LOCUS       sequence-348848-       10625 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector plasmid for expressing spCas9-Klenow fusion protein and 
            sgRNA. There are two copies of HBB 3' UTR in the 3'UTR of 
            spCas9-Klenow to enhance expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10625)
  AUTHORS   Yoo KW, Yadav MK, Song Q, Atala A, Lu B
  TITLE     Targeting DNA polymerase to DNA double-strand breaks reduces DNA 
            deletion size and increases templated insertions generated by 
            CRISPR/Cas9.
  JOURNAL   Nucleic Acids Res. 2022 Mar 22. pii: 6552087. doi: 
            10.1093/nar/gkac186.
  PUBMED    35323942
REFERENCE   2  (bases 1 to 10625)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2022 Mar 22. pii: 6552087. doi: 10.1093/nar/gkac186."
COMMENT     Sequence Label: pspCas9-Klenow-ST2-com-vector
FEATURES             Location/Qualifiers
     source          1..10625
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        6..291
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     intron          571..798
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      810..819
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             819..884
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             891..911
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             936..5036
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             936..5036
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5037..5084
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(7284..7301)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7290..7497
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   7509..7649
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   7509..7638
                     /label=AAV2 ITR
     rep_origin      7724..8179
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7811..7830)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8021..8042
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8196..8215)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8315..8337
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8375..8393)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8461..8565
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8566..8634,8635..9426)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8784..8803)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9597..10185
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10086..10105
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        10247..10495
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     10247..10267
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     10418..10437
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 gtacccgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
       61 ccattgacgt caatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
      121 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
      181 attgacgtca atgacggtaa atggcccgcc tggcattgtg cccagtacat gaccttatgg
      241 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtcgaggtg
      301 agccccacgt tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat
      361 ttatttattt tttaattatt ttgtgcagcg atgggggcgg gggggggggg ggggcgcgcg
      421 ccrggsgggg sggggsgggg sgrggggsgg ggsggggsgr ggsggrgrgg kgsggcggca
      481 gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg
      541 ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg cgcgctgcct tcgccccgtg
      601 ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca
      661 caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagctgag caagaggtaa
      721 gggtttaagg gatggttggt tggtggggta ttaatgttta attacctgga gcacctgcct
      781 gaaatcactt tttttcaggt tggaccggtg ccaccatgga ctataaggac cacgacggag
      841 actacaagga tcatgatatt gattacaaag acgatgacga taagatggcc ccaaagaaga
      901 agcggaaggt cggtatccac ggagtcccag cagccgacaa gaagtacagc atcggcctgg
      961 acatcggcac caactctgtg ggctgggccg tgatcaccga cgagtacaag gtgcccagca
     1021 agaaattcaa ggtgctgggc aacaccgacc ggcacagcat caagaagaac ctgatcggag
     1081 ccctgctgtt cgacagcggc gaaacagccg aggccacccg gctgaagaga accgccagaa
     1141 gaagatacac cagacggaag aaccggatct gctatctgca agagatcttc agcaacgaga
     1201 tggccaaggt ggacgacagc ttcttccaca gactggaaga gtccttcctg gtggaagagg
     1261 ataagaagca cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg gcctaccacg
     1321 agaagtaccc caccatctac cacctgagaa agaaactggt ggacagcacc gacaaggccg
     1381 acctgcggct gatctatctg gccctggccc acatgatcaa gttccggggc cacttcctga
     1441 tcgagggcga cctgaacccc gacaacagcg acgtggacaa gctgttcatc cagctggtgc
     1501 agacctacaa ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg gacgccaagg
     1561 ccatcctgtc tgccagactg agcaagagca gacggctgga aaatctgatc gcccagctgc
     1621 ccggcgagaa gaagaatggc ctgttcggaa acctgattgc cctgagcctg ggcctgaccc
     1681 ccaacttcaa gagcaacttc gacctggccg aggatgccaa actgcagctg agcaaggaca
     1741 cctacgacga cgacctggac aacctgctgg cccagatcgg cgaccagtac gccgacctgt
     1801 ttctggccgc caagaacctg tccgacgcca tcctgctgag cgacatcctg agagtgaaca
     1861 ccgagatcac caaggccccc ctgagcgcct ctatgatcaa gagatacgac gagcaccacc
     1921 aggacctgac cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag tacaaagaga
     1981 ttttcttcga ccagagcaag aacggctacg ccggctacat tgacggcgga gccagccagg
     2041 aagagttcta caagttcatc aagcccatcc tggaaaagat ggacggcacc gaggaactgc
     2101 tcgtgaagct gaacagagag gacctgctgc ggaagcagcg gaccttcgac aacggcagca
     2161 tcccccacca gatccacctg ggagagctgc acgccattct gcggcggcag gaagattttt
     2221 acccattcct gaaggacaac cgggaaaaga tcgagaagat cctgaccttc cgcatcccct
     2281 actacgtggg ccctctggcc aggggaaaca gcagattcgc ctggatgacc agaaagagcg
     2341 aggaaaccat caccccctgg aacttcgagg aagtggtgga caagggcgct tccgcccaga
     2401 gcttcatcga gcggatgacc aacttcgata agaacctgcc caacgagaag gtgctgccca
     2461 agcacagcct gctgtacgag tacttcaccg tgtataacga gctgaccaaa gtgaaatacg
     2521 tgaccgaggg aatgagaaag cccgccttcc tgagcggcga gcagaaaaag gccatcgtgg
     2581 acctgctgtt caagaccaac cggaaagtga ccgtgaagca gctgaaagag gactacttca
     2641 agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg ttcaacgcct
     2701 ccctgggcac ataccacgat ctgctgaaaa ttatcaagga caaggacttc ctggacaatg
     2761 aggaaaacga ggacattctg gaagatatcg tgctgaccct gacactgttt gaggacagag
     2821 agatgatcga ggaacggctg aaaacctatg cccacctgtt cgacgacaaa gtgatgaagc
     2881 agctgaagcg gcggagatac accggctggg gcaggctgag ccggaagctg atcaacggca
     2941 tccgggacaa gcagtccggc aagacaatcc tggatttcct gaagtccgac ggcttcgcca
     3001 acagaaactt catgcagctg atccacgacg acagcctgac ctttaaagag gacatccaga
     3061 aagcccaggt gtccggccag ggcgatagcc tgcacgagca cattgccaat ctggccggca
     3121 gccccgccat taagaagggc atcctgcaga cagtgaaggt ggtggacgag ctcgtgaaag
     3181 tgatgggccg gcacaagccc gagaacatcg tgatcgaaat ggccagagag aaccagacca
     3241 cccagaaggg acagaagaac agccgcgaga gaatgaagcg gatcgaagag ggcatcaaag
     3301 agctgggcag ccagatcctg aaagaacacc ccgtggaaaa cacccagctg cagaacgaga
     3361 agctgtacct gtactacctg cagaatgggc gggatatgta cgtggaccag gaactggaca
     3421 tcaaccggct gtccgactac gatgtggacc atatcgtgcc tcagagcttt ctgaaggacg
     3481 actccatcga caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag agcgacaacg
     3541 tgccctccga agaggtcgtg aagaagatga agaactactg gcggcagctg ctgaacgcca
     3601 agctgattac ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc ggcctgagcg
     3661 aactggataa ggccggcttc atcaagagac agctggtgga aacccggcag atcacaaagc
     3721 acgtggcaca gatcctggac tcccggatga acactaagta cgacgagaat gacaagctga
     3781 tccgggaagt gaaagtgatc accctgaagt ccaagctggt gtccgatttc cggaaggatt
     3841 tccagtttta caaagtgcgc gagatcaaca actaccacca cgcccacgac gcctacctga
     3901 acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc gagttcgtgt
     3961 acggcgacta caaggtgtac gacgtgcgga agatgatcgc caagagcgag caggaaatcg
     4021 gcaaggctac cgccaagtac ttcttctaca gcaacatcat gaactttttc aagaccgaga
     4081 ttaccctggc caacggcgag atccggaagc ggcctctgat cgagacaaac ggcgaaaccg
     4141 gggagatcgt gtgggataag ggccgggatt ttgccaccgt gcggaaagtg ctgagcatgc
     4201 cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc aaagagtcta
     4261 tcctgcccaa gaggaacagc gataagctga tcgccagaaa gaaggactgg gaccctaaga
     4321 agtacggcgg cttcgacagc cccaccgtgg cctattctgt gctggtggtg gccaaagtgg
     4381 aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct gctggggatc accatcatgg
     4441 aaagaagcag cttcgagaag aatcccatcg actttctgga agccaagggc tacaaagaag
     4501 tgaaaaagga cctgatcatc aagctgccta agtactccct gttcgagctg gaaaacggcc
     4561 ggaagagaat gctggcctct gccggcgaac tgcagaaggg aaacgaactg gccctgccct
     4621 ccaaatatgt gaacttcctg tacctggcca gccactatga gaagctgaag ggctcccccg
     4681 aggataatga gcagaaacag ctgtttgtgg aacagcacaa gcactacctg gacgagatca
     4741 tcgagcagat cagcgagttc tccaagagag tgatcctggc cgacgctaat ctggacaaag
     4801 tgctgtccgc ctacaacaag caccgggata agcccatcag agagcaggcc gagaatatca
     4861 tccacctgtt taccctgacc aatctgggag cccctgccgc cttcaagtac tttgacacca
     4921 ccatcgaccg gaagaggtac accagcacca aagaggtgct ggacgccacc ctgatccacc
     4981 agagcatcac cggcctgtac gagacacgga tcgacctgtc tcagctggga ggcgacaaaa
     5041 ggccggcggc cacgaaaaag gccggccagg caaaaaagaa aaagggatcc tctgagacac
     5101 caggcacctc cgagtctgcc acccctgagg gaggagcacc agaggtgaca gccaccgtga
     5161 tctcctacga taactatgtg acaatcctgg acgaggagac actgaaggcc tggatcgcca
     5221 agctggagaa ggcccccgtg ttcgcctttg atacagagac agacagcctg gataacatct
     5281 ccgccaatct ggtgggcctg tctttcgcca tcgagcctgg cgtggccgcc tatatcccag
     5341 tggcccacga ctacctggat gcccccgacc agatcagcag ggagagagcc ctggagctgc
     5401 tgaagcctct gctggaggat gagaaggccc tgaaggtcgg ccagaacctg aagtatgaca
     5461 ggggcatcct ggccaattac ggcatcgagc tgagaggcat cgcctttgac accatgctgg
     5521 agtcttatat cctgaatagc gtggcaggcc ggcacgacat ggattccctg gccgagaggt
     5581 ggctgaagca caagacaatc accttcgagg agatcgccgg caagggcaag aaccagctga
     5641 ccttcaacca gatcgccctg gaggaggcag gcaggtacgc agcagaggac gcagatgtga
     5701 ccctgcagct gcacctgaag atgtggccag atctgcagaa gcacaagggc cccctgaacg
     5761 tctttgagaa tatcgagatg cccctggtgc ctgtgctgag ccggatcgag cgcaacggcg
     5821 tgaagatcga ccctaaggtg ctgcacaatc actccgagga gctgaccctg agactggccg
     5881 agctggagaa gaaggcccac gagatcgccg gcgaggagtt caacctgtcc tctacaaagc
     5941 agctgcagac catcctgttt gagaagcagg gcatcaagcc cctgaagaaa acccctggag
     6001 gagcaccatc taccagcgag gaggtgctgg aggagctggc cctggattat cccctgccta
     6061 aagtgatcct ggagtaccgg ggcctggcca agctgaagtc tacatatacc gacaagctgc
     6121 ccctgatgat caaccctaag acaggccggg tgcacaccag ctaccaccag gcagtgacag
     6181 caaccggccg cctgagctcc accgatccaa acctgcagaa tatccccgtg aggaatgagg
     6241 agggcaggag aatcagacag gccttcatcg cccccgagga ttatgtgatc gtgtccgccg
     6301 actactctca gatcgagctg aggatcatgg cccacctgtc cagagataag ggcctgctga
     6361 cagccttcgc cgagggcaag gacatccaca gggcaaccgc agcagaggtg tttggcctgc
     6421 ctctggagac agtgacctcc gagcagcggc gctctgccaa ggccatcaac ttcggcctga
     6481 tctatggcat gtctgccttt ggcctggcca ggcagctgaa tatccctaga aaggaggccc
     6541 agaagtacat ggacctgtat ttcgagagat accccggcgt gctggagtac atggagagga
     6601 cacgcgcaca ggcaaaggag cagggctatg tggagacact ggatggcagg agactgtacc
     6661 tgccagacat caagtctagc aacggagcaa ggagggcagc agcagagagg gccgccatca
     6721 atgcccccat gcagggcaca gccgccgata tcatcaagag agccatgatc gcagtggacg
     6781 cctggctgca ggcagagcag ccaagggtga gaatgatcat gcaggtgcac gatgagctgg
     6841 tgtttgaggt gcacaaggac gatgtggacg ccgtggccaa gcagatccac cagctgatgg
     6901 agaactgtac ccgcctggat gtgccactgc tggtggaagt gggaagcgga gagaattggg
     6961 accaggccca ctgaggatcc taagctcgct ttcttgctgt ccaatttcta ttaaaggttc
     7021 ctttgttccc taagtccaac tactaaactg ggggatatta tgaagggcct tgagcatctg
     7081 gattctgcct aataaaaaac atttattttc attgctagct cgctttcttg ctgtccaatt
     7141 tctattaaag gttcctttgt tccctaagtc caactactaa actgggggat attatgaagg
     7201 gccttgagca tctggattct gcctaataaa aaacatttat tttcattgcg aattctagaa
     7261 aattcctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     7321 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     7381 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     7441 ggtggggcag gacagcaagg gggaggattg ggaagagaat agcaggcatg ctggggaggt
     7501 acggccgcag gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct
     7561 cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt
     7621 gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc tccttacgca
     7681 tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc
     7741 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     7801 ttagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     7861 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     7921 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     7981 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     8041 ggaacaacac tcaactctat ctcgggctat tcttttgatt tataagggat tttgccgatt
     8101 tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     8161 atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag
     8221 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     8281 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     8341 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     8401 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     8461 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     8521 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     8581 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     8641 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     8701 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     8761 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     8821 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     8881 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     8941 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     9001 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     9061 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     9121 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     9181 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     9241 ggctggttta ttgctgataa atctggagcc ggtgagcgtg gaagccgcgg tatcattgca
     9301 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     9361 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     9421 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     9481 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     9541 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     9601 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     9661 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     9721 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     9781 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     9841 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     9901 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     9961 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
    10021 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
    10081 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
    10141 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
    10201 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgtgagg gcctatttcc
    10261 catgattcct tcatatttgc atatacgata caaggctgtt agagagataa ttggaattaa
    10321 tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct
    10381 tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac
    10441 ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccggtgt
    10501 cttcctcgag gaagacccgt ttgagagcta gaaatagcaa gttcaaataa ggctagtccg
    10561 ttatcaactt ggctgaatgc ctgcgagcat cccacccaag tggcaccgag tcggtgcttt
    10621 ttttg
//
