LOCUS       sequence-348710-       13430 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Full-length YTHDC1 with mCherry and CRY2 tag for Optodroplet
            Assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13430)
  AUTHORS   Lee JH, Wang R, Xiong F, Krakowiak J, Liao Z, Nguyen PT, Moroz-Omori
            EV, Shao J, Zhu X, Bolt MJ, Wu H, Singh PK, Bi M, Shi CJ, Jamal N, 
            Li G, Mistry R, Jung SY, Tsai KL, Ferreon JC, Stossi F, Caflisch A, 
            Liu Z, Mancini MA, Li W
  TITLE     Enhancer RNA m6A methylation facilitates transcriptional condensate 
            formation and gene activation.
  JOURNAL   Mol Cell. 2021 Aug 19;81(16):3368-3385.e9. doi: 
            10.1016/j.molcel.2021.07.024. Epub 2021 Aug 9.
  PUBMED    34375583
REFERENCE   2  (bases 1 to 13430)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.07.024"; journalName: "Mol Cell"; date: 
            "2021-08-19- 19"; volume: "81"; issue: "16"; pages: "3368-3385.e9"
COMMENT     Sequence Label: pHR-YTHDC1-FL-mCherry-Cry2
FEATURES             Location/Qualifiers
     source          1..13430
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    43..160
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        313..720
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             3642..5135
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     misc_feature    5287..5875
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5340..5360)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5758..5769)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5878..5894)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5879..5895)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5985..6218
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6243..6261)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6244..6261)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6551..6570)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6784..6803)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6903..6925
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6963..6981)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7049..7153
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7154..7222,7223..8014)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7372..7391)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8185..8773
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8674..8693
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8927..8944
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9014..9034)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9019..9348
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9199..9334
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10482..10547
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10677..10697
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11122..11256
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11159..11178)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11213..11232
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             11424..12057
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    12104..12229
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12726..12959
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             13144..13188
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cggatctcga cggtatcgcc aaatggcagt attcatccac aattttaaaa gaaaaggggg
       61 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
      121 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
      181 cagagatcca gtttggatcg ataagcttga tatcgaattc ctgcagcccc gataaaataa
      241 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
      301 aagctagctg cagtaacgcc attttgcaag gcatggaaaa ataccaaacc aagaatagag
      361 aagttcagat caagggcggg tacatgaaaa tagctaacgt tgggccaaac aggatatctg
      421 cggtgagcag tttcggcccc ggcccggggc caagaacaga tggtcaccgc agtttcggcc
      481 ccggcccgag gccaagaaca gatggtcccc agatatggcc caaccctcag cagtttctta
      541 agacccatca gatgtttcca ggctccccca aggacctgaa atgaccctgc gccttatttg
      601 aattaaccaa tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat
      661 aaaagagctc acaacccctc actcggcgcg ccagtcctcc gacagactga gtcgcccggg
      721 ggggatccgg agctaccctg atggcggctg acagtcggga ggagaaagat ggagaactta
      781 atgttctgga tgatatttta actgaagtac cagaacaaga tgatgaactg tataatccag
      841 agagtgaaca agataaaaat gagaaaaagg gatcaaaaag aaaaagtgat cgaatggaat
      901 ctactgatac caaacgacaa aagccttctg tccattcaag acaactggtt tctaagccac
      961 tgagctcatc tgttagcaat aacaaaagaa tagttagtac aaaaggaaag tcagccacag
     1021 agtataaaaa tgaggaatat caaagatctg aaagaaacaa gcgtctagat gctgatcgga
     1081 aaattcgtct atcaagtagt gcctccagag aaccttataa gaatcaacct gaaaaaacct
     1141 gtgtccggaa aagggatcct gaaaggaggg ccaaatctcc tacgccagat ggttctgaga
     1201 gaattgggct tgaagtggat agacgtgcaa gcagatccag ccagtcttct aaggaagaag
     1261 tgaactctga agaatatggc tctgaccatg agactggcag cagtggttct tctgatgagc
     1321 aagggaacaa cactgagaat gaggaggaag gagtggaaga agatgtggag gaagatgaag
     1381 aagtagaaga agatgcagaa gaagatgaag aggtggatga agatggagag gaggaggagg
     1441 aagaggagga ggaggaagag gaggaggagg aggaggaaga agaagaatat gaacaggatg
     1501 agagagacca gaaagaggag ggaaatgatt atgacactcg aagtgaggcc agtgactctg
     1561 gttctgaatc tgtttccttc acagatgggt ctgtcagatc tggttcaggc acagatggat
     1621 cagatgagaa aaagaaggaa aggaagagag ctagaggcat atctccaatt gtttttgata
     1681 gaagtggaag ctctgcatca gagtcatatg caggttcaga aaagaagcat gagaaattat
     1741 catcttccgt tcgtgctgtc cgaaaagatc aaaccagtaa actcaaatat gtgcttcaag
     1801 atgcaagatt tttcctcata aagagtaaca accatgagaa tgtgtctctt gccaaagcga
     1861 agggtgtatg gtccacgctc cctgtaaatg agaagaaatt aaatcttgca tttagatctg
     1921 caaggagtgt tatcttaata ttttctgtca gagagagtgg aaaatttcaa gggtttgcaa
     1981 gactttcttc agaatcacat cacggaggat ctcctataca ctgggtgctt ccagcaggaa
     2041 tgagtgctaa aatgctggga ggtgtcttta aaattgactg gatttgcagg cgtgaattac
     2101 ccttcactaa gtcggctcat ctcaccaatc cttggaatga acataaacca gtaaagatcg
     2161 gacgtgatgg acaggaaatt gaacttgaat gtggaaccca gctttgtctt ctgtttcccc
     2221 ccgatgaaag tattgacttg tatcaggtca ttcataaaat gcgtcacaag agaagaatgc
     2281 attctcagcc ccgatcacga ggacgtccat cccgtcgaga accagtccgg gatgtgggaa
     2341 ggcgtcgacc agaagattat gatattcata acagcagaaa gaaaccaagg attgactatc
     2401 cccctgagtt tcaccagaga ccagggtatt taaaggatcc acgataccag gaagtggaca
     2461 gacgattttc aggagttcgc cgagatgtgt ttttaaatgg gtcctacaat gattatgtga
     2521 gggaatttca taacatggga ccaccaccac cttggcaagg aatgccccct tacccaggaa
     2581 tggaacaacc tccacaccat ccttactatc agcaccatgc tccacctcct caagctcatc
     2641 ccccttactc aggacatcat ccagtaccac atgaagcaag atacagagat aaacgagtac
     2701 atgattatga tatgagggtg gatgatttcc ttcgtcgcac acaagctgtt gtcagtggcc
     2761 ggagaagtag accccgtgaa agagaccggg aacgagagcg agaccgccct agagataaca
     2821 gacgagacag agagcgagat agaggacgtg atagagaaag agaaagagag cgattatgtg
     2881 atcgagacag agaccgaggg gagagaggtc gatatagaag aatggtgtct aaaggcgagg
     2941 aggataacat ggctataatc aaggaattta tgaggttcaa ggtgcatatg gagggatctg
     3001 tgaacggtca cgagttcgaa atcgagggcg aaggcgaggg gcgaccctat gaagggacac
     3061 agacggccaa actgaaggtg accaagggtg gccccctgcc tttcgcctgg gacatcctgt
     3121 caccccagtt tatgtacggc tctaaggctt acgttaagca ccctgccgat attccggact
     3181 acttgaaact gagcttccct gaagggttca agtgggaacg ggtaatgaat tttgaggatg
     3241 gcggtgtagt cacagttacc caggacagct ctcttcagga cggagaattt atctataagg
     3301 ttaaacttcg gggcactaac ttcccatcag acggccccgt gatgcagaag aaaactatgg
     3361 gctgggaagc cagctcagaa cgcatgtatc ccgaggacgg agcactgaag ggagaaatca
     3421 agcagcgact caagctgaag gatgggggac attatgatgc tgaggtcaaa accacctata
     3481 aggccaagaa acccgttcag cttcctgggg catacaacgt gaatatcaaa ctggatatta
     3541 catcccataa cgaagactat accatcgtgg aacagtacga gcgggccgag ggcagacata
     3601 gcacaggggg catggatgaa ttgtacaagg cggccacgcg tatgaagatg gacaaaaaga
     3661 ctatagtttg gtttagaaga gacctaagga ttgaggataa tcctgcatta gcagcagctg
     3721 ctcacgaagg atctgttttt cctgtcttca tttggtgtcc tgaagaagaa ggacagtttt
     3781 atcctggaag agcttcaaga tggtggatga aacaatcact tgctcactta tctcaatcct
     3841 tgaaggctct tggatctgac ctcactttaa tcaaaaccca caacacgatt tcagcgatct
     3901 tggattgtat ccgcgttacc ggtgctacaa aagtcgtctt taaccacctc tatgatcctg
     3961 tttcgttagt tcgggaccat accgtaaagg agaagctggt ggaacgtggg atctctgtgc
     4021 aaagctacaa tggagatcta ttgtatgaac cgtgggagat atactgcgaa aagggcaaac
     4081 cttttacgag tttcaattct tactggaaga aatgcttaga tatgtcgatt gaatccgtta
     4141 tgcttcctcc tccttggcgg ttgatgccaa taactgcagc ggctgaagcg atttgggcgt
     4201 gttcgattga agaactaggg ctggagaatg aggccgagaa accgagcaat gcgttgttaa
     4261 ctagagcttg gtctccagga tggagcaatg ctgataagtt actaaatgag ttcatcgaga
     4321 agcagttgat agattatgca aagaacagca agaaagttgt tgggaattct acttcactac
     4381 tttctccgta tctccatttc ggggaaataa gcgtcagaca cgttttccag tgtgcccgga
     4441 tgaaacaaat tatatgggca agagataaga acagtgaagg agaagaaagt gcagatcttt
     4501 ttcttagggg aatcggttta agagagtatt ctcggtatat atgtttcaac ttcccgttta
     4561 ctcacgagca atcgttgttg agtcatcttc ggtttttccc ttgggatgct gatgttgata
     4621 agttcaaggc ctggagacaa ggcaggaccg gttatccgtt ggtggatgcc ggaatgagag
     4681 agctttgggc taccggatgg atgcataaca gaataagagt gattgtttca agctttgctg
     4741 tgaagtttct tctccttcca tggaaatggg gaatgaagta tttctgggat acacttttgg
     4801 atgctgattt ggaatgtgac atccttggct ggcagtatat ctctgggagt atccccgatg
     4861 gccacgagct tgatcgcttg gacaatcccg cgttacaagg cgccaaatat gacccagaag
     4921 gtgagtacat aaggcaatgg cttcccgagc ttgcgagatt gccaactgaa tggatccatc
     4981 atccatggga cgctccttta accgtactca aagcttctgg tgtggaactc ggaacaaact
     5041 atgcgaaacc cattgtagac atcgacacag ctcgtgagct actagctaaa gctatttcaa
     5101 gaacccgtga agcacagatc atgatcggag cagcagcccg ggatccaccg gtcgccaccg
     5161 gttcagggag cggatccggc tcagggagtg cggccgcaac tcccacctgc aacatgcgtg
     5221 actgactgag gccgcgactc tagagtcgac ctgcaggcat gcaagcttga tatcaagctt
     5281 atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     5341 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     5401 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     5461 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     5521 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     5581 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     5641 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     5701 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     5761 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     5821 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga
     5881 taccgtcgac ctcgagggaa ttaattcgag ctcggtacct ttaagaccaa tgacttacaa
     5941 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     6001 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     6061 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     6121 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     6181 cctcagaccc ttttagtcag tgtggaaaat ctctagcagc atctagaatt aattccgtgt
     6241 attctatagt gtcacctaaa tcgtatgtgt atgatacata aggttatgta ttaattgtag
     6301 ccgcgttcta acgacaatat gtacaagcct aattgtgtag catctggctt actgaagcag
     6361 accctatcat ctctctcgta aactgccgtc agagtcggtt tggttggacg aaccttctga
     6421 gtttctggta acgccgtccc gcacccggaa atggtcagcg aaccaatcag cagggtcatc
     6481 gctagccaga tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg
     6541 gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg
     6601 ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg
     6661 ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta
     6721 ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgaat ggtgcactct
     6781 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     6841 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     6901 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     6961 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     7021 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     7081 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     7141 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     7201 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     7261 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     7321 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     7381 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     7441 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     7501 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     7561 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     7621 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     7681 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     7741 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     7801 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     7861 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     7921 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     7981 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     8041 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     8101 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     8161 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     8221 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     8281 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     8341 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     8401 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     8461 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     8521 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     8581 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     8641 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     8701 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     8761 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     8821 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     8881 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     8941 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     9001 ttaatgcagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     9061 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     9121 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     9181 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     9241 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     9301 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttggacac
     9361 aagacaggct tgcgagatat gtttgagaat accactttat cccgcgtcag ggagaggcag
     9421 tgcgtaaaaa gacgcggact catgtgaaat actggttttt agtgcgccag atctctataa
     9481 tctcgcgcaa cctattttcc cctcgaacac tttttaagcc gtagataaac aggctgggac
     9541 acttcacatg agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa
     9601 actcgcaagc cgactgatgc cttctgaaca atggaaaggc attattgccg taagccgtgg
     9661 cggtctgtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt
     9721 tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa
     9781 ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt
     9841 gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt
     9901 cgtccgctgg ttgatgacta tgttgttgat atcccgcaag atacctggat tgaacagccg
     9961 tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg
    10021 gcactgccgg gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat
    10081 tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt
    10141 taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt
    10201 gcagtcggcc cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat
    10261 gtggatctgg cgcggcattg acccacgcga aatcctcgac gtccaggcac gtattgtgat
    10321 gagcgatgcc gaacgtaccg acgatgattt atacgatacg gtgattggct accgtggcgg
    10381 caactggatt tatgagtggg ccccggatct ttgtgaagga accttacttc tgtggtgtga
    10441 cataattgga caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa
    10501 gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg
    10561 gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag
    10621 aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa
    10681 aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga
    10741 gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa
    10801 aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc
    10861 ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg
    10921 ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta
    10981 ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt
    11041 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
    11101 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
    11161 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
    11221 tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa
    11281 aatcatccca aacttcccac cccataccct attaccactg ccaattacct gtggtttcat
    11341 ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa
    11401 atatgtacta caaacttagt agttggaagg gctaattcac tcccaaagaa gacaagatat
    11461 ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga actacacacc
    11521 agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
    11581 gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag
    11641 cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct
    11701 agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg
    11761 agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac
    11821 tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt
    11881 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
    11941 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
    12001 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
    12061 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
    12121 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
    12181 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
    12241 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
    12301 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
    12361 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
    12421 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
    12481 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
    12541 caaaagtaag accaccgcac agcaagcggc cggccgctga tcttcagacc tggaggagga
    12601 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
    12661 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
    12721 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
    12781 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
    12841 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
    12901 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
    12961 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
    13021 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
    13081 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
    13141 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
    13201 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
    13261 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
    13321 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
    13381 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
//
