LOCUS       sequence-348963-        9849 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the firefly luciferase gene, E2A, eGFP, F2A, and the 
            puromycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9849)
  AUTHORS   Mustafa EH, Laven-Law G, Kikhtyak Z, Nguyen V, Ali S, Pace AA, Iggo 
            R, Kebede A, Noll B, Wang S, Winter JM, Dwyer AR, Tilley WD, Hickey 
            TE
  TITLE     Selective inhibition of CDK9 in triple negative breast cancer.
  JOURNAL   Oncogene. 2023 Nov 24. doi: 10.1038/s41388-023-02892-3.
  PUBMED    38001268
REFERENCE   2  (bases 1 to 9849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncogene. 
            2023 Nov 24. doi: 10.1038/s41388-023-02892-3."
COMMENT     Sequence Label: pDRM210 LGP (Luciferase GFP Puro)
FEATURES             Location/Qualifiers
     source          1..9849
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    96..117
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(129..134,135..152,153..159)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    167..183
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     172..194
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     191..207
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     191..207
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     226..246
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        228..246
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        274..500
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             501..681
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    728..850
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1343..1576
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1761..1805
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2072..2189
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      2791..2800
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2797..4446
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(2863..2881)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     CDS             4456..4515
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             join(4519..4521,4522..4524,4525..5235)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4519..4521,4522..4524,4525..5235)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4564..4585)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4825..4844)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5172..5193
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5362..5961
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5362..5381)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5858..5878
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5971..6559
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6024..6044)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6442..6453)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6646..6879
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6951..7072
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6988..7007)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7042..7061
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7112..7247
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7267..7286)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7268..7286)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7296..7313)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7296..7312)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7305..7327)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7454..7907
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7541..7560)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7749..7770
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7933..8037
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8038..8106,8107..8898)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8256..8275)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9069..9657
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9558..9577
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9811..9828
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
       61 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
      121 gcccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
      181 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
      241 taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt
      301 cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg
      361 catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg
      421 gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt
      481 gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct
      541 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
      601 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
      661 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
      721 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
      781 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
      841 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
      901 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
      961 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
     1021 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
     1081 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
     1141 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct
     1201 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
     1261 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
     1321 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
     1381 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
     1441 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
     1501 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
     1561 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
     1621 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
     1681 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
     1741 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
     1801 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
     1861 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
     1921 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg
     1981 acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat
     2041 tagtgaacgg atctcgacgg tatcggttaa cttttaaaag aaaagggggg attggggggt
     2101 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     2161 aaaaacaaat tacaaaaatt caaaatttta tcgatcacga gactagcctc gaggatatcg
     2221 agatcgatta gtccaatttg ttaaagacag gatatcagtg gtccaggctc tagttttgac
     2281 tcaacaatat caccagctga agcctataga gtacgagcca tagatagaat aaaagatttt
     2341 atttagtctc cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagg
     2401 atcaaggtta ggaacagaga gacagcagaa tatgggccaa acaggatatc tgtggtaagc
     2461 agttcctgcc ccggctcagg gccaagaaca gttggaacag cagaatatgg gccaaacagg
     2521 atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg
     2581 cggtcccgcc ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac
     2641 ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg
     2701 cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg gcgcgatcga
     2761 tgaattcgag ctcggtaccc ggggatccaa gccaccatgg aagacgccaa aaacataaag
     2821 aaaggcccgg cgccattcta tccgctggaa gatggaaccg ctggagagca actgcataag
     2881 gctatgaaga gatacgccct ggttcctgga acaattgctt ttacagatgc acatatcgag
     2941 gtggacatca cttacgctga gtacttcgaa atgtccgttc ggttggcaga agctatgaaa
     3001 cgatatgggc tgaatacaaa tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc
     3061 tttatgccgg tgttgggcgc gttatttatc ggagttgcag ttgcgcccgc gaacgacatt
     3121 tataatgaac gtgaattgct caacagtatg ggcatttcgc agcctaccgt ggtgttcgtt
     3181 tccaaaaagg ggttgcaaaa aattttgaac gtgcaaaaaa agctcccaat catccaaaaa
     3241 attattatca tggattctaa aacggattac cagggatttc agtcgatgta cacgttcgtc
     3301 acatctcatc tacctcccgg ttttaatgaa tacgattttg tgccagagtc cttcgatagg
     3361 gacaagacaa ttgcactgat catgaactcc tctggatcta ctggtctgcc taaaggtgtc
     3421 gctctgcctc atagaactgc ctgcgtgaga ttctcgcatg ccagagatcc tatttttggc
     3481 aatcaaatca ttccggatac tgcgatttta agtgttgttc cattccatca cggttttgga
     3541 atgtttacta cactcggata tttgatatgt ggatttcgag tcgtcttaat gtatagattt
     3601 gaagaagagc tgtttctgag gagccttcag gattacaaga ttcaaagtgc gctgctggtg
     3661 ccaaccctat tctccttctt cgccaaaagc actctgattg acaaatacga tttatctaat
     3721 ttacacgaaa ttgcttctgg tggcgctccc ctctctaagg aagtcgggga agcggttgcc
     3781 aagaggttcc atctgccagg tatcaggcaa ggatatgggc tcactgagac tacatcagct
     3841 attctgatta cacccgaggg ggatgataaa ccgggcgcgg tcggtaaagt tgttccattt
     3901 tttgaagcga aggttgtgga tctggatacc gggaaaacgc tgggcgttaa tcaaagaggc
     3961 gaactgtgtg tgagaggtcc tatgattatg tccggttatg taaacaatcc ggaagcgacc
     4021 aacgccttga ttgacaagga tggatggcta cattctggag acatagctta ctgggacgaa
     4081 gacgaacact tcttcatcgt tgaccgcctg aagtctctga ttaagtacaa aggctatcag
     4141 gtggctcccg ctgaattgga atccatcttg ctccaacacc ccaacatctt cgacgcaggt
     4201 gtcgcaggtc ttcccgacga tgacgccggt gaacttcccg ccgccgttgt tgttttggag
     4261 cacggaaaga cgatgacgga aaaagagatc gtggattacg tcgccagtca agtaacaacc
     4321 gcgaaaaagt tgcgcggagg agttgtgttt gtggacgaag taccgaaagg tcttaccgga
     4381 aaactcgacg caagaaaaat cagagagatc ctcataaagg ccaagaaggg cggaaagatc
     4441 gccgtggcta gcggacagtg tactaattat gctctcttga aattggctgg agatgttgag
     4501 agcaaccctg gacctcatat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     4561 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     4621 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     4681 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     4741 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     4801 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     4861 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     4921 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     4981 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     5041 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     5101 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     5161 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     5221 gacgagctgt acaagtacag cggtcgcgac tctagagtcg acggttctgg taagcaaact
     5281 ttgaattttg accttcttaa gcttgcggga gacgtcgagt ccaaccccgg gcccgcatgc
     5341 aagcttcagc tgaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc
     5401 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     5461 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     5521 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     5581 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     5641 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     5701 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     5761 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     5821 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     5881 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     5941 acccgcaagc ccggtgcctg actagtcgac aatcaacctc tggattacaa aatttgtgaa
     6001 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     6061 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     6121 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     6181 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     6241 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     6301 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     6361 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     6421 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     6481 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     6541 ctttgggccg cctccccgcc tggaattcga gctcggtacc tttaagacca atgacttaca
     6601 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     6661 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     6721 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     6781 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     6841 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     6901 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     6961 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     7021 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     7081 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     7141 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     7201 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
     7261 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     7321 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     7381 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     7441 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     7501 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     7561 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     7621 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     7681 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggaccacgtt
     7741 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     7801 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     7861 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
     7921 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     7981 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     8041 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     8101 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     8161 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     8221 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     8281 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     8341 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     8401 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     8461 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     8521 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     8581 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     8641 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     8701 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     8761 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     8821 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     8881 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     8941 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     9001 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     9061 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     9121 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     9181 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     9241 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     9301 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     9361 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     9421 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     9481 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     9541 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     9601 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     9661 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     9721 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     9781 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     9841 cgcccaata
//
