LOCUS       sequence-348685-       10123 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Kinetochore anchor for TMP-Halo dimerization system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10123)
  AUTHORS   Chen GY, Renda F, Zhang H, Gokden A, Wu DZ, Chenoweth DM, Khodjakov 
            A, Lampson MA
  TITLE     Tension promotes kinetochore-microtubule release by Aurora B kinase.
  JOURNAL   J Cell Biol. 2021 Jun 7;220(6). pii: 212027. doi: 
            10.1083/jcb.202007030.
  PUBMED    33904910
REFERENCE   2  (bases 1 to 10123)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2021-06-7"; volume: "220(6). pii: 212027. doi"; 
            pages: " 10.1083/jcb.202007030"
COMMENT     Sequence Label: CAG.3xHalo-GFP-SPC25
FEATURES             Location/Qualifiers
     source          1..10123
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     421..443
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     505..524
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             573..1463
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             1491..2378
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="AEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
     regulatory      2409..2418
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2415..3305
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     regulatory      3312..3321
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3318..3320,3321..3323,3324..4034)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3318..3320,3321..3323,3324..4034)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3363..3384)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3624..3643)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3971..3992
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4818..4837)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4883..4938
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4937..4956)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     protein_bind    5302..5335
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    5348..5469
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5385..5404)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5439..5458
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5488..5508)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5488..5506)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5527..5543)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5527..5543)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5540..5562)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5551..5567
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5575..5581,5582..5599,5600..5605))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5620..5641
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5758..5775)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5929..6517)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6009..6028)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6688..7479,7480..7548))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7311..7330
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7549..7653)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    7726..7774
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    7986..8019
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             8024..8623
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8024..8043)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8520..8540
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(8759..8776)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8765..8876
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        9021..9324
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9326..9603
                     /label=chicken beta-actin promoter
     intron          join(9604..10123,1..497)
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
ORIGIN
        1 cggggctccg tacggggcgt ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg
       61 tgggggtgcc gggcggggcg gggccgcctc gggccgggga gggctcgggg gaggggcgcg
      121 gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat
      181 ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat
      241 ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca
      301 ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc
      361 tccagcctcg gggctgtccg cgggaggacg gctgccttcg ggggggacgg ggcagggcgg
      421 ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct
      481 tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg
      541 gcaaagaatt gctcgaccac cggcgcgcca caatggcaga gatcggtact ggcttcccat
      601 tcgatcccca ttacgtggaa gtcctgggcg aacgcatgca ctatgtcgat gttggtccgc
      661 gcgacggcac ccctgtgctg ttcctgcatg gtaacccgac ctcctcctat gtgtggcgca
      721 acattatccc gcacgttgca ccgacccatc gctgtattgc tccagatctg atcggtatgg
      781 gcaagtccga caagccagac ctgggttact tctttgacga tcacgtccgc tttatggatg
      841 cctttatcga ggccctgggt ctggaagaag tcgtcctggt cattcatgac tggggctccg
      901 ctctgggttt tcactgggcc aaacgcaatc cagaacgcgt caaaggtatc gcatttatgg
      961 aattcattcg ccctatcccg acctgggacg aatggccaga gtttgcccgc gaaaccttcc
     1021 aggcctttcg caccaccgac gtcggccgca aactgatcat tgatcagaat gtttttattg
     1081 agggtacgct gccgatgggt gtcgtccgcc cgctgactga ggtcgagatg gatcattatc
     1141 gcgagccgtt tctgaatcct gttgatcgcg agccactgtg gcgctttcca aacgaactgc
     1201 caatcgccgg tgaaccagcg aacattgtcg cgctggtcga agagtacatg gattggctgc
     1261 accagtcccc tgtcccgaaa ctgctgttct ggggcacccc aggcgttctg attccaccgg
     1321 ccgaagccgc tcgcctggcc aagagcctgc ctaactgcaa agctgtggac attggcccgg
     1381 gtctgaatct gctgcaagag gacaatccgg acctgattgg cagcgagatt gcgcgctggc
     1441 tgtcgacgct cgagatctcc ggcgcttcga attctgcagt cgacggtacc gcagaaattg
     1501 gtactggctt tccatttgac ccccactatg tggaggtcct gggcgagcgc atgcattacg
     1561 tcgacgttgg tccgcgcgat ggcacccctg tgctgtttct gcacggtaat ccgacctcct
     1621 cctacgtgtg gcgcaatatc attccgcatg ttgcaccgac ccaccgctgc atcgctccag
     1681 acctgattgg tatgggcaaa tccgataaac cagatctggg ttattttttc gatgaccatg
     1741 tccgcttcat ggacgccttc attgaagccc tgggtctgga ggaggtcgtc ctggtcatcc
     1801 acgattgggg ctccgctctg ggtttccatt gggccaagcg caacccagag cgcgtcaagg
     1861 gtattgcatt catggagttt atccgcccta ttccgacctg ggacgagtgg ccagaattcg
     1921 cccgcgagac ctttcaggcc ttccgcacca ccgatgtcgg ccgcaagctg attatcgacc
     1981 agaacgtttt catcgaaggt acgctgccga tgggtgtcgt ccgcccgctg actgaagtcg
     2041 aaatggacca ctaccgcgaa ccgttcctga accctgttga ccgcgaacca ctgtggcgct
     2101 tcccaaatga gctgccaatt gccggtgagc cagcgaatat cgtcgcgctg gtcgaggaat
     2161 atatggactg gctgcatcag tcccctgtcc cgaagctgct gttttggggc accccaggcg
     2221 ttctgatccc accggccgag gccgctcgcc tggccaaaag cctgcctaat tgcaaggctg
     2281 tggatatcgg cccgggtctg aacctgctgc aagaagataa cccggatctg atcggcagcg
     2341 aaatcgcgcg ctggctgtcg acgctcgaaa tttccggcag tgctggtagt gctggtgaat
     2401 tgatgcgcgc caccatggca gaaatcggta ctggctttcc attcgacccc cattatgtgg
     2461 aagtcctggg cgagcgcatg cactacgtcg atgttggtcc gcgcgatggc acccctgtgc
     2521 tgttcctgca cggtaacccg acctcctcct acgtgtggcg caacatcatc ccgcatgttg
     2581 caccgaccca tcgctgcatt gctccagacc tgatcggtat gggcaaatcc gacaaaccag
     2641 acctgggtta tttcttcgac gaccacgtcc gcttcatgga tgccttcatc gaagccctgg
     2701 gtctggaaga ggtcgtcctg gtcattcacg actggggctc cgctctgggt ttccactggg
     2761 ccaagcgcaa tccagagcgc gtcaaaggta ttgcatttat ggagttcatc cgccctatcc
     2821 cgacctggga cgaatggcca gaatttgccc gcgagacctt ccaggccttc cgcaccaccg
     2881 acgtcggccg caagctgatc atcgatcaga acgtttttat cgagggtacg ctgccgatgg
     2941 gtgtcgtccg cccgctgact gaagtcgaga tggaccatta ccgcgagccg ttcctgaatc
     3001 ctgttgaccg cgagccactg tggcgcttcc caaacgagct gccaatcgcc ggtgagccag
     3061 cgaacatcgt cgcgctggtc gaagaataca tggactggct gcaccagtcc cctgtcccga
     3121 agctgctgtt ctggggcacc ccaggcgttc tgatcccacc ggccgaagcc gctcgcctgg
     3181 ccaaaagcct gcctaactgc aaggctgtgg acatcggccc gggtctgaat ctgctgcaag
     3241 aagacaaccc ggacctgatc ggcagcgaga tcgcgcgctg gctgtcgacg ctcgagattt
     3301 ccggttacgt agccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     3361 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     3421 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     3481 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     3541 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     3601 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     3661 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     3721 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     3781 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     3841 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     3901 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     3961 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     4021 acgagctgta caagtccgga ctcagatccc atatggtaga ggacgaactg gcacttttcg
     4081 ataaaagcat aaatgaattt tggaataaat tcaaaagtac ggacacctcc tgtcagatgg
     4141 cgggactaag agatacctac aaggattcca tcaaagcatt tgcagaaaag ctgtctgtga
     4201 aattaaagga agaagaacga atggttgaga tgtttctgga atatcaaaat cagatcagca
     4261 ggcaaaataa gctcattcaa gaaaaaaagg ataacttgtt aaaattgatt gctgaagtaa
     4321 aaggcaaaaa gcaggaattg gaagtactga ctgcaaatat ccaggatctt aaggaagaat
     4381 attctaggaa gaaggaaact atttctactg ctaataaagc gaatgcagag aggttgaaaa
     4441 ggctgcagaa atctgcagac ttgtataaag atcgacttgg actagaaatt cgaaaaattt
     4501 atggtgagaa attgcagttt attttcacta atattgaccc taagaatcct gagagcccat
     4561 ttatgttttc cttacatctc aatgaagcaa gggactatga agtgtcagat agtgcccctc
     4621 atcttgaggg cctagcagaa tttcaagaga atgtaaggaa gaccaacaat ttttcagctt
     4681 ttcttgccaa tgttcggaaa gcttttactg ccacggttta taattaagcg gccgcaattc
     4741 actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa tgccctggct
     4801 cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat catgaagccc
     4861 cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg
     4921 aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag
     4981 aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa
     5041 ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc
     5101 catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt
     5161 tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc
     5221 tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga cctgcaccgt
     5281 cgagctagca tatggatcca tataacttcg tataatgtat gctatacgaa gttattacga
     5341 taccgtcaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     5401 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     5461 tgtatcttag gtacccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta
     5521 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     5581 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     5641 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     5701 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     5761 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     5821 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     5881 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     5941 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     6001 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     6061 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     6121 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     6181 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     6241 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     6301 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     6361 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     6421 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     6481 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     6541 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     6601 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     6661 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     6721 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     6781 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     6841 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     6901 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     6961 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     7021 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     7081 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     7141 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     7201 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     7261 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     7321 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     7381 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     7441 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     7501 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     7561 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     7621 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccaagctt
     7681 aataaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac
     7741 agaataaaac gcacgggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc
     7801 tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc
     7861 ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg
     7921 gcggcaggcc ctgccatagc cactggcccc gtgggttagg gacggggtcc cccattcgag
     7981 aataaataac ttcgtataaa gtatcctata cgaagttatc accatgaccg agtacaagcc
     8041 cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc
     8101 gttcgccgac taccccgcca cgcgccacac cgtcgacccg gaccgccaca tcgagcgggt
     8161 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     8221 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     8281 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     8341 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     8401 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     8461 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     8521 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     8581 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc
     8641 gcagcgcccg accgaaagga gcgcacgacc ccatggctcc gaccgaagcc acccggggcg
     8701 gccccgccga ccccgcaccc gcccccgagg cccaccgact ctagagctcg ctgatcagcc
     8761 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     8821 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     8881 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     8941 gattgggaag acaatagcac tagttattaa tagtaatcaa ttacggggtc attagttcat
     9001 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     9061 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     9121 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     9181 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     9241 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     9301 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
     9361 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
     9421 gcgatggggg cggggggggg gggggggcgc gcgccrggcg gggsggggsg gggcgrgggg
     9481 sggggsgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag
     9541 tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg
     9601 gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc
     9661 ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct
     9721 cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt
     9781 gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg
     9841 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag
     9901 cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc
     9961 gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt
    10021 gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg
    10081 cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtg
//
