LOCUS       sequence-348925-       10588 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10588)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10588)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3175
FEATURES             Location/Qualifiers
     source          1..10588
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3342..3366)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3374..3415
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3526..4114
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3579..3599)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3997..4008)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4186..4419
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4497..4618
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4534..4553)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4588..4607
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4658..4793
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4813..4832)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4814..4832)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4842..4859)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4842..4858)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4851..4873)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5000..5455
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5087..5106)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5297..5318
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5481..5585
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5586..5654,5655..6446)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5804..5823)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6617..7205
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7106..7125
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7359..7376
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7493..7514
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7529..7534,7535..7552,7553..7559)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7567..7583
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7572..7594
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7591..7607
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7591..7607
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7626..7646
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7628..7646
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7674..7900
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7901..8081
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8128..8253
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8746..8979
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9164..9208
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9439..9649
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9461..9657
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9508..9643
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9689..10291
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9689..9708)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10185..10205
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10334..10451
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcatgcgtcc aatgcgaatt tttgtgaatg atgaccgcca
     1261 tgtgatggca aagcattctt ccgtttatcc aacacaagag gagctggagg cagtccagaa
     1321 catggtgtcc cacacggagc gggcgctcaa agctgtgtcc gactggatag acgagcagga
     1381 aaagggtagc agcgagcagg cagagtccga taacatggat gtgcccccag aggacgacag
     1441 taaagaaggg gctggggaac agaagacgga gcacatgacc agaaccctgc ggggagtgat
     1501 gcgggtgggc ctggtggcaa agggcctcct actcaagggg gacttggatc tggagctggt
     1561 gctgctgtgt aaggagaagc ccacaaccgc cctcctggac aaggtggccg acaacctggc
     1621 catccagctt gctgctgtaa cagaagacaa gtacgaaata ctgcaatctg tcgacgatgc
     1681 tgcgattgtg ataaaaaaca caaaagagcc tccattgtcc ctgaccatcc acctgacatc
     1741 ccctgttgtc agagaagaaa tggagaaagt attagctgga gaaacgctat cagtcaacga
     1801 ccccccggac gttctggaca ggcagaaatg ccttgctgcc ttggcgtccc tccgacacgc
     1861 caagtggttc caggccagag ccaacgggct gaagtcttgt gtcattgtga tccgggtctt
     1921 gagggacctg tgcactcgcg tgcccacctg gggtcccctc cgaggctggc ctctcgagct
     1981 cctgtgtgag aaatccattg gcacggccaa cagaccgatg ggtgctggcg aggccctgcg
     2041 gagagtgctg gagtgcctgg cgtcgggcat cgtgatgcca gatggttctg gcatttatga
     2101 cccttgtgaa aaagaagcca ctgatgctat tgggcatcta gacagacagc aacgggaaga
     2161 tatcacacag agtgcgcagc acgcactgcg gctcgctgcc ttcggccagc tccataaagt
     2221 cctaggcatg gaccctctgc cttccaagat gcccaagaaa ccaaagaatg aaaacccagt
     2281 ggactacacc gttcagatcc caccaagcac cacctatgcc attacgccca tgaaacgccc
     2341 aatggaggag gacggggagg agaagtcgcc cagcaaaaag aagaagaaga ttcagaagaa
     2401 agaggagaag gcagagcccc cccaggctat gaatgccctg atgcggttga accagctgaa
     2461 gccagggctg cagtacaagc tggtgtccca gactgggccc gtccatgccc ccatctttac
     2521 catgtctgtg gaggttgatg gcaattcatt cgaggcctct gggccctcca aaaagacggc
     2581 caagctgcac gtggccgtta aggtgttaca ggacatgggc ttgccgacgg gtgctgaagg
     2641 cagggactcg agcaaggggg aggactcggc tgaggagacc gaggcgaagc cagcagtggt
     2701 ggcccctgcc ccagtggtag aagctgtctc cacccctagt gcggcctttc cctcagatgc
     2761 cactgccgag aacgtaaaac agcaggggcc gatcctgaca aagcacggca agaacccagt
     2821 catggagctg aacgagaaga ggcgtgggct caagtacgag ctcatctccg agaccggggg
     2881 cagccacgac aagcgcttcg tcatggaggt cgaagtggat ggacagaagt tccaaggtgc
     2941 tggttccaac aaaaaggtgg cgaaggccta cgctgctctt gctgccctag aaaagctttt
     3001 ccctgacacc cctctcgccc ttgatgccaa caaaaagaag agagccccag tacccgtcag
     3061 agggggaccg aaatttgctg ctaagccaca taaccctggc ttcggcatgg gaggccccat
     3121 gcacaacgaa gtgcccccac cccccaacct tcgagggcgg ggaagaggcg ggagcatccg
     3181 gggacgaggg cgcgggcgag gatttggtgg cgccaaccat ggaggctaca tgaatgccgg
     3241 tgctgggtat ggaagctatg ggtacggagg caactctgcg acagcaggct acagtgactt
     3301 tttcacagac tgctacggct atcatgattt tgggtcttcc tacccaactt tcttgtacaa
     3361 agtggttgat atcggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgtagta
     3421 atgaactagt ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
     3481 ggacgatatg agtattctcc accacagttg acgcgttaag tcgacaatca acctctggat
     3541 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     3601 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     3661 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     3721 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     3781 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     3841 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     3901 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     3961 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     4021 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     4081 acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca atgacttaca
     4141 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     4201 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     4261 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     4321 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     4381 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tacgtatagt agttcatgtc
     4441 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     4501 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     4561 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     4621 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     4681 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     4741 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     4801 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     4861 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     4921 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     4981 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     5041 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     5101 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     5161 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     5221 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     5281 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     5341 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     5401 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     5461 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     5521 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     5581 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     5641 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     5701 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     5761 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     5821 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     5881 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     5941 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     6001 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     6061 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     6121 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     6181 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     6241 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     6301 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     6361 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     6421 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     6481 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     6541 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     6601 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     6661 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     6721 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     6781 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     6841 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     6901 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     6961 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     7021 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     7081 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     7141 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     7201 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     7261 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     7321 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     7381 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     7441 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     7501 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     7561 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     7621 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc
     7681 ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
     7741 aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt
     7801 attaggaagg caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg
     7861 cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca
     7921 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     7981 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     8041 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     8101 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
     8161 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     8221 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     8281 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     8341 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     8401 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     8461 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     8521 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     8581 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     8641 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     8701 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     8761 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     8821 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     8881 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     8941 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     9001 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     9061 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     9121 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     9181 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     9241 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     9301 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     9361 cctcccaacc ccgaggggac ccatgcattg catctcaatt agtcagcaac caggtgtgga
     9421 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcgtctcaa ttagtcagca
     9481 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     9541 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     9601 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     9661 ctttctagag gtaccaccgg agcttaccat gaccgagtac aagcccacgg tgcgcctcgc
     9721 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     9781 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
     9841 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     9901 cgccgtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     9961 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
    10021 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
    10081 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
    10141 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
    10201 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
    10261 gtgcatgacc cgcaagcccg gtgccagtta acgaattcta gatcttgaga caaatggcag
    10321 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
    10381 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
    10441 ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggcgc cggctcgagg
    10501 cctgcaggtg caaagatgga taaagtttta aacagagagg aatctttgca gctaatggac
    10561 cttctaggtc ttgaaaggag tgggaatt
//
