LOCUS       sequence-348712-       12350 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express IDR1 fragment of YTHDC1 with mCherry and CRY2 tag for 
            Optodroplet Assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12350)
  AUTHORS   Lee JH, Wang R, Xiong F, Krakowiak J, Liao Z, Nguyen PT, Moroz-Omori
            EV, Shao J, Zhu X, Bolt MJ, Wu H, Singh PK, Bi M, Shi CJ, Jamal N, 
            Li G, Mistry R, Jung SY, Tsai KL, Ferreon JC, Stossi F, Caflisch A, 
            Liu Z, Mancini MA, Li W
  TITLE     Enhancer RNA m6A methylation facilitates transcriptional condensate 
            formation and gene activation.
  JOURNAL   Mol Cell. 2021 Aug 19;81(16):3368-3385.e9. doi: 
            10.1016/j.molcel.2021.07.024. Epub 2021 Aug 9.
  PUBMED    34375583
REFERENCE   2  (bases 1 to 12350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.07.024"; journalName: "Mol Cell"; date: 
            "2021-08-19- 19"; volume: "81"; issue: "16"; pages: "3368-3385.e9"
COMMENT     Sequence Label: pHR-IDR1-mCherry-Cry2
FEATURES             Location/Qualifiers
     source          1..12350
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    43..160
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        313..720
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             2562..4055
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     misc_feature    4207..4795
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4260..4280)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4678..4689)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4798..4814)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4799..4815)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4905..5138
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5163..5181)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5164..5181)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5471..5490)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5704..5723)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5823..5845
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5883..5901)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5969..6073
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6074..6142,6143..6934)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRILYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6292..6311)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7105..7693
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7594..7613
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7847..7864
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7934..7954)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7939..8268
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8119..8254
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9402..9467
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9597..9617
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10042..10176
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10079..10098)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10133..10152
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10344..10977
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11024..11149
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11646..11879
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12064..12108
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cggatctcga cggtatcgcc aaatggcagt attcatccac aattttaaaa gaaaaggggg
       61 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
      121 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
      181 cagagatcca gtttggatcg ataagcttga tatcgaattc ctgcagcccc gataaaataa
      241 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
      301 aagctagctg cagtaacgcc attttgcaag gcatggaaaa ataccaaacc aagaatagag
      361 aagttcagat caagggcggg tacatgaaaa tagctaacgt tgggccaaac aggatatctg
      421 cggtgagcag tttcggcccc ggcccggggc caagaacaga tggtcaccgc agtttcggcc
      481 ccggcccgag gccaagaaca gatggtcccc agatatggcc caaccctcag cagtttctta
      541 agacccatca gatgtttcca ggctccccca aggacctgaa atgaccctgc gccttatttg
      601 aattaaccaa tcagcctgct tctcgcttct gttcgcgcgc ttctgcttcc cgagctctat
      661 aaaagagctc acaacccctc actcggcgcg ccagtcctcc gacagactga gtcgcccggg
      721 ggggatctgg agctctcgag aattctcacg cgtcaagtgg agcaaggcag gtggacagtg
      781 gatccggagc taccctgatg gcggctgaca gtcgggagga gaaagatgga gaacttaatg
      841 ttctggatga tattttaact gaagtaccag aacaagatga tgaactgtat aatccagaga
      901 gtgaacaaga taaaaatgag aaaaagggat caaaaagaaa aagtgatcga atggaatcta
      961 ctgataccaa acgacaaaag ccttctgtcc attcaagaca actggtttct aagccactga
     1021 gctcatctgt tagcaataac aaaagaatag ttagtacaaa aggaaagtca gccacagagt
     1081 ataaaaatga ggaatatcaa agatctgaaa gaaacaagcg tctagatgct gatcggaaaa
     1141 ttcgtctatc aagtagtgcc tccagagaac cttataagaa tcaacctgaa aaaacctgtg
     1201 tccggaaaag ggatcctgaa aggagggcca aatctcctac gccagatggt tctgagagaa
     1261 ttgggcttga agtggataga cgtgcaagca gatccagcca gtcttctaag gaagaagtga
     1321 actctgaaga atatggctct gaccatgaga ctggcagcag tggttcttct gatgagcaag
     1381 ggaacaacac tgagaatgag gaggaaggag tggaagaaga tgtggaggaa gatgaagaag
     1441 tagaagaaga tgcagaagaa gatgaagagg tggatgaaga tggagaggag gaggaggaag
     1501 aggaggagga ggaagaggag gaggaggagg aggaagaaga agaatatgaa caggatgaga
     1561 gagaccagaa agaggaggga aatgattatg acactcgaag tgaggccagt gactctggtt
     1621 ctgaatctgt ttccttcaca gatgggtctg tcagatctgg ttcaggcaca gatggatcag
     1681 atgagaaaaa gaaggaaagg aagagagcta gaggcatatc tccaattgtt tttgatagaa
     1741 gtggaagctc tgcatcagag tcatatgcag gttcagaaaa gaagcatgag aaattatcat
     1801 cttccgttcg tgctgtccga aaagatcaaa ccagtaaact catggtgtct aaaggcgagg
     1861 aggataacat ggctataatc aaggaattta tgaggttcaa ggtgcatatg gagggatctg
     1921 tgaacggtca cgagttcgaa atcgagggcg aaggcgaggg gcgaccctat gaagggacac
     1981 agacggccaa actgaaggtg accaagggtg gccccctgcc tttcgcctgg gacatcctgt
     2041 caccccagtt tatgtacggc tctaaggctt acgttaagca ccctgccgat attccggact
     2101 acttgaaact gagcttccct gaagggttca agtgggaacg ggtaatgaat tttgaggatg
     2161 gcggtgtagt cacagttacc caggacagct ctcttcagga cggagaattt atctataagg
     2221 ttaaacttcg gggcactaac ttcccatcag acggccccgt gatgcagaag aaaactatgg
     2281 gctgggaagc cagctcagaa cgcatgtatc ccgaggacgg agcactgaag ggagaaatca
     2341 agcagcgact caagctgaag gatgggggac attatgatgc tgaggtcaaa accacctata
     2401 aggccaagaa acccgttcag cttcctgggg catacaacgt gaatatcaaa ctggatatta
     2461 catcccataa cgaagactat accatcgtgg aacagtacga gcgggccgag ggcagacata
     2521 gcacaggggg catggatgaa ttgtacaagg cggccacgcg tatgaagatg gacaaaaaga
     2581 ctatagtttg gtttagaaga gacctaagga ttgaggataa tcctgcatta gcagcagctg
     2641 ctcacgaagg atctgttttt cctgtcttca tttggtgtcc tgaagaagaa ggacagtttt
     2701 atcctggaag agcttcaaga tggtggatga aacaatcact tgctcactta tctcaatcct
     2761 tgaaggctct tggatctgac ctcactttaa tcaaaaccca caacacgatt tcagcgatct
     2821 tggattgtat ccgcgttacc ggtgctacaa aagtcgtctt taaccacctc tatgatcctg
     2881 tttcgttagt tcgggaccat accgtaaagg agaagctggt ggaacgtggg atctctgtgc
     2941 aaagctacaa tggagatcta ttgtatgaac cgtgggagat atactgcgaa aagggcaaac
     3001 cttttacgag tttcaattct tactggaaga aatgcttaga tatgtcgatt gaatccgtta
     3061 tgcttcctcc tccttggcgg ttgatgccaa taactgcagc ggctgaagcg atttgggcgt
     3121 gttcgattga agaactaggg ctggagaatg aggccgagaa accgagcaat gcgttgttaa
     3181 ctagagcttg gtctccagga tggagcaatg ctgataagtt actaaatgag ttcatcgaga
     3241 agcagttgat agattatgca aagaacagca agaaagttgt tgggaattct acttcactac
     3301 tttctccgta tctccatttc ggggaaataa gcgtcagaca cgttttccag tgtgcccgga
     3361 tgaaacaaat tatatgggca agagataaga acagtgaagg agaagaaagt gcagatcttt
     3421 ttcttagggg aatcggttta agagagtatt ctcggtatat atgtttcaac ttcccgttta
     3481 ctcacgagca atcgttgttg agtcatcttc ggtttttccc ttgggatgct gatgttgata
     3541 agttcaaggc ctggagacaa ggcaggaccg gttatccgtt ggtggatgcc ggaatgagag
     3601 agctttgggc taccggatgg atgcataaca gaataagagt gattgtttca agctttgctg
     3661 tgaagtttct tctccttcca tggaaatggg gaatgaagta tttctgggat acacttttgg
     3721 atgctgattt ggaatgtgac atccttggct ggcagtatat ctctgggagt atccccgatg
     3781 gccacgagct tgatcgcttg gacaatcccg cgttacaagg cgccaaatat gacccagaag
     3841 gtgagtacat aaggcaatgg cttcccgagc ttgcgagatt gccaactgaa tggatccatc
     3901 atccatggga cgctccttta accgtactca aagcttctgg tgtggaactc ggaacaaact
     3961 atgcgaaacc cattgtagac atcgacacag ctcgtgagct actagctaaa gctatttcaa
     4021 gaacccgtga agcacagatc atgatcggag cagcagcccg ggatccaccg gtcgccaccg
     4081 gttcagggag cggatccggc tcagggagtg cggccgcaac tcccacctgc aacatgcgtg
     4141 actgactgag gccgcgactc tagagtcgac ctgcaggcat gcaagcttga tatcaagctt
     4201 atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     4261 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     4321 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     4381 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     4441 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     4501 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     4561 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     4621 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     4681 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     4741 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga
     4801 taccgtcgac ctcgagggaa ttaattcgag ctcggtacct ttaagaccaa tgacttacaa
     4861 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     4921 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     4981 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     5041 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     5101 cctcagaccc ttttagtcag tgtggaaaat ctctagcagc atctagaatt aattccgtgt
     5161 attctatagt gtcacctaaa tcgtatgtgt atgatacata aggttatgta ttaattgtag
     5221 ccgcgttcta acgacaatat gtacaagcct aattgtgtag catctggctt actgaagcag
     5281 accctatcat ctctctcgta aactgccgtc agagtcggtt tggttggacg aaccttctga
     5341 gtttctggta acgccgtccc gcacccggaa atggtcagcg aaccaatcag cagggtcatc
     5401 gctagccaga tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg
     5461 gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg
     5521 ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg
     5581 ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta
     5641 ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgaat ggtgcactct
     5701 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     5761 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     5821 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     5881 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     5941 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     6001 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     6061 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     6121 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     6181 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     6241 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     6301 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tactctattc
     6361 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     6421 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     6481 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     6541 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     6601 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     6661 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     6721 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     6781 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     6841 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     6901 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     6961 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     7021 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     7081 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     7141 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     7201 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     7261 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     7321 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     7381 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     7441 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     7501 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     7561 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     7621 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     7681 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     7741 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     7801 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     7861 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     7921 ttaatgcagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     7981 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     8041 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     8101 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     8161 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     8221 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttggacac
     8281 aagacaggct tgcgagatat gtttgagaat accactttat cccgcgtcag ggagaggcag
     8341 tgcgtaaaaa gacgcggact catgtgaaat actggttttt agtgcgccag atctctataa
     8401 tctcgcgcaa cctattttcc cctcgaacac tttttaagcc gtagataaac aggctgggac
     8461 acttcacatg agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa
     8521 actcgcaagc cgactgatgc cttctgaaca atggaaaggc attattgccg taagccgtgg
     8581 cggtctgtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt
     8641 tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa
     8701 ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt
     8761 gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt
     8821 cgtccgctgg ttgatgacta tgttgttgat atcccgcaag atacctggat tgaacagccg
     8881 tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg
     8941 gcactgccgg gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat
     9001 tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt
     9061 taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt
     9121 gcagtcggcc cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat
     9181 gtggatctgg cgcggcattg acccacgcga aatcctcgac gtccaggcac gtattgtgat
     9241 gagcgatgcc gaacgtaccg acgatgattt atacgatacg gtgattggct accgtggcgg
     9301 caactggatt tatgagtggg ccccggatct ttgtgaagga accttacttc tgtggtgtga
     9361 cataattgga caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa
     9421 gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg
     9481 gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag
     9541 aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa
     9601 aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga
     9661 gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa
     9721 aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc
     9781 ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg
     9841 ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta
     9901 ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt
     9961 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
    10021 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
    10081 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
    10141 tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa
    10201 aatcatccca aacttcccac cccataccct attaccactg ccaattacct gtggtttcat
    10261 ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa
    10321 atatgtacta caaacttagt agttggaagg gctaattcac tcccaaagaa gacaagatat
    10381 ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga actacacacc
    10441 agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
    10501 gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag
    10561 cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct
    10621 agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg
    10681 agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac
    10741 tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt
    10801 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
    10861 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
    10921 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
    10981 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
    11041 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
    11101 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
    11161 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
    11221 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
    11281 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
    11341 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
    11401 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
    11461 caaaagtaag accaccgcac agcaagcggc cggccgctga tcttcagacc tggaggagga
    11521 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
    11581 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
    11641 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
    11701 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
    11761 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
    11821 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
    11881 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
    11941 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
    12001 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
    12061 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
    12121 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
    12181 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
    12241 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
    12301 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
//
