LOCUS       sequence-348714-       14261 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dCasRx mediated RNA tethering of FTO.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14261)
  AUTHORS   Lee JH, Wang R, Xiong F, Krakowiak J, Liao Z, Nguyen PT, Moroz-Omori
            EV, Shao J, Zhu X, Bolt MJ, Wu H, Singh PK, Bi M, Shi CJ, Jamal N, 
            Li G, Mistry R, Jung SY, Tsai KL, Ferreon JC, Stossi F, Caflisch A, 
            Liu Z, Mancini MA, Li W
  TITLE     Enhancer RNA m6A methylation facilitates transcriptional condensate 
            formation and gene activation.
  JOURNAL   Mol Cell. 2021 Aug 19;81(16):3368-3385.e9. doi: 
            10.1016/j.molcel.2021.07.024. Epub 2021 Aug 9.
  PUBMED    34375583
REFERENCE   2  (bases 1 to 14261)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.07.024"; journalName: "Mol Cell"; date: 
            "2021-08-19- 19"; volume: "81"; issue: "16"; pages: "3368-3385.e9"
COMMENT     Sequence Label: plenti-ef1a-dCasRx-FTO-HA-T2A-BSD
FEATURES             Location/Qualifiers
     source          1..14261
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2687..3865
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2918..3856
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3813..3833
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3884..3904
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3914..6811
                     /codon_start=1
                     /product="RNA-targeting Type VI CRISPR protein from 
                     Ruminococcus flavefaciens strain XPD3002 (Konermann et al.,
                     2018)"
                     /label=RfxCas13d
                     /translation="IEKKKSFAKGMGVKSTLVSGSKVYMTTFAEGSDARLEKIVEGDSI
                     RSVNEGEAFSAEMADKNAGYKIGNAKFSHPKGYAVVANNPLYTGPVQQDMLGLKETLEK
                     RYFGESADGNDNICIQVIHNILDIEKILAEYITNAAYAVNNISGLDKDIIGFGKFSTVY
                     TYDEFKDPEHHRAAFNNNDKLINAIKAQYDEFDNFLDNPRLGYFGQAFFSKEGRNYIIN
                     YGNECYDILALLSGLAHWVVANNEEESRISRTWLYNLDKNLDNEYISTLNYLYDRITNE
                     LTNSFSKNSAANVNYIAETLGINPAEFAEQYFRFSIMKEQKNLGFNITKLREVMLDRKD
                     MSEIRKNHKVFDSIRTKVYTMMDFVIYRYYIEEDAKVAAANKSLPDNEKSLSEKDIFVI
                     NLRGSFNDDQKDALYYDEANRIWRKLENIMHNIKEFRGNKTREYKKKDAPRLPRILPAG
                     RDVSAFSKLMYALTMFLDGKEINDLLTTLINKFDNIQSFLKVMPLIGVNAKFVEEYAFF
                     KDSAKIADELRLIKSFARMGEPIADARRAMYIDAIRILGTNLSYDELKALADTFSLDEN
                     GNKLKKGKHGMRNFIINNVISNKRFHYLIRYGDPAHLHEIAKNEAVVKFVLGRIADIQK
                     KQGQNGKNQIDRYYETCIGKDKGKSVSEKVDALTKIITGMNYDQFDKKRSVIEDTGREN
                     AEREKFKKIISLYLTVIYHILKNIVNINARYVIGFHCVERDAQLYKEKGYDINLKKLEE
                     KGFSSVTKLCAGIDETAPDKRKDVEKEMAERAKESIDSLESANPKLYANYIKYSDEKKA
                     EEFTRQINREKAKTALNAYLRNTKWNVIIREDLLRIDNKTCTLFANKAVALEVARYVHA
                     YINDIAEVNSYFQLYHYIMQRIIMNERYEKSSGKVSEYFDAVNDEKKYNDRLLKLLCVP
                     FGYCIPRFKNLSIEALFDRNEAAKFDKEKKKVSGNS"
     CDS             6863..6883
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8426..8452
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             8468..8521
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             8522..8920
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8945..9533
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8998..9018)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9416..9427)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9536..9552)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9537..9553)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10066..10246
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10272..10289)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10278..10502
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10548..10976
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10635..10654)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10845..10866
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10985..11005)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10990..11319
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11170..11305
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11232..11251
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        11367..11414
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             11433..11807
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11937..12058
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11974..11993)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12028..12047
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12107..12123)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12107..12123)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12120..12142)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12131..12147
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12155..12161,12162..12179,12180..12185))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12200..12221
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(12509..13097)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12589..12608)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13268..14059,14060..14128))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13891..13910
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14129..14233)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta gctagctgca aagatggata aagttttaaa cagagaggaa
     2641 tctttgcagc taatggacct tctaggtctt gaaaggagtg ggaattggct ccggtgcccg
     2701 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
     2761 ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
     2821 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
     2881 cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg
     2941 cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct
     3001 gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc
     3061 ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg
     3121 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct
     3181 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt
     3241 aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg
     3301 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag
     3361 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc
     3421 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga
     3481 aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg
     3541 agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc
     3601 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt
     3661 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac
     3721 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt
     3781 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt
     3841 ttttcttcca tttcaggtgt cgtgacgtac ggccaccatg agccccaaga agaagagaaa
     3901 ggtggaggcc agcatcgaaa aaaaaaagtc cttcgccaag ggcatgggcg tgaagtccac
     3961 actcgtgtcc ggctccaaag tgtacatgac aaccttcgcc gaaggcagcg acgccaggct
     4021 ggaaaagatc gtggagggcg acagcatcag gagcgtgaat gagggcgagg ccttcagcgc
     4081 tgaaatggcc gataaaaacg ccggctataa gatcggcaac gccaaattca gccatcctaa
     4141 gggctacgcc gtggtggcta acaaccctct gtatacagga cccgtccagc aggatatgct
     4201 cggcctgaag gaaactctgg aaaagaggta cttcggcgag agcgctgatg gcaatgacaa
     4261 tatttgtatc caggtgatcc ataacatcct ggacattgaa aaaatcctcg ccgaatacat
     4321 taccaacgcc gcctacgccg tcaacaatat ctccggcctg gataaggaca ttattggatt
     4381 cggcaagttc tccacagtgt atacctacga cgaattcaaa gaccccgagc accatagggc
     4441 cgctttcaac aataacgata agctcatcaa cgccatcaag gcccagtatg acgagttcga
     4501 caacttcctc gataacccca gactcggcta tttcggccag gcctttttca gcaaggaggg
     4561 cagaaattac atcatcaatt acggcaacga atgctatgac attctggccc tcctgagcgg
     4621 actggcgcac tgggtggtcg ctaacaacga agaagagtcc aggatctcca ggacctggct
     4681 ctacaacctc gataagaacc tcgacaacga atacatctcc accctcaact acctctacga
     4741 caggatcacc aatgagctga ccaactcctt ctccaagaac tccgccgcca acgtgaacta
     4801 tattgccgaa actctgggaa tcaaccctgc cgaattcgcc gaacaatatt tcagattcag
     4861 cattatgaaa gagcagaaaa acctcggatt caatatcacc aagctcaggg aagtgatgct
     4921 ggacaggaag gatatgtccg agatcaggaa aaatcataag gtgttcgact ccatcaggac
     4981 caaggtctac accatgatgg actttgtgat ttataggtat tacatcgaag aggatgccaa
     5041 ggtggctgcc gccaataagt ccctccccga taatgagaag tccctgagcg agaaggatat
     5101 ctttgtgatt aacctgaggg gctccttcaa cgacgaccag aaggatgccc tctactacga
     5161 tgaagctaat agaatttgga gaaagctcga aaatatcatg cacaacatca aggaatttag
     5221 gggaaacaag acaagagagt ataagaagaa ggacgcccct agactgccca gaatcctgcc
     5281 cgctggccgt gatgtttccg ccttcagcaa actcatgtat gccctgacca tgttcctgga
     5341 tggcaaggag atcaacgacc tcctgaccac cctgattaat aaattcgata acatccagag
     5401 cttcctgaag gtgatgcctc tcatcggagt caacgctaag ttcgtggagg aatacgcctt
     5461 tttcaaagac tccgccaaga tcgccgatga gctgaggctg atcaagtcct tcgctagaat
     5521 gggagaacct attgccgatg ccaggagggc catgtatatc gacgccatcc gtattttagg
     5581 aaccaacctg tcctatgatg agctcaaggc cctcgccgac accttttccc tggacgagaa
     5641 cggaaacaag ctcaagaaag gcaagcacgg catgagaaat ttcattatta ataacgtgat
     5701 cagcaataaa aggttccact acctgatcag atacggtgat cctgcccacc tccatgagat
     5761 cgccaaaaac gaggccgtgg tgaagttcgt gctcggcagg atcgctgaca tccagaaaaa
     5821 acagggccag aacggcaaga accagatcga caggtactac gaaacttgta tcggaaagga
     5881 taagggcaag agcgtgagcg aaaaggtgga cgctctcaca aagatcatca ccggaatgaa
     5941 ctacgaccaa ttcgacaaga aaaggagcgt cattgaggac accggcaggg aaaacgccga
     6001 gagggagaag tttaaaaaga tcatcagcct gtacctcacc gtgatctacc acatcctcaa
     6061 gaatattgtc aatatcaacg ccaggtacgt catcggattc cattgcgtcg agcgtgatgc
     6121 tcaactgtac aaggagaaag gctacgacat caatctcaag aaactggaag agaagggatt
     6181 cagctccgtc accaagctct gcgctggcat tgatgaaact gcccccgata agagaaagga
     6241 cgtggaaaag gagatggctg aaagagccaa ggagagcatt gacagcctcg agagcgccaa
     6301 ccccaagctg tatgccaatt acatcaaata cagcgacgag aagaaagccg aggagttcac
     6361 caggcagatt aacagggaga aggccaaaac cgccctgaac gcctacctga ggaacaccaa
     6421 gtggaatgtg atcatcaggg aggacctcct gagaattgac aacaagacat gtaccctgtt
     6481 cgcaaacaag gccgtcgccc tggaagtggc caggtatgtc cacgcctata tcaacgacat
     6541 tgccgaggtc aattcctact tccaactgta ccattacatc atgcagagaa ttatcatgaa
     6601 tgagaggtac gagaaaagca gcggaaaggt gtccgagtac ttcgacgctg tgaatgacga
     6661 gaagaagtac aacgataggc tcctgaaact gctgtgtgtg cctttcggct actgtatccc
     6721 caggtttaag aacctgagca tcgaggccct gttcgatagg aacgaggccg ccaagttcga
     6781 caaggagaaa aagaaggtgt ccggcaatag cgctggagga ggtggaagcg gaggaggagg
     6841 aagcggagga ggaggtagcg gacctaagaa aaagaggaag gtggcggccg ctggatccgg
     6901 tggcagcaag cgcaccccga ctgccgagga acgagagcgc gaagctaaga aactgaggct
     6961 tcttgaagag cttgaagaca cttggctccc ttatctgacc cccaaagatg atgaattcta
     7021 tcagcagtgg cagctgaaat atcctaaact aattctccga gaagccagca gtgtatctga
     7081 ggagctccat aaagaggttc aagaagcctt tctcacactg cacaagcatg gctgcttatt
     7141 tcgggacctg gttaggatcc aaggcaaaga tctgctcact ccggtatctc gcatcctcat
     7201 tggtaatcca ggctgcacct acaagtacct gaacaccagg ctctttacgg tcccctggcc
     7261 agtgaaaggg tctaatataa aacacaccga ggctgaaata gccgctgctt gtgagacctt
     7321 cctcaagctc aatgactacc tgcagataga aaccatccag gctttggaag aacttgctgc
     7381 caaagagaag gctaatgagg atgctgtgcc attgtgtatg tctgcagatt tccccagggt
     7441 tgggatgggt tcatcctaca acggacaaga tgaagtggac attaagagca gagcagcata
     7501 caacgtaact ttgctgaatt tcatggatcc tcagaaaatg ccatacctga aagaggaacc
     7561 ttattttggc atggggaaaa tggcagtgag ctggcatcat gatgaaaatc tggtggacag
     7621 gtcagcggtg gcagtgtaca gttatagctg tgaaggccct gaagaggaaa gtgaggatga
     7681 ctctcatctc gaaggcaggg atcctgatat ttggcatgtt ggttttaaga tctcatggga
     7741 catagagaca cctggtttgg cgatacccct tcaccaagga gactgctatt tcatgcttga
     7801 tgatctcaat gccacccacc aacactgtgt tttggccggt tcacaacctc ggtttagttc
     7861 cacccaccga gtggcagagt gctcaacagg aaccttggat tatattttac aacgctgtca
     7921 gttggctctg cagaatgtct gtgacgatgt ggacaatgat gatgtctctt tgaaatcctt
     7981 tgagcctgca gttttgaaac aaggagaaga aattcataat gaggtcgagt ttgagtggct
     8041 gaggcagttt tggtttcaag gcaatcgata cagaaagtgc actgactggt ggtgtcaacc
     8101 catggctcaa ctggaagcac tgtggaagaa gatggagggt gtgacaaatg ctgtgcttca
     8161 tgaagttaaa agagaggggc tccccgtgga acaaaggaat gaaatcttga ctgccatcct
     8221 tgcctcgctc actgcacgcc agaacctgag gagagaatgg catgccaggt gccagtcacg
     8281 aattgcccga acattacctg ctgatcagaa gccagaatgt cggccatact gggaaaagga
     8341 tgatgcttcg atgcctctgc cgtttgacct cacagacatc gtttcagaac tcagaggtca
     8401 gcttctggaa gcaaaacccg gtacctatcc atatgatgtt ccagattatg cttgtacagg
     8461 cagtggagag ggcagaggaa gtctgctaac atgcggtgac gtcgaggaga atcctggccc
     8521 aatggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa cggctacaat
     8581 caacagcatc cccatctctg aagactacag cgtcgccagc gcagctctct ctagcgacgg
     8641 ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg cagaactcgt
     8701 ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg
     8761 aaatgagaac aggggcatct tgagcccctg cggacggtgc cgacaggtgc ttctcgatct
     8821 gcatcctggg atcaaagcca tagtgaagga cagtgatgga cagccgacgg cagttgggat
     8881 tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa gaattcgata tcaagcttat
     8941 cggtaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     9001 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     9061 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     9121 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     9181 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     9241 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     9301 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     9361 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     9421 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     9481 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata
     9541 ccgtcgacct cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac
     9601 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac
     9661 acctcaggta cttaattaac tttaagacca atgacttaca aggcagctgt agatcttagc
     9721 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     9781 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca
     9841 ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     9901 gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg
     9961 agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc
    10021 ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta
    10081 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
    10141 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
    10201 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac
    10261 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
    10321 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
    10381 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
    10441 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
    10501 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
    10561 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
    10621 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
    10681 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
    10741 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
    10801 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
    10861 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
    10921 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
    10981 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
    11041 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
    11101 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
    11161 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
    11221 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
    11281 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
    11341 tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca
    11401 tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg
    11461 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct
    11521 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
    11581 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
    11641 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
    11701 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
    11761 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag
    11821 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
    11881 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
    11941 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
    12001 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
    12061 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
    12121 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
    12181 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
    12241 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
    12301 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
    12361 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
    12421 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
    12481 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
    12541 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
    12601 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
    12661 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
    12721 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
    12781 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
    12841 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
    12901 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
    12961 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
    13021 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
    13081 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
    13141 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
    13201 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
    13261 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
    13321 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
    13381 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
    13441 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
    13501 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
    13561 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
    13621 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
    13681 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
    13741 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    13801 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    13861 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
    13921 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    13981 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    14041 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    14101 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    14161 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    14221 taggggttcc gcgcacattt ccccgaaaag tgccacctga c
//
