LOCUS       sequence-348791-        6321 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plgc-55B::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans AVB 
            premotor IN and other neurals expression of miniSOG-SL2 RFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6321)
  AUTHORS   Gao S, Guan SA, Fouad AD, Meng J, Kawano T, Huang YC, Li Y, Alcaire 
            S, Hung W, Lu Y, Qi YB, Jin Y, Alkema M, Fang-Yen C, Zhen M
  TITLE     Excitatory motor neurons are local oscillators for backward 
            locomotion.
  JOURNAL   Elife. 2018 Jan 23;7. pii: 29915. doi: 10.7554/eLife.29915.
  PUBMED    29360035
REFERENCE   2  (bases 1 to 6321)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-01-23"; volume: "7. pii: 29915. doi"; pages: " 
            10.7554/eLife.29915"
COMMENT     Sequence Label: pJH2890
FEATURES             Location/Qualifiers
     source          1..6321
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1217..1537
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
     primer_bind     complement(3761..3783)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3883..3902
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3980..3998)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4066..4170
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4171..4239,4240..5031)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4389..4408)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5202..5790
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5691..5710
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5944..5961
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6078..6099
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6114..6119,6120..6137,6138..6144)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6152..6168
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6157..6179
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6176..6192
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6176..6192
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgggc tgcagcatgc ggccgctgtt gcttttcttc tcctcacccc ccgtcgtctc
       61 ttctttttta ctattcccca cgagaaactg cccaccacaa aaacttactt tccagaaaaa
      121 tattggcaaa tagatgtcga aatgaaatgg gctagctttg atgatggtaa gcgttttttt
      181 tttctacaaa attgtggatt aagaatcttc caaaaccgtt atactacact atatccaaat
      241 ataaaaaaat tttggcccag gcggggggtc gaaccagggc cttcagattg gtagaccacc
      301 ccgttaccgc ctgagccact ttttcccatt tttgaagttg atggctaaga ttggagtttg
      361 aacgattgaa cgattttaga gcaaaatttt atggggattc tgaaaatata gtttatttta
      421 agtttggagc attttttatg ggctcaaaat gactcggaag ctcgaaaatc tgaaattttc
      481 aaaactttta atgtgggctt ttcaaatttc aaatttccgt gaaaaatatt gaatattggt
      541 tgtaaatttt aagaaatata tgaatttcaa cactttatta tacgaaaaac tgcatgcaaa
      601 aattttcagg tcaaatcatg tgcaaatttt gtgctgattt cgaattttta tatcattttg
      661 taccaaaacc cctagaattt tagaaacttt tgaacttctg ttaagtaatg actcactttt
      721 cgagcaaaat cgaatgttcc aaaaataggc aaaacatctg cccgcgcatt aagctcaacc
      781 acgacaattt tattggacag gaagttgttt ggtgactaat tgaatttcaa ttccccgtcc
      841 cccctctcca cacaaatacc actacgaaca tctcttttga cggatccccg ggattggcca
      901 aaggacccaa aggtatgttt cgaatgatac taacataaca tagaacattt tcaggaggac
      961 ccttggctag cgtcgacgga tcccgatgtc ggacacaatt cttggtttca acaaatcaaa
     1021 cgtcgttttg gctgctggaa ttgctggagc cgctttcctc ggctactgca tttacttcga
     1081 tcataagaga atcaacgctc cagactacaa ggacaagatt aggcaaagtc agtgttttaa
     1141 caacatattt ccttcggatt tttatctaaa aacaacttat tttctttcag agagacgtgc
     1201 ccaggctggg gatcctatgg agaaaagttt cgtgataact gatccacggc tgccagacaa
     1261 tcccatcatc ttcgcatccg atggcttcct ggagctgacc gagtattcca gagaggagat
     1321 cctgggccgc aatggccgct ttctgcaggg accagagaca gaccaggcca cagtgcagaa
     1381 gattcgcgat gccattagag atcagcgcga gattaccgtg cagctgataa actacacaaa
     1441 aagcgggaag aaattctgga acctcctgca cctccagccc atgagggacc agaagggtga
     1501 gctccagtat ttcatcggag tgcagctgga tggataaaga tccccgagat cttcagcgct
     1561 cagcattcgt agaattgtaa gtttaaactt tctcatacta actaacgcgc tctatttaaa
     1621 ttttcagaat tcgctgtctc atcctacttt cacctagtta actgcttgtc ttaaaatcta
     1681 tgcttctctt tagtatctaa aattttccta gaagcttaca agtatataaa tggtctcttc
     1741 tcaataaagg ttgtatattt attcatctta ttgaatctgc catttcctcg tttttgcgag
     1801 tttatatacc ttccaatttt ctttctattg tattttcaac ttctaatttt aattcaggga
     1861 aactgcttca acgcatcggt accctatggt ctcaaagggt gaagaagata acatggcaat
     1921 tattaaagag tttatgcgtt tcaaggtgca tatggaggga tctgtcaatg ggcatgagtt
     1981 tgaaattgaa ggtgaaggag aaggccgacc atatgaggga acacaaaccg caaaactaaa
     2041 ggtaagttta aacatatata tactaactaa ccctgattat ttaaattttc aggtaactaa
     2101 aggcggacca ttaccattcg cctgggacat cctctctcca cagttcatgt atggaagtaa
     2161 agcttatgtt aaacatccgg cagatatacc agattatttg aaactttcat tcccggaggg
     2221 ttttaagtgg gaacgcgtaa tgaattttga agacggagga gttgttacag tgacgcaaga
     2281 ctcaaggtaa gtttaaacag ttcggtacta actaaccata catatttaaa ttttcagcct
     2341 ccaagatgga gaatttattt ataaagtcaa acttcgagga acgaatttcc cctcggatgg
     2401 atctgttatg cagaagaaga ctatgggatg ggaagcttca agtgaaagaa tgtaccctga
     2461 agacggtgct cttaagggag agattaaaca acgtcttaaa ttgaaagatg gaggacatta
     2521 cgatgctgag gtaagtttaa acatgatttt actaactaac taatctgatt taaattttca
     2581 ggtgaagaca acttacaaag ccaaaaaacc agttcagctg ccaggagcgt acaatgttaa
     2641 tattaaactg gatatcacct cccacaacga ggattacact atcgttgagc aatatgaaag
     2701 agctgaaggg cggcactcga caggtggcat ggatgaattg tataagtagg agctcccgag
     2761 atcttcagcg ctcagcattc gtagaattgt aagtttaaac tttctcatac taactaacgc
     2821 gctctattta aattttcaga attccaactg agcgccggtc gctaccatta ccaacttgtc
     2881 tggtgtcaaa aataataggg gccgctgtca tcagagtaag tttaaactga gttctactaa
     2941 ctaacgagta atatttaaat tttcagcatc tcgcgcccgt gcctctgact tctaagtcca
     3001 attactcttc aacatcccta catgctcttt ctccctgtgc tcccaccccc tatttttgtt
     3061 attatcaaaa aaacttcttc ttaatttctt tgttttttag cttcttttaa gtcacctcta
     3121 acaatgaaat tgtgtagatt caaaaataga attaattcgt aataaaaagt cgaaaaaaat
     3181 tgtgctccct ccccccatta ataataattc tatcccaaaa tctacacaat gttctgtgta
     3241 cacttcttat gtttttttta cttctgataa attttttttg aaacatcata gaaaaaaccg
     3301 cacacaaaat accttatcat atgttacgtt tcagtttatg accgcaattt ttatttcttc
     3361 gcacgtctgg gcctctcatg acgtcaaatc atgctcatcg tgaaaaagtt ttggagtatt
     3421 tttggaattt ttcaatcaag tgaaagttta tgaaattaat tttcctgctt ttgctttttg
     3481 ggggtttccc ctattgtttg tcaagagttt cgaggacggc gtttttcttg ctaaaatcac
     3541 aagtattgat gagcacgatg caagaaagat cggaagaagg tttgggtttg aggctcagtg
     3601 gaaggtgagt agaagttgat aatttgaaag tggagtagtg tctatggggt ttttgcctta
     3661 aatgacagaa tacattccca atataccaaa cataactgtt tcctactagt cggccgtacg
     3721 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     3781 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     3841 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
     3901 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     3961 gcatcaggcg gccttaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     4021 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     4081 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     4141 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     4201 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     4261 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     4321 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     4381 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     4441 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     4501 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     4561 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     4621 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     4681 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     4741 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     4801 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     4861 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     4921 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     4981 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     5041 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     5101 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     5161 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     5221 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     5281 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     5341 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     5401 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     5461 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     5521 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     5581 ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     5641 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     5701 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     5761 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     5821 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     5881 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     5941 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     6001 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     6061 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     6121 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     6181 aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta ctaactaact
     6241 attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac gatggatacg
     6301 ctaacaactt ggaaatgaaa t
//
