LOCUS       sequence-348989-        2541 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Dudley QM, Jo S, Guerrero DAS, Chhetry M, Smedley MA, Harwood WA, 
            Sherden NH, O'Connor SE, Caputi L, Patron NJ
  TITLE     Reconstitution of monoterpene indole alkaloid biosynthesis in genome
            engineered Nicotiana benthamiana.
  JOURNAL   Commun Biol. 2022 Sep 10;5(1):949. doi: 10.1038/s42003-022-03904-w.
  PUBMED    36088516
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-09-10"; volume: "5(1):949. doi"; pages: " 
            10.1038/s42003-022-03904-w"
COMMENT     Sequence Label: pEPQD0CM0068
FEATURES             Location/Qualifiers
     source          1..2541
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     567..586
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     814..832
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(836..879)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      1396..1453
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1477..1494)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1648..2236)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1728..1747)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2419..2513)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2534..2541,1..652))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc
       61 catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg
      121 tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta
      181 aatcaaaact ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa
      241 accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt
      301 gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt
      361 gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt
      421 tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg
      481 ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct
      541 gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac
      601 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt
      661 ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat
      721 tatggtgaaa gttggaacct cttacgtgcc cgatcaactc gagtgccacc tgacgtctaa
      781 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gcagaatttc
      841 agataaaaaa aatccttagc tttcgctaag gatgatttct ggaattcggt ctcaaatggc
      901 ttcgaagctt gaaatagaaa ttgagttgaa aagtgatgta gaaaagatgt ggaaacactt
      961 caaagaattc accaaattat tccccaaggc ttgtcctcat ctttacgaga agattgatgt
     1021 gatcgaggga gatgggatat cagttggaac aatctttgtc agtaccctga aaccaacaga
     1081 gttgaacccc gtggttatgg tgacgaagga gaagattgat tttttagatg atgagaacaa
     1141 aatgttgagg tatagttaca tggaagggga gatattaaag aattacaaga atttgagggg
     1201 cacagtgcat atgagcagca gcaaaagtgg tggaactata ttcaaatatt caggagaatt
     1261 cgaaaaggca aatgaacaag tccctgatcc tgttttcttc aaagatttta tggtcatagt
     1321 tttccaaggt ttggatgatt atattctcaa gggcatgaac catacatgtc aaaattaagc
     1381 ttcgagaccc tgcagtccgg caaaaaaggg caaggtgtca ccaccctgcc ctttttcttt
     1441 aaaaccgaaa agattacttc gcgttatgca ggcttcctcg ctcactgact cgctgcgctc
     1501 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     1561 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     1621 ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc cgcccccctg acgagcatca
     1681 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     1741 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     1801 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     1861 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     1921 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     1981 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     2041 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     2101 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     2161 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     2221 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     2281 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     2341 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     2401 tgacagctcg aggcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct
     2461 gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga
     2521 gctcgatatc aaattacgcc c
//
