LOCUS       sequence-34873-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Sun Z, Diaz Z, Fang X, Hart MP, Chesi A, Shorter J, Gitler AD
  TITLE     Molecular determinants and genetic modifiers of aggregation and 
            toxicity for the ALS disease protein FUS/TLS.
  JOURNAL   PLoS Biol. 2011 Apr;9(4):e1000614. doi: 
            10.1371/journal.pbio.1000614. Epub 2011 Apr 26.
  PUBMED    21541367
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2011-04"; volume: "9(4):e1000614. doi"; pages: " 
            10.1371/journal.pbio.1000614. Epub 2011 Apr 26"
COMMENT     Sequence Label: 426Gal-FUS
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..18
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2..18
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    26..50
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             436..447
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 ttctagaact agtggatccc ccatcacaag tttgtacaaa aaagcaggct ccaccatggc
       61 ctcaaacgat tatacccaac aagcaaccca aagctatggg gcctacccca cccagcccgg
      121 gcagggctat tcccagcaga gcagtcagcc ctacggacag cagagttaca gtggttatag
      181 ccagtccacg gacacttcag gctatggcca gagcagctat tcttcttatg gccagagcca
      241 gaacacaggc tatggaactc agtcaactcc ccagggatat ggctcgactg gcggctatgg
      301 cagtagccag agctcccaat cgtcttacgg gcagcagtcc tcctatcctg gctatggcca
      361 gcagccagct cccagcagca cctcgggaag ttacggtagc agttctcaga gcagcagcta
      421 tgggcagccc cagagtggga gctacagcca gcagcctagc tatggtggac agcagcaaag
      481 ctatggacag cagcaaagct ataatccccc tcagggctat ggacagcaga accagtacaa
      541 cagcagcagt ggtggtggag gtggaggtgg aggtggaggt aactatggcc aagatcaatc
      601 ctccatgagt agtggtggtg gcagtggtgg cggttatggc aatcaagacc agagtggtgg
      661 aggtggcagc ggtggctatg gacagcagga ccgtggaggc cgcggcaggg gtggcagtgg
      721 tggcggcggc ggcggcggcg gtggtggtta caaccgcagc agtggtggct atgaacccag
      781 aggtcgtgga ggtggccgtg gaggcagagg tggcatgggc ggaagtgacc gtggtggctt
      841 caataaa
//
