LOCUS       sequence-348928-       10213 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10213)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10213)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3177
FEATURES             Location/Qualifiers
     source          1..10213
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2967..2991)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2999..3040
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3151..3739
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3204..3224)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3622..3633)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3811..4044
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4122..4243
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4159..4178)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4213..4232
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4283..4418
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4438..4457)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4439..4457)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4467..4484)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4467..4483)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4476..4498)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4625..5080
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4712..4731)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4922..4943
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5106..5210
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5211..5279,5280..6071)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5429..5448)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6242..6830
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6731..6750
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6984..7001
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7118..7139
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7154..7159,7160..7177,7178..7184)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7192..7208
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7197..7219
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7216..7232
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7216..7232
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7251..7271
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7253..7271
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7299..7525
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7526..7706
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7753..7878
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8371..8604
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8789..8833
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9064..9274
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9086..9282
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9133..9268
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9314..9916
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9314..9333)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9810..9830
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9959..10076
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagcag gcaccatgga ggtgaatgca ggaggtgtga ttgcctatat
     1261 cagttcttcc agctcagcct caagccctgc ctcttgtcac agtgagggtt ctgagaatag
     1321 tttccagtcc tcctcctctt ctgttccatc ttctccaaat agctctaatt ctgataccaa
     1381 tggtaatccc aagaatggtg atctcgccaa tattgaaggc atcttgaaga atgatcgaat
     1441 agattgttct atgaaaacaa gcaaatcgag tgcacctggg atgacaaaaa gtcatagtgg
     1501 tgtgacaaaa tttagtggca tggttctact gtgtaaagtc tgtggggatg tggcgtcagg
     1561 attccactat ggagttcatg cttgcgaagg ctgtaagggt ttctttcgga gaagtattca
     1621 acaaaacatc cagtacaaga agtgcctgaa gaatgaaaac tgttctataa tgagaatgaa
     1681 taggaacaga tgtcagcaat gtcgcttcaa aaagtgtctg tctgttggaa tgtcaagaga
     1741 tgctgttcgg tttggtcgta ttcctaagcg tgaaaaacag aggatgctaa ttgaaatgca
     1801 aagtgcaatg aagaccatga tgaacagcca gttcagtggt cacttgcaaa atgacacatt
     1861 agtagaacat catgaacaga cagccttgcc agcccaggaa cagctgcgac ccaagcccca
     1921 actggagcaa gaaaacatca aaagctcttc tcctccatct tctgattttg caaaggaaga
     1981 agtgattggc atggtgacca gagctcacaa ggataccttt atgtataatc aagagcagca
     2041 agaaaactca gctgagagca tgcagcccca gagaggagaa cggattccca agaacatgga
     2101 gcaatataat ttaaatcatg atcattgcgg caatgggctt agcagccatt ttccctgtag
     2161 tgagagccag cagcatctca atggacagtt caaagggagg aatataatgc attacccaaa
     2221 tggtcatgcc atttgtattg caaatggaca ttgtatgaac ttctccaatg cttatactca
     2281 aagagtatgt gatagagttc cgatagatgg attttctcag aatgagaaca agaatagtta
     2341 cctgtgcaac actggaggaa gaatgcatct ggtttgtcca atgagtaagt ctccatatgt
     2401 ggaccctcat aaatcaggac atgaaatctg ggaagaattt tcgatgagct tcactccagc
     2461 agtgaaagaa gtggtggaat ttgcaaagcg tattcctggg ttcagagatc tctctcagca
     2521 tgaccaggtc aaccttttaa aggctgggac ttttgaggtt ttaatggtac ggttcgcatc
     2581 attatttgat gcaaaggaac gtactgtcac ctttttaagt ggaaagaaat atagtgtgga
     2641 tgatttacac tcaatgggag caggggatct gctaaactct atgtttgaat ttagtgagaa
     2701 gctaaatgcc ctccaactta gtgatgaaga gatgagtttg tttacagctg ttgtcctggt
     2761 atctgcagat cgatctggaa tagaaaacgt caactctgtg gaggctttgc aggaaactct
     2821 cattcgtgca ctaaggacct taataatgaa aaaccatcca aatgaggcct ctatttttac
     2881 aaaactgctt ctaaagttgc cagatcttcg atctttaaac aacatgcact ctgaggagct
     2941 cttggccttt aaagttcacc cttaagaccc agctttcttg tacaaagtgg ttgatatcgg
     3001 taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgaa ctagtccgta
     3061 acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg agacgttcag
     3121 tggaatcgct atcccacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa
     3181 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     3241 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     3301 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     3361 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     3421 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     3481 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     3541 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     3601 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     3661 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     3721 ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc
     3781 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     3841 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     3901 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     3961 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     4021 agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt
     4081 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     4141 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     4201 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     4261 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     4321 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     4381 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     4441 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     4501 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     4561 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     4621 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     4681 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     4741 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     4801 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     4861 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     4921 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     4981 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     5041 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     5101 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     5161 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     5221 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     5281 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     5341 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     5401 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     5461 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     5521 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     5581 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     5641 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     5701 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     5761 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     5821 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     5881 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     5941 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     6001 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     6061 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     6121 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     6181 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     6241 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     6301 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     6361 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     6421 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     6481 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     6541 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     6601 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     6661 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     6721 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     6781 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     6841 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     6901 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     6961 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     7021 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     7081 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     7141 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     7201 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
     7261 ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt
     7321 gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca
     7381 ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca
     7441 gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt
     7501 taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga
     7561 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     7621 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     7681 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     7741 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     7801 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     7861 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     7921 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     7981 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     8041 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     8101 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     8161 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     8221 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     8281 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     8341 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     8401 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     8461 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     8521 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     8581 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     8641 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     8701 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     8761 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     8821 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     8881 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     8941 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     9001 gggacccatg cattgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     9061 ccagcaggca gaagtatgca aagcatgcgt ctcaattagt cagcaaccat agtcccgccc
     9121 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     9181 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     9241 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctttc tagaggtacc
     9301 accggagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     9361 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     9421 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     9481 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgccg tggcggtctg
     9541 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     9601 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     9661 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     9721 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     9781 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     9841 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     9901 gcccggtgcc agttaacgaa ttctagatct tgagacaaat ggcagtattc atccacaatt
     9961 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
    10021 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
    10081 tttattacag ggacagcaga gatccacttt ggcgccggct cgaggcctgc aggtgcaaag
    10141 atggataaag ttttaaacag agaggaatct ttgcagctaa tggaccttct aggtcttgaa
    10201 aggagtggga att
//
