LOCUS       sequence-348943-        4417 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid for knock-in of MT-C34 peptide into exon-2 of CXCR4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Maslennikova A, Kruglova N, Kalinichenko S, Komkov D, Shepelev M, 
            Golubev D, Siniavin A, Vzorov A, Filatov A, Mazurov D
  TITLE     Engineering T-Cell Resistance to HIV-1 Infection via Knock-In of 
            Peptides from the Heptad Repeat 2 Domain of gp41.
  JOURNAL   mBio. 2022 Jan 25:e0358921. doi: 10.1128/mbio.03589-21.
  PUBMED    35073736
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio. 2022 
            Jan 25:e0358921. doi: 10.1128/mbio.03589-21."
COMMENT     Sequence Label: pJet-gR-X4ex2-DTS
FEATURES             Location/Qualifiers
     source          1..4417
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             632..688
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     polyA_signal    946..994
                     /note="synthetic polyadenylation signal"
     primer_bind     1523..1543
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(join(1945..1951,1952..1969,1970..1975))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    1990..2011
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2131..2148)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2302..2890)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2382..2401)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3061..3852,3853..3921))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3684..3703
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3922..4026)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4094..4112
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 taatacgact cactataggg agagcggccg aattcacttc agataactac accgaggtcg
       61 agtttttcag caagattttg cgctttagga gaatgagtct ttgcaacgcc cccgccctcc
      121 ccccgtgatc ctcccttctc ccctcttccc tccctgggcg aaaaacttct tacaaaaagt
      181 taatcactgc ccctcctagc agcacccacc ccacccccca cgccgcctgg gagtggcctc
      241 tttgtgtgta tttttttttc ctcctaagga aggttttttt tcttccctct agtgggcggg
      301 gcagaggagt tagccaagat gtgactttga aaccctcagc gtctcagtgc ccttttgttc
      361 taaacaaaga attttgtaat tggttctacc aaagaaggat ataatgaagt cactatggga
      421 aaagatgggg aggagagttg taggattcta cattaattct cttgtgccct tagcccacta
      481 cttcagaatt tcctgaagaa agcaagcctg aattggtttt ttaaattgct ttaaaaattt
      541 tttttaactg ggttaatgct tgctgaattg gaagtgaatg tccattcctt tgcctctttt
      601 gcagatatac acttcagata acggatccgg cgcaacaaac ttctctctgc tgaaacaagc
      661 cggagatgtc gaagagaatc ctggaccgaa acgcttcctc ttcctcctac tcaccatcag
      721 cctcctggtt atggtacaga tacaaactgg actctcaatg acctggatgg agtgggaccg
      781 ggagatcaat aactacacct cactgatcca cagcctgatc gaggagtccc agaaccagca
      841 ggagaagaac gagcaggagc tcctgtcagc atccagcaac ataagcggag gcattttcct
      901 tttcttcgtg gccaatgcca taatccacct cttctgcttc agttgaataa aatatcttta
      961 ttttcattac atctgtgtgt tggttttttg tgtgaggaaa tgggctcagg ggactatgac
     1021 tccatgaagg aaccctgttt ccgtgaagaa aatgctaatt taataaaatc ttcctgccca
     1081 ccatctactc catcatcttc ttaactggca ttgtgggcaa tggattggtc atcctggtca
     1141 tgggttacca gaagaaactg agaagcatga cggacaagta caggctgcac ctgtcagtgg
     1201 ccgacctcct ctttgtcatc acgcttccct tctgggcagt tgatgccgtg gcaaactggt
     1261 actttgggaa cttcctatgc aaggcagtcc atgtcatcta cacagtcaac ctctacagca
     1321 gtgtcctcat cctggccttc atcagtctgg accgctacct ggccatcgtc cacgccacca
     1381 acagtcagag gccaaggaag ctgttggctg aaaaggtggt ctatgttggc gtctggatcc
     1441 ctgccctcct gctgactatt cccgatatct ttctagatgc tttgcatact tctgcctgct
     1501 ggggagcctg gggactttcc acaccctaac tgacacacat tccacagcat ggaaaatcga
     1561 tgttcttctt ttattctctc aagattttca ggctgtatat taaaacttat attaagaact
     1621 atgctaacca cctcatcagg aaccgttgta ggtggcgtgg gttttcttgg caatcgactc
     1681 tcatgaaaac tacgagctaa atattcaata tgttcctctt gaccaacttt attctgcatt
     1741 ttttttgaac gaggtttaga gcaagcttca ggaaactgag acaggaattt tattaaaaat
     1801 ttaaattttg aagaaagttc agggttaata gcatccattt tttgctttgc aagttcctca
     1861 gcattcttaa caaaagacgt ctcttttgac atgtttaaag tttaaacctc ctgtgtgaaa
     1921 ttattatccg ctcataattc cacacattat acgagccgga agcataaagt gtaaagcctg
     1981 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc caattgcttt
     2041 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     2101 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     2161 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     2221 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     2281 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     2341 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     2401 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     2461 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     2521 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     2581 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     2641 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     2701 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     2761 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     2821 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     2881 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     2941 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     3001 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     3061 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     3121 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     3181 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     3241 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     3301 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     3361 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     3421 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     3481 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     3541 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     3601 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     3661 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     3721 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     3781 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     3841 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     3901 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     3961 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     4021 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     4081 attaacctat aaaaataggc gtatcacgag gccgcccctg cagccgaatt atattatttt
     4141 tgccaaataa tttttaacaa aagctctgaa gtcttcttca tttaaattct tagatgatac
     4201 ttcatctgga aaattgtccc aattagtagc atcacgctgt gagtaagttc taaaccattt
     4261 ttttattgtt gtattatctc taatcttact actcgatgag ttttcggtat tatctctatt
     4321 tttaacttgg agcaggttcc attcattgtt tttttcatca tagtgaataa aatcaactgc
     4381 tttaacactt gtgcctgaac accatatcca tccggcg
//
