LOCUS       sequence-348988-        3225 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Dudley QM, Jo S, Guerrero DAS, Chhetry M, Smedley MA, Harwood WA, 
            Sherden NH, O'Connor SE, Caputi L, Patron NJ
  TITLE     Reconstitution of monoterpene indole alkaloid biosynthesis in genome
            engineered Nicotiana benthamiana.
  JOURNAL   Commun Biol. 2022 Sep 10;5(1):949. doi: 10.1038/s42003-022-03904-w.
  PUBMED    36088516
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-09-10"; volume: "5(1):949. doi"; pages: " 
            10.1038/s42003-022-03904-w"
COMMENT     Sequence Label: pEPQD0CM0060
FEATURES             Location/Qualifiers
     source          1..3225
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1206..1263
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1287..1304)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1458..2046)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1538..1557)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2229..2323)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2344..3003)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2918..2937
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     3165..3183
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(join(3187..3225,1..5))
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 atttctggaa ttcggtctca aatgagttgg tggtggaaga ggtccattgg tgctggaaag
       61 aacttaccaa accagaacaa agaaaatgga gtttgtaaaa gctacaagag tgtggcacta
      121 gtagtaggag tcaccggcat tgttggcagt agtttagccg aagttctaaa gctaccagac
      181 acccctggag gtccctggaa ggtatacggc gtggcaaggc gcccatgccc tgtatggcta
      241 gccaaaaaac cagttgagta catccaatgt gatgtctcag ataatcaaga aacaatttct
      301 aaattatctc cacttaagga cataactcat atattctatg tttcttggat tggatcagaa
      361 gattgccaaa caaatgctac aatgttcaaa aacatcctta attcagtgat tcctaatgct
      421 tcaaatctcc aacatgtgtg tctccaaaca ggaatcaaac attattttgg tatttttgaa
      481 gaaggttcta aagttgtacc acatgattcg ccttttaccg aggatttacc taggctaaat
      541 gtcccaaatt tctaccatga tcttgaagat atactgtatg aagaaactgg caagaacaat
      601 ttaacatggt ctgtccatag accagctctg gttttcgggt tttccccatg tagtatgatg
      661 aacattgtca gtacattatg cgtttacgcc acaatttgta aacatgaaaa taaggccttg
      721 gtttatccag gtagtaaaaa ttcatggaat tgttatgctg atgctgttga tgcagattta
      781 gtagccgagc acgaaatttg ggcagcagtt gatcctaagg ctaaaaatca agtattgaat
      841 tgtaacaatg gggatgtttt taaatggaaa catatttgga agaaattagc agaggaattt
      901 gggattgaaa tggtgggata tgttgaaggt aaagaacaag ttagtttggc tgagttaatg
      961 aaggataaag atcaagtttg ggatgaaatt gttaagaaga acaatttagt acctactaaa
     1021 ttgaaagaaa ttgctgcctt ttggtttgct gatattgctt tttgttctga gaatttgatt
     1081 agtagtatga acaaaagtaa ggaattaggg ttcttgggtt ttaggaattc gatgaaatcg
     1141 tttgtttctt gtattgataa gatgagggat tataggttta ttccttaggc ttcgagaccc
     1201 tgcagtccgg caaaaaaggg caaggtgtca ccaccctgcc ctttttcttt aaaaccgaaa
     1261 agattacttc gcgttatgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     1321 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     1381 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     1441 gccgcgttgc tggcgttttt ccacaggctc cgcccccctg acgagcatca caaaaatcga
     1501 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     1561 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     1621 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     1681 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     1741 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     1801 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     1861 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     1921 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     1981 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     2041 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     2101 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     2161 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagctcg
     2221 aggcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc
     2281 agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc
     2341 aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc
     2401 gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt
     2461 gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt
     2521 ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat
     2581 attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg
     2641 cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa
     2701 cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag
     2761 ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat
     2821 gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt
     2881 aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa
     2941 atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc
     3001 cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga
     3061 tcttatttca ttatggtgaa agttggaacc tcttacgtgc ccgatcaact cgagtgccac
     3121 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     3181 ggcagaattt cagataaaaa aaatccttag ctttcgctaa ggatg
//
