgtcaccta aatcgtatgt gtatgataca taaggttatg tattaattgt
     6961 agccgcgttc taacgacaat atgtacaagc ctaattgtgt agcatctggc ttactgaagc
     7021 agaccctatc atctctctcg taaactgccg tcagagtcgg tttggttgga cgaaccttct
     7081 gagtttctgg taacgccgtc ccgcacccgg aaatggtcag cgaaccaatc agcagggtca
     7141 tcgctagcca gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg
     7201 cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg
     7261 ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt
     7321 tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc
     7381 tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga atggtgcact
     7441 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     7501 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     7561 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     7621 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     7681 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     7741 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     7801 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     7861 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     7921 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     7981 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     8041 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     8101 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     8161 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     8221 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     8281 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     8341 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     8401 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     8461 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     8521 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     8581 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     8641 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     8701 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     8761 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     8821 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     8881 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     8941 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     9001 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     9061 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     9121 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     9181 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     9241 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     9301 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     9361 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     9421 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     9481 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     9541 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     9601 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     9661 cattaatgca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     9721 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     9781 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     9841 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     9901 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     9961 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttggac
    10021 acaagacagg cttgcgagat atgtttgaga ataccacttt atcccgcgtc agggagaggc
    10081 agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc agatctctat
    10141 aatctcgcgc aacctatttt cccctcgaac actttttaag ccgtagataa acaggctggg
    10201 acacttcaca tgagcgaaaa atacatcgtc acctgggaca tgttgcagat ccatgcacgt
    10261 aaactcgcaa gccgactgat gccttctgaa caatggaaag gcattattgc cgtaagccgt
    10321 ggcggtctgt accgggtgcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg
    10381 tttgtatttc cagctacgat cacgacaacc agcgcgagct taaagtgctg aaacgcgcag
    10441 aaggcgatgg cgaaggcttc atcgttattg atgacctggt ggataccggt ggtactgcgg
    10501 ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac catcttcgca aaaccggctg
    10561 gtcgtccgct ggttgatgac tatgttgttg atatcccgca agatacctgg attgaacagc
    10621 cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc
    10681 tggcactgcc gggcgttgtt ctttttaact tcaggcgggt tacaatagtt tccagtaagt
    10741 attctggagg ctgcatccat gacacaggca aacctgagcg aaaccctgtt caaaccccgc
    10801 tttaaacatc ctgaaacctc gacgctagtc cgccgcttta atcacggcgc acaaccgcct
    10861 gtgcagtcgg cccttgatgg taaaaccatc cctcactggt atcgcatgat taaccgtctg
    10921 atgtggatct ggcgcggcat tgacccacgc gaaatcctcg acgtccaggc acgtattgtg
    10981 atgagcgatg ccgaacgtac cgacgatgat ttatacgata cggtgattgg ctaccgtggc
    11041 ggcaactgga tttatgagtg ggccccggat ctttgtgaag gaaccttact tctgtggtgt
    11101 gacataattg gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt
    11161 aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta
    11221 tggaactgat gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc
    11281 agaagaaatg ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc
    11341 aaaaaagaag agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt
    11401 gagtcatgct gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga
    11461 aaaagctgca ctgctataca agaaaattat ggaaaaatat tctgtaacct ttataagtag
    11521 gcataacagt tataatcata acatactgtt ttttcttact ccacacaggc atagagtgtc
    11581 tgctattaat aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt
    11641 taataaggaa tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat
    11701 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
    11761 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
    11821 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
    11881 tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc
    11941 aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc
    12001 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt
    12061 aaatatgtac tacaaactta gtag
//
