LOCUS       sequence-348926-       10615 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10615)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3176
FEATURES             Location/Qualifiers
     source          1..10615
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3369..3393)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3401..3442
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3553..4141
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3606..3626)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4024..4035)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4213..4446
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4524..4645
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4561..4580)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4615..4634
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4685..4820
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4840..4859)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4841..4859)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4869..4886)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4869..4885)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4878..4900)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5027..5482
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5114..5133)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5324..5345
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5508..5612
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5613..5681,5682..6473)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5831..5850)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6644..7232
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7133..7152
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7386..7403
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7520..7541
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7556..7561,7562..7579,7580..7586)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7594..7610
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7599..7621
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7618..7634
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7618..7634
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7653..7673
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7655..7673
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7701..7927
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7928..8108
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8155..8280
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8773..9006
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9191..9235
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9466..9676
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9488..9684
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9535..9670
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9716..10318
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9716..9735)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10212..10232
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10361..10478
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcatggaagc acgttctatg ctggttccac cccaggcatc
     1261 tgtgtgcttc gaggatgtgg ctatggcatt cacacaggag gagtgggaac agctggacct
     1321 ggcccagagg acactgtacc gagaggtgac actggagacc tgggagcata ttgtctccct
     1381 ggggcttttc ctttccaaat ctgatgtgat ctctcagctg gagcaagaag aggacctgtg
     1441 cagggcagag caggaggccc cccgagactg gaaagctacc cttgaggaga ataggttgaa
     1501 ttctgaaaaa gatcgagcta gggaagaact atcccaccac gtggaagtgt acaggagtgg
     1561 accggaggag ccaccctctt tggtattagg aaaagtgcaa gatcagagca accagttaag
     1621 ggaacaccag gagaactcct tgaggttcat ggtactcacc tcagagagac tgtttgctca
     1681 aagggaacat tgtgagcttg aacttggggg aggttattct ctaccttcta ctttaagcct
     1741 tctacctaca acattaccta caagtacagg tttccctaag cccaactcac aagttaaaga
     1801 gttgaaacaa aattcagctt tcattaatca tgagaaaaat ggagcagatg ggaagcactg
     1861 tgagagtcat cagtgtgcta gagctttctg tcagagtatt tacttgagta aacttggaaa
     1921 cgttgaaaca ggaaagaaaa acccttatga atatattgtc agtggtgact ctctcaacta
     1981 tggttcctcc ctttgttttc atggtagaac tttttcagtg aagaaaagtg atgactgtaa
     2041 ggattatgga aacctcttca gtcacagtgt gtctctgaat gaacagaagc cagtgcattt
     2101 tgggaaaagt cagtatgagt gtgatgagtg cagggaaacc tgttctgaga gtctgtgcct
     2161 tgtacaaaca gaaagaagtg gccctggaga gacccccttc agatgtgagg aacgctgtgc
     2221 tgccttcccc atggcctcat ctttttctga ctgtaacatc attcagacta cagagaagcc
     2281 atctgtgtgt aatcagtgtg gaaaatcttt cagctgttgt aagctcatac accagagaac
     2341 acacactgga gaaaagccct tcgaatgtac tcagtgtggg aaatctttta gccagagcta
     2401 tgaccttgtc atacatcaga ggacacacac tggagagaag ccctatgagt gtgacctgtg
     2461 tgggaaatcc ttcacccaga gatccaaact tattacacat cagcgaattc acactggaga
     2521 aaaaccgtat cagtgtattg aatgcagaaa atccttcagg tggaactcta acctcattgt
     2581 acatcagaga attcatactg gagagaaacc gtatgagtgc actcactgtg gaaagtcctt
     2641 cagccaaagc tatgagttag ttacacataa aagaacgcac actggagaaa aacccttcaa
     2701 atgtactcag tgtgggaaat ctttcagcca gaagtatgac cttgttgtac atcagaggac
     2761 acacactgga gagaagccct atgagtgcaa cctgtgtggg aaatccttct cccagagttc
     2821 caaacttatt acgcatcagc gaattcacac tggagaaaaa ccgtatcagt gtattgaatg
     2881 tgggaaatcc ttcagatgga actctaacct cgtcatacat cagagaattc atactggaga
     2941 gaaaccgtac gattgcactc actgtggaaa gtccttcagc caaagctatc agttagttgc
     3001 acataaaaga actcacactg gagaaaagcc ctatgaatgt aacgagtgtg gaaaagcctt
     3061 caatcgaagc actcagctca tcaggcatct gcaaattcac actggggaga agccgtacaa
     3121 atgcaatcag tgcaataaag cctttgcaag gagctcctac cttgtgatgc atcagagaac
     3181 tcacactggt gagaaacctt ttgagtgtag tcagtgtggg aaagcctttt cagggagctc
     3241 taaccttctt tcccatcaca gaattcattc tggagagaaa ccctatgaat gtagtgactg
     3301 tgggaaatcc ttccggcagc aatctcaact tgtagtgcat cggcggacac atactggaga
     3361 gaaaccttac ccaactttct tgtacaaagt ggttgatatc ggtaagccta tccctaaccc
     3421 tctcctcggt ctcgattcta cgtagtaatg aactagtccg taacttgaaa gtatttcgat
     3481 ttcttggctt tatatatctt gtggaaagga cgacgctcac cagcagtcct taggcttacg
     3541 cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     3601 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     3661 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     3721 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     3781 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     3841 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     3901 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     3961 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     4021 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     4081 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     4141 cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     4201 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     4261 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     4321 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     4381 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     4441 ctagcagtac gtatagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     4501 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     4561 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     4621 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     4681 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     4741 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     4801 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
     4861 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     4921 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     4981 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
     5041 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     5101 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     5161 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     5221 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     5281 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     5341 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     5401 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     5461 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     5521 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     5581 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     5641 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     5701 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     5761 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     5821 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     5881 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     5941 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     6001 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     6061 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     6121 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     6181 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     6241 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     6301 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     6361 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     6421 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     6481 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     6541 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     6601 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     6661 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     6721 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     6781 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     6841 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     6901 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     6961 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     7021 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     7081 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     7141 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     7201 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     7261 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     7321 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     7381 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     7441 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     7501 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     7561 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     7621 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
     7681 agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt
     7741 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt
     7801 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat
     7861 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata
     7921 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     7981 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     8041 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     8101 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     8161 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     8221 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     8281 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     8341 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     8401 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     8461 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     8521 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     8581 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     8641 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     8701 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     8761 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     8821 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     8881 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     8941 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     9001 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     9061 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     9121 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     9181 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     9241 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     9301 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     9361 atattcacca ttatcgtttc agacccacct cccaaccccg aggggaccca tgcattgcat
     9421 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     9481 caaagcatgc gtctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     9541 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     9601 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     9661 tttggaggcc taggcttttg caaaaagctt tctagaggta ccaccggagc ttaccatgac
     9721 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     9781 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     9841 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     9901 caaggtgtgg gtcgcggacg acggcgccgc cgtggcggtc tggaccacgc cggagagcgt
     9961 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
    10021 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
    10081 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
    10141 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
    10201 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
    10261 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg ccagttaacg
    10321 aattctagat cttgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
    10381 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
    10441 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
    10501 gagatccact ttggcgccgg ctcgaggcct gcaggtgcaa agatggataa agttttaaac
    10561 agagaggaat ctttgcagct aatggacctt ctaggtcttg aaaggagtgg gaatt
//
