LOCUS       sequence-348757-        1758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependent expression of mitochondria-targeted GCaMP6f under 
            control of the EF1a promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Kole K, Voesenek BJB, Brinia ME, Petersen N, Kole MHP
  TITLE     Parvalbumin basket cell myelination accumulates axonal mitochondria 
            to internodes.
  JOURNAL   Nat Commun. 2022 Dec 9;13(1):7598. doi: 10.1038/s41467-022-35350-x.
  PUBMED    36494349
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-9"; volume: "13(1):7598. doi"; pages: " 
            10.1038/s41467-022-35350-x"
COMMENT     Sequence Label: pAAV-EF1a-4mtGCaMP6f-DIO
FEATURES             Location/Qualifiers
     source          1..1758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(1..441,442..447,448..882,883..900,901..1170,
                     1171..1176,1177..1233,1234..1260,1261..1284,1285..1287,
                     1288..1320,1321..1323,1324..1341,1342..1347))
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6f
                     /translation="GSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNKT
                     GHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGPV
                     LLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEEL
                     FTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTY
                     GVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIEL
                     KGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEEFSLFDKDGDGTITTKELGTVMRSL
                     GQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGNG
                     YISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     primer_bind     557..576
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     816..837
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(943..964)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      1245..1254
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(1378..1449)
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="SVLTPLLLRGLTGSARRLPVPRAK"
     CDS             complement(1480..1551)
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="SVLTPLLLRGLTGSARRLPVPRAK"
     CDS             complement(1582..1653)
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="SVLTPLLLRGLTGSARRLPVPRAK"
     CDS             complement(1684..1758)
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
ORIGIN
        1 cttcgctgtc atcatttgta caaactcttc gtagtttacc tgaccatccc catcgatgtc
       61 tgcttccctg atcatttcat caacctcttc atctgttaac ttctctccaa ggtttgtcat
      121 cacgtggcga agctctgctg cactgatgta gccattgcca tccttatcaa acacaccgaa
      181 cgcttctcta atttcttctt ccgtgtccct gtatttcatt tttcttgcca tcattgtcag
      241 gaactcaggg aagtcgattg tgccgtcacc gtcggcatct acttcattga tcatgtcctg
      301 cagctctgct tctgtggggt tctgccccag agaccgcatc accgtcccca gctccttggt
      361 tgttattgtc ccatccccgt ccttgtcaaa tagggagaat tcctctttaa attctgcgat
      421 ctgctcttca gtcagttggt ccggcaggtt gtactccagc ttgtgcccca ggatgttgcc
      481 gtcctccttg aagtcgatgc ccttcagctc gatgcggttc accagggtgt cgccctcgaa
      541 cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg
      601 gatgtagcct tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta
      661 gcggctgaag cactgcacgc cgtaggtcag ggtggtcacg agggtgggcc agggcacggg
      721 cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc
      781 accctcgccg gacacgctga acttgtggcc gtttacgtcg ccgtccagct cgaccaggat
      841 gggcaccacc ccggtgaaca gctcctcgcc cttgctcacc atgctccctc cggtaccgcc
      901 cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac
      961 catgtgatcg cgcttctcgt tggggtcttt cgaaagtttg gactgcacgc tcaggtagtg
     1021 gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtaggc
     1081 gagctgcacg ccgccgtcct cgatgttgtg gcggatcttg aagttcgcct tgatgccgtt
     1141 cttctgcttg tcggccttga tatagacgtt ctcgagtgag ctcagccgac ctatagctct
     1201 gactgcgtga cctgtcttat tccacttacg acgtgatgag tcgaccatgg tggcgagatc
     1261 cttatcgtca tcgtcgtaca gatcccgacc catttgctgt ccaccagtca tgctagccat
     1321 accatgatga tgatgatgat gagaaccaag cttcccctcc ggcggcaacg aatggatctt
     1381 ggcgcgcggc actgggagcc gccgggccga gcctgtcaag ccccgcagca gcagcggcgt
     1441 caggacggac agtttcccct ccggcggcaa cgaatggatc ttggcgcgcg gcactgggag
     1501 ccgccgggcc gagcctgtca agccccgcag cagcagcggc gtcaggacgg acagtttccc
     1561 ctccggcggc aacgaatgga tcttggcgcg cggcactggg agccgccggg ccgagcctgt
     1621 caagccccgc agcagcagcg gcgtcaggac ggacagtttc ccctccggcg gcaacgaatg
     1681 gatcttggcg cgcggcactg ggagccgccg ggccgagcct gtcaagcccc gcagcagcag
     1741 cggcgtcagg acggacat
//
