LOCUS       sequence-348723-        1413 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of engineered CBX1 with enhanced binding to 
            H3K9me.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Albanese KI, Krone MW, Petell CJ, Parker MM, Strahl BD, Brustad EM, 
            Waters ML
  TITLE     Engineered Reader Proteins for Enhanced Detection of Methylated 
            Lysine on Histones.
  JOURNAL   ACS Chem Biol. 2020 Jan 17;15(1):103-111. doi: 
            10.1021/acschembio.9b00651. Epub 2019 Nov 1.
  PUBMED    31634430
REFERENCE   2  (bases 1 to 1413)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acschembio.9b00651"; journalName: "ACS Chem Biol"; date: 
            "2020-01-17- 17"; volume: "15"; issue: "1"; pages: "103-111"
COMMENT     Sequence Label: pET28a-MBP-CBX1-eReader
FEATURES             Location/Qualifiers
     source          1..1413
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..57
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             73..1170
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="KIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEE
                     KFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLI
                     AYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAA
                     DGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGET
                     AMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLE
                     NYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFW
                     YAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      1027..1036
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1144..1167
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1186..1206
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1393..1410
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat
       61 atggctagcg gtaaaatcga agaaggtaaa ctggtaatct ggattaacgg cgataaaggc
      121 tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag ataccggaat taaagtcacc
      181 gttgagcatc cggataaact ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc
      241 cctgacatta tcttctgggc acacgaccgc tttggtggct acgctcaatc tggcctgttg
      301 gctgaaatca ccccggacaa agcgttccag gacaagctgt atccgtttac ctgggatgcc
      361 gtacgttaca acggcaagct gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt
      421 tataacaaag atctgctgcc gaacccgcca aaaacctggg aagagatccc ggcgctggat
      481 aaagaactga aagcgaaagg taagagcgcg ctgatgttca acctgcaaga accgtacttc
      541 acctggccgc tgattgctgc tgacgggggt tatgcgttca agtatgaaaa cggcaagtac
      601 gacattaaag acgtgggcgt ggataacgct ggcgcgaaag cgggtctgac cttcctggtt
      661 gacctgatta aaaacaaaca catgaatgca gacaccgatt actccatcgc agaagctgcc
      721 tttaataaag gcgaaacagc gatgaccatc aacggcccgt gggcatggtc caacatcgac
      781 accagcaaag tgaattatgg tgtaacggta ctgccgacct tcaagggtca accatccaaa
      841 ccgttcgttg gcgtgctgag cgcaggtatt aacgccgcca gtccgaacaa agagctggcg
      901 aaagagttcc tcgaaaacta tctgctgact gatgaaggtc tggaagcggt taataaagac
      961 aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag agttggcgaa agatccacgt
     1021 attgccgcca ccatggaaaa cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg
     1081 tccgctttct ggtatgccgt gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact
     1141 gtcgatgaag ccctgaaaga cgcgcagact cgtatcacca agggtgaaaa tctatacttc
     1201 caaggatccg aagaagagga agaggaatat gtggtggaaa aagttcttga tcggcgagtt
     1261 gtcaagggca aggtggaata tcttctaaag tgggccggtt tctcagatga ggacaacact
     1321 tgggagccag aagagaatct gttttgccct gaccttattg ctgagtttct acagtcacag
     1381 aaaacactcg agcaccacca ccaccaccac tga
//
