LOCUS       sequence-348855-        9128 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CAG-mTagBFP2-T2A-iCre-ERT2 piggyBac transposon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9128)
  AUTHORS   Nam HS, Capecchi MR
  TITLE     piggyBac transposon plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9128)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PB CAG-mTagBFP2-T2A-iCreERT2
FEATURES             Location/Qualifiers
     source          1..9128
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        66..170
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(171..239,240..1031)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(389..408)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1202..1790
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1691..1710
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1944..1961
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2078..2099
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2114..2119,2120..2137,2138..2144)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2152..2168
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2157..2179
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2176..2192
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2176..2192
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2211..2231
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2213..2231
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   2295..2357
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        2662..3041
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3043..3296
                     /label=chicken beta-actin promoter
                     /note="contains a 21-bp deletion relative to the standard 
                     chicken beta-actin promoter"
     intron          3297..4313
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4237..4259
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4321..4340
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      4376..4385
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4382..5092
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             5114..5167
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6218..7153
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             6266..7159
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     misc_feature    7196..7784
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7249..7269)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7667..7678)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7823..7840)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7829..8053
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   8407..8441
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8528..8547)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8529..8547)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8554..8571)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8554..8570)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8563..8585)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(join(8712..9128,1..39))
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8849..8870)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9061..9080
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa
       61 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
      121 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
      181 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
      241 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
      301 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
      361 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
      421 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
      481 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
      541 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
      601 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
      661 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
      721 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
      781 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
      841 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
      901 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
      961 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     1021 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     1081 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     1141 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     1201 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     1261 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     1321 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     1381 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     1441 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     1501 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     1561 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     1621 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     1681 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     1741 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     1801 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     1861 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     1921 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     1981 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     2041 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     2101 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     2161 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
     2221 ctcactaaag ggaacctccc ccggtattca cgacagcagg ctgaataata aaaaaattag
     2281 aaactattat ttaaccctag aaagataatc atattgtgac gtacgttaaa gataatcatg
     2341 cgtaaaattg acgcatgtgt tttatcggtc tgtatatcga ggtttattta ttaatttgaa
     2401 tagatattaa gttttattat atttacactt acatactaat aataaattca acaaacaatt
     2461 tatttatgtt tatttattta ttaaaaaaaa acaaaaactc aaaatttctt ctataaagta
     2521 acaaaacttt taaacattct ctcttttaca aaaataaact tattttgtac tttaaaaaca
     2581 gtcatgttgt attataaaat aagtaattag cttaacctat acataataga aacaaattat
     2641 acttattaac cggtcatggt cgacattgat tattgactag ttattaatag taatcaatta
     2701 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     2761 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     2821 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     2881 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
     2941 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
     3001 cttggcagta catctacgta ttagtcatcg ctattaccat ggtcgaggtg agccccacgt
     3061 tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt
     3121 tttaattatt ttgtgcagcg atgggggcgg gggggggggg ggggcgcgcg gggcggggcg
     3181 gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt
     3241 ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag
     3301 tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc
     3361 ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg
     3421 gctgtaatta gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc
     3481 ttgaggggct ccgggagggc cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt
     3541 gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg
     3601 ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg
     3661 gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     3721 tgggggggtg agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc
     3781 ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg
     3841 cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc
     3901 cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg cccccggagc gccggcggct
     3961 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     4021 gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct
     4081 ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc
     4141 ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca gcctcggggc tgtccgcggg
     4201 gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg
     4261 gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg
     4321 gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattgcta gcgccgccac
     4381 catggtgtct aagggcgaag agctgattaa ggagaacatg cacatgaagc tgtacatgga
     4441 gggcaccgtg gacaaccatc acttcaagtg cacatccgag ggcgaaggca agccctacga
     4501 gggcacccag accatgagaa tcaaggtggt cgagggcggc cctctcccct tcgccttcga
     4561 catcctggct actagcttcc tctacggcag caagaccttc atcaaccaca cccagggcat
     4621 ccccgacttc ttcaagcagt ccttccctga gggcttcaca tgggagagag tcaccacata
     4681 cgaagacggg ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat
     4741 ctacaacgtc aagatcagag gggtgaactt cacatccaac ggccctgtga tgcagaagaa
     4801 aacactcggc tgggaggcct tcaccgagac gctgtacccc gctgacggcg gcctggaagg
     4861 cagaaacgac atggccctga agctcgtggg cgggagccat ctgatcgcaa acgccaagac
     4921 cacatataga tccaagaaac ccgctaagaa cctcaagatg cctggcgtct actatgtgga
     4981 ctacagactg gaaagaatca aggaggccaa caacgagacc tacgtcgagc agcacgaggt
     5041 ggcagtggcc agatactgcg acctccctag caaactgggg cacaagctta atggaggatc
     5101 tggcggaaga tctgaaggcc gcggcagcct gctgacatgc ggcgacgtgg aagagaaccc
     5161 tggccctgcc ggcagcgcca gcaacctgct gactgtgcac caaaacctgc ctgccctccc
     5221 tgtggatgcc acctctgatg aagtcaggaa gaacctgatg gacatgttca gggacaggca
     5281 ggccttctct gaacacacct ggaagatgct cctgtctgtg tgcagatcct gggctgcctg
     5341 gtgcaagctg aacaacagga aatggttccc tgctgaacct gaggatgtga gggactacct
     5401 cctgtacctg caagccagag gcctggctgt gaagaccatc caacagcacc tgggccagct
     5461 caacatgctg cacaggagat ctggcctgcc tcgcccttct gactccaatg ctgtgtccct
     5521 ggtgatgagg agaatcagaa aggagaatgt ggatgctggg gagagagcca agcaggccct
     5581 ggcctttgaa cgcactgact ttgaccaagt cagatccctg atggagaact ctgacagatg
     5641 ccaggacatc aggaacctgg ccttcctggg cattgcctac aacaccctgc tgcgcattgc
     5701 cgaaattgcc agaatcagag tgaaggacat ctcccgcacc gatggtggga gaatgctgat
     5761 ccacattggc aggaccaaga ccctggtgtc cacagctggt gtggagaagg ccctgtccct
     5821 gggggttacc aagctggtgg agagatggat ctctgtgtct ggtgtggctg atgaccccaa
     5881 caactacctg ttctgccggg tcagaaagaa tggtgtggct gccccttctg ccacctccca
     5941 actgtccacc cgggccctgg aagggatctt tgaggccacc caccgcctga tctatggtgc
     6001 caaggatgac tctgggcaga gatacctggc ctggtctggc cactctgcca gagtgggtgc
     6061 tgccagggac atggccaggg ctggtgtgtc catccctgaa atcatgcagg ctggtggctg
     6121 gaccaatgtg aacattgtga tgaactacat cagaaacctg gactctgaga ctggggccat
     6181 ggtgaggctg ctcgaggatg gggacctcga gccatctgct ggagacatga gagctgccaa
     6241 cctttggcca agcccgctca tgatcaaacg ctctaagaag aacagcctgg ccttgtccct
     6301 gacggccgac cagatggtca gtgccttgtt ggatgctgag ccccccatac tctattccga
     6361 gtatgatcct accagaccct tcagtgaagc ttcgatgatg ggcttactga ccaacctggc
     6421 agacagggag ctggttcaca tgatcaactg ggcgaagagg gtgccaggct ttgtggattt
     6481 gaccctccat gatcaggtcc accttctaga atgtgcctgg ctagagatcc tgatgattgg
     6541 tctcgtctgg cgctccatgg agcacccagt gaagctactg tttgctccta acttgctctt
     6601 ggacaggaac cagggaaaat gtgtagaggg catggtggag atcttcgaca tgctgctggc
     6661 tacatcatct cggttccgca tgatgaatct gcagggagag gagtttgtgt gcctcaaatc
     6721 tattattttg cttaattctg gagtgtacac atttctgtcc agcaccctga agtctctgga
     6781 agagaaggac catatccacc gagtcctgga caagatcaca gacactttga tccacctgat
     6841 ggccaaggca ggcctgaccc tgcagcagca gcaccagcgg ctggcccagc tcctcctcat
     6901 cctctcccac atcaggcaca tgagtaacaa aggcatggag catctgtaca gcatgaagtg
     6961 caagaacgtg gtgcccctct atgacctgct gctggaggcg gcggacgccc accgcctaca
     7021 tgcgcccact agccgtggag gggcatccgt ggaggagacg gaccaaagcc acttggccac
     7081 tgcgggctct acttcatcgc attccttgca aaagtattac atcacggggg aggcagaggg
     7141 tttccctgcc acagcttgat taattaatga tcatacagtg ctctgagtac gctataatca
     7201 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     7261 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     7321 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     7381 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     7441 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     7501 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     7561 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     7621 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     7681 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     7741 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcaggatc cactagagcg
     7801 gccgcctaga gctcgctatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     7861 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     7921 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     7981 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     8041 gtgggctcta tggcttctga ggcggaaaga accagctggg gctcgactag agcttgcgga
     8101 acccttaagc ttttttatta tatattagtc acgatatcta taacaagaaa atatatatat
     8161 aataagttat cacgtaagta gaacatgaaa taacaatata attatcgtat gagttaaatc
     8221 ttaaaagtca cgtaaaagat aatcatgcgt cattttgact cacgcggtcg ttatagttca
     8281 aaatcagtga cacttaccgc attgacaagc acgcctcacg ggagctccaa gcggcgactg
     8341 agatgtccta aatgcacagc gacggattcg cgctatttag aaagagagag caatatttca
     8401 agaatgcatg cgtcaatttt acgcagacta tctttctagg gttaaaaaag atttgcgctt
     8461 tactcgacct aaactttaaa cacgtcatag aatcttcgtt tgacaaaaac cacattgtgt
     8521 tgggtcgccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga
     8581 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     8641 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     8701 tggcgaatgg caaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     8761 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     8821 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     8881 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     8941 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     9001 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     9061 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     9121 cgtaacca
//
