LOCUS       sequence-348717-        2457 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses paxillin fused to blue fluorescent protein Electra1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Papadaki S, Wang X, Wang Y, Zhang H, Jia S, Liu S, Yang M, Zhang D, 
            Jia JM, Koster RW, Namikawa K, Piatkevich KD
  TITLE     Dual-expression system for blue fluorescent protein optimization.
  JOURNAL   Sci Rep. 2022 Jun 17;12(1):10190. doi: 10.1038/s41598-022-13214-0.
  PUBMED    35715437
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-06-17"; volume: "12(1):10190. doi"; pages: " 
            10.1038/s41598-022-13214-0"
COMMENT     Sequence Label: pPaxillin-Electra1-N1
FEATURES             Location/Qualifiers
     source          1..2457
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    1678..1730
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      1738..1747
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggacgacc tcgatgcctt actggcagac ctggaatcca ccacctcgca tatctctaag
       61 cgaccggtgt ttctaacaga ggaaacgcct tactcctacc caactggaaa ccatacgtac
      121 caggagattg ctgtgccacc gccggtgccc ccgccgcctt ccagtgaggc cctaaatggc
      181 actgtgattg accccttaga ccagtggcaa cccagcgtgt ccagatacgg ccaccagcaa
      241 cctcagtccc agtctccgat atacagctct agtgccaaaa gctccagtgc ctctgttcct
      301 agagacgggc tcagctcccc ttctccccgt gccagtgagg aggaacacgt gtacagcttc
      361 ccaaacaagc agaagtctgc agaaccatct cccacaatga ccagcacctc cctgggcagc
      421 aacctctcgg aactggacag gcttctcctg gaactgaatg ctgttcaaca taatcccccc
      481 agtggcttct cagcagatga agtcagcaga agcccatcac tgcccaatgt gaccggacct
      541 cactatgtca tcccagagag cagcagctct gcaggaggga aggctgcacc ccctacaaaa
      601 gaaaaaccaa agcggaatgg tgggcgtggg attgaagatg tgcgtcccag tgtggagagc
      661 ctgctggatg agctggagag ctctgtgcca agcccagtcc ctgcaatcac tgtgagccaa
      721 ggggaggtga gcagccccca gcgtgtcaac gccagtcagc agcagacccg tatctctgct
      781 tcttcagcta cacgagaact ggatgagctg atggcgtccc tctctgactt taagttcatg
      841 gcacagggga aagccggcgg cagcagctcc ccaccatcca ccaccccaaa gcctggcagt
      901 cagctggaca ccatgctggg aagtctgcag tctgacctga acaaactggg ggtagcgacg
      961 gtcgccaagg gtgtgtgcgg agcctgcaag aagccgattg ctgggcaggt agttacagcc
     1021 atggggaaga cctggcaccc tgagcacttc gtctgcaccc actgccagga ggagattgga
     1081 tcacggaact tctttgagcg ggatgggcag ccctactgtg agaaggacta tcacaacctc
     1141 ttctctcctc gctgctacta ctgcaatggg ccaatccttg ataaagtggt gacggctttg
     1201 gacaggacgt ggcaccctga acactttttc tgtgcccagt gtggagtttt ctttggacct
     1261 gaaggatttc atgagaagga tggcaaagcc tattgccgca aggactactt tgacatgttt
     1321 gctcccaagt gtggaggctg tgcccgggct atcctggaaa attacatttc tgccttaaac
     1381 accctgtggc accctgagtg ctttgtctgt cgggaatgtt tcacaccgtt catcaacggc
     1441 agcttcttcg agcacgacgg gcagccctac tgcgaggtgc attaccacga gcgccgcggc
     1501 tcgctgtgct ctggctgcca gaagcccatc acgggacgct gcatcactgc catgggcaag
     1561 aaatttcacc ccgaacactt tgtctgtgcc ttctgcctca agcagctcaa caaaggaacc
     1621 ttcaaagaac agaacgacaa gccctactgt cagaactgct ttctcaaact cttctgtctc
     1681 gagctcaagc ttcgaattct gcagtcgacg gtaccgcggg cccgggatcc accggtcgcc
     1741 accatggtgt ctaagggcga agagctgatc gaggaaaata tgcgtatgaa ggtggtcatg
     1801 gaaggttcgg tcaacggcca ccaattcaaa tgcacaggtg aaggagaagg cagaccgtac
     1861 gagggagtgc aaaccatgag gatcaaagtc atcgagggag gacccctgcc atttgccttt
     1921 gacattcttg ccacgtcgtt cctgttcggc agcaagacct ttatcaagta cccggccgac
     1981 atccctgatt tcttcaaaca gtcctttcct gagggcttta cttgggaaag agttacgaga
     2041 tacgaagatg gtggagtcgt caccgtcacg caggacacca gccttgagga tggggggctc
     2101 gtctacaacg tcaaggtcag aggggtaaac tttccctcca atggtcccgt gatgcagaag
     2161 aagaccgagg gttgggagcc tttcacagag atgatgtatc cagcaaatgg tggtctgaga
     2221 ggatataccg acatcgcact gaaagttgat ggtgatggcc atctgcacgc caacatcgtg
     2281 acaacttaca ggtcaaaaaa gaccgtcggg gacatcaaga tgcccggtgt ccatgccgtt
     2341 gattaccgcc tggaaagggt cgaagagagt gacaatgaaa cctacgtagt gctaagagaa
     2401 gtggcagttg ccaaatacag caaccttggt ggtggcatgg acgagctgta caagtaa
//
