LOCUS       sequence-348921-        8566 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac plasmid expressing EGFP under the control of a cone 
            arrestin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8566)
  AUTHORS   Gasparini SJ, Tessmer K, Reh M, Wieneke S, Carido M, Volkner M, 
            Borsch O, Swiersy A, Zuzic M, Goureau O, Kurth T, Busskamp V, Zeck 
            G, Karl MO, Ader M
  TITLE     Transplanted human cones incorporate and function in a murine cone 
            degeneration model.
  JOURNAL   J Clin Invest. 2022 Apr 28. pii: 154619. doi: 10.1172/JCI154619.
  PUBMED    35482419
REFERENCE   2  (bases 1 to 8566)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2022 Apr 28. pii: 154619. doi: 10.1172/JCI154619."
COMMENT     Sequence Label: PB-mCar-EGFP-WPRE-BGH-pA-UBC-Bla
FEATURES             Location/Qualifiers
     source          1..8566
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(7..111)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(137..592)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(274..295)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     486..505
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     719..741
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     733..750
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     734..750
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   880..942
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     regulatory      1896..1905
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1902..1904,1905..1907,1908..2621)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1902..1904,1905..1907,1908..2618)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1947..1968)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2208..2227)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2555..2576
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2629..3217
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2682..2702)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3100..3111)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3224..3448
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        3460..4671
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4508..4527
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      4701..4710
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4707..5105
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYAWEG"
     polyA_signal    5144..5265
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5181..5200)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5235..5254
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   6026..6060
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6551..6567)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6551..6567)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6564..6586)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6575..6591
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6599..6605,6606..6623,6624..6629))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6644..6665
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6782..6799)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6953..7541)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7033..7052)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7712..8503,8504..8566,1..6))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8335..8354
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
       61 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
      121 ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
      181 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
      241 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
      301 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
      361 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
      421 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
      481 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
      541 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg
      601 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
      661 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
      721 cagtcacgac gttgtaaaac gacggccagt gagcgcgcct cgttcattca cgtttttgaa
      781 cccgtggagg acgggcagac tcgcggtgca aatgtgtttt acagcgtgat ggagcagatg
      841 aagatgctcg acacgctgca gaacacgcag ctagattaac cctagaaaga taatcatatt
      901 gtgacgtacg ttaaagataa tcatgtgtaa aattgacgca tgtgttttat cggtctgtat
      961 atcgaggttt atttattaat ttgaatagat attaagtttt attatattta cacttacata
     1021 ctaataataa attcaacaaa caatttattt atgtttattt atttattaaa aaaaacaaaa
     1081 actcaaaatt tcttctataa agtaacaaaa cttttatgag ggacagcccc cccccaaagc
     1141 ccccagggat gtaattacgt ccctcccccg ctagggggca gcagcgagcc gcccggggct
     1201 ccgctccggt ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg
     1261 gcacggggaa ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc
     1321 tgcagacacc tggggggata cggggaaaag gcctccacgg ccactagtgg ttcttcccat
     1381 tttggctaca tggtcttttt ttttaccttt ttggttcctt tggccttttg gcttttggct
     1441 tccagggctt ctggatcccc cccaacccct cccatacaca tacacatgtg cactcgtgca
     1501 ctcaacccag cacaggataa tgttcattct tgacctttcc acatacatct ggctatgttc
     1561 tctctcttat ctacaataaa tctcctccac tatacttagg agcagttatg ttcttcttct
     1621 ttctttcttt tttttttttt tcattcagta acatcatcag aatcccctag ctctggccta
     1681 cctcctcagt aacaatcagc tgatccctgg ccactaatct gtactcacta atctgttttc
     1741 caaactcttg gcccctgagc taattatagc agtgcttcat gccacccacc ccaaccctat
     1801 tcttgttctc tgactcccac taatctacac attcagagga ttgtggatat aagaggctgg
     1861 gaggccagct tagcaaccag agctggaggt ctagagccac catggtgagc aagggcgagg
     1921 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     1981 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     2041 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     2101 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     2161 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     2221 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     2281 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     2341 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     2401 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     2461 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     2521 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     2581 ccgccgggat cactctcggc atggacgagc tgtacaagtg aggatcctaa tcaacctctg
     2641 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     2701 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     2761 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     2821 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     2881 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     2941 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     3001 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     3061 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     3121 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     3181 cagacgagtc ggatctccct ttgggccgcc tccccgcgaa ttcctgtgcc ttctagttgc
     3241 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     3301 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     3361 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     3421 catgctgggg atgcggtggg ctctatggat cgatgatctg gcctccgcgc cgggttttgg
     3481 cgcctcccgc gggcgccccc ctcctcacgg cgagcgctgc cacgtcagac gaagggcgca
     3541 gcgagcgtcc tgatccttcc gcccggacgc tcaggacagc ggcccgctgc tcataagact
     3601 cggccttaga accccagtat cagcagaagg acattttagg acgggacttg ggtgactcta
     3661 gggcactggt tttctttcca gagagcggaa caggcgagga aaagtagtcc cttctcggcg
     3721 attctgcgga gggatctccg tggggcggtg aacgccgatg attatataag gacgcgccgg
     3781 gtgtggcaca gctagttccg tcgcagccgg gatttgggtc gcggttcttg tttgtggatc
     3841 gctgtgatcg tcacttggtg agtagcgggc tgctgggctg gccggggctt tcgtggccgc
     3901 cgggccgctc ggtgggacgg aagcgtgtgg agagaccgcc aagggctgta gtctgggtcc
     3961 gcgagcaagg ttgccctgaa ctgggggttg gggggagcgc agcaaaatgg cggctgttcc
     4021 cgagtcttga atggaagacg cttgtgaggc gggctgtgag gtcgttgaaa caaggtgggg
     4081 ggcatggtgg gcggcaagaa cccaaggtct tgaggccttc gctaatgcgg gaaagctctt
     4141 attcgggtga gatgggctgg ggcaccatct ggggaccctg acgtgaagtt tgtcactgac
     4201 tggagaactc ggtttgtcgt ctgttgcggg ggcggcagtt atggcggtgc cgttgggcag
     4261 tgcacccgta cctttgggag cgcgcgccct cgtcgtgtcg tgacgtcacc cgttctgttg
     4321 gcttataatg cagggtgggg ccacctgccg gtaggtgtgc ggtaggcttt tctccgtcgc
     4381 aggacgcagg gttcgggcct agggtaggct ctcctgaatc gacaggcgcc ggacctctgg
     4441 tgaggggagg gataagtgag gcgtcagttt ctttggtcgg ttttatgtac ctatcttctt
     4501 aagtagctga agctccggtt ttgaactatg cgctcggggt tggcgagtgt gttttgtgaa
     4561 gttttttagg caccttttga aatgtaatca tttgggtcaa tatgtaattt tcagtgttag
     4621 actagtaaat tgtccgctaa attctggccg tttttggctt ttttgttaga cgaagcttgg
     4681 gctgcaggtc gactctagat gccaccatgg ccaagccttt gtctcaagaa gaatccaccc
     4741 tcattgaaag agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg
     4801 ccagcgcagc tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta
     4861 ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca
     4921 acctgacttg tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac
     4981 ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg
     5041 atggacagcc gacggcagtt gggattcgtg aattgctgcc ctctggttat gcgtgggagg
     5101 gctaagtcga caatcaacct ctggattaca aaatttgtgg gttaacttgt ttattgcagc
     5161 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     5221 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggaattg
     5281 actcaaatga tgtcaattag tctatcagaa gctatctggt ctcccttccg ggggacaaga
     5341 catccctgtt taatatttaa acagcagtgt tcccaaactg ggttcttata tcccttgctc
     5401 tggtcaacca ggttgcaggg tttcctgtcc tcacaggaac gaagtcccta aagaaacagt
     5461 ggcagccagg tttagccccg gaattgactg gattcctttt ttagggccca ttggtatggc
     5521 tttttccccg tatcccccca ggtgtctgca ggctcaaaga gcagcgagaa gcgttcagag
     5581 gaaagcgatc ccgtgccacc ttccccgtgc ccgggctgtc cccgcacgct gccggctcgg
     5641 ggatgcgggg ggagcgccgg accggagcgg agccccgggc ggctcgctgc tgccccctag
     5701 cgggggaggg acgtaattac atccctgggg gctttggggg gggctgtccc tgatatctat
     5761 aacaagaaaa tatatatata ataagttatc acgtaagtag aacatgaaat aacaatataa
     5821 ttatcgtatg agttaaatct taaaagtcac gtaaaagata atcatgcgtc attttgactc
     5881 acgcggtcgt tatagttcaa aatcagtgac acttaccgca ttgacaagca cgcctcacgg
     5941 gagctccaag cggcgactga gatgtcctaa atgcacagcg acggattcgc gctatttaga
     6001 aagagagagc aatatttcaa gaatgcatgc gtcaatttta cgcagactat ctttctaggg
     6061 ttaatctagc tgcatcagga tcatatcgtc gggtcttttt tccggctcag tcatcgccca
     6121 agctggcgct atctgggcat cggggaggaa gaagcccgtg ccttttcccg cgaggttgaa
     6181 gcggcatgga aagagtttgc cgaggatgac tgctgctgca ttgacgttga gcgaaaacgc
     6241 acgtttacca tgatgattcg ggaaggtgtg gccatgcacg cctttaacgg tgaactgttc
     6301 gttcaggcca cctgggatac cagttcgtcg cggcttttcc ggacacagtt ccggatggtc
     6361 agcccgaagc gcatcagcaa cccgaacaat accggcgaca gccggaactg ccgtgccggt
     6421 gtgcagatta atgacagcgg tgcggcgctg ggatattacg tcagcgagga cgggtatcct
     6481 ggctggatgc cgcagaaatg gacatggata ccccgtgagt tacccggcgg gcgcgcttgg
     6541 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     6601 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     6661 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     6721 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     6781 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     6841 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     6901 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     6961 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     7021 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     7081 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     7141 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     7201 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     7261 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     7321 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     7381 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     7441 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     7501 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     7561 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     7621 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     7681 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     7741 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     7801 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     7861 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     7921 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     7981 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     8041 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     8101 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     8161 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     8221 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     8281 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     8341 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     8401 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     8461 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     8521 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaat
//
