LOCUS       sequence-348991-        3606 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Dudley QM, Jo S, Guerrero DAS, Chhetry M, Smedley MA, Harwood WA, 
            Sherden NH, O'Connor SE, Caputi L, Patron NJ
  TITLE     Reconstitution of monoterpene indole alkaloid biosynthesis in genome
            engineered Nicotiana benthamiana.
  JOURNAL   Commun Biol. 2022 Sep 10;5(1):949. doi: 10.1038/s42003-022-03904-w.
  PUBMED    36088516
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-09-10"; volume: "5(1):949. doi"; pages: " 
            10.1038/s42003-022-03904-w"
COMMENT     Sequence Label: pEPQD0CM0061
FEATURES             Location/Qualifiers
     source          1..3606
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1360..1417
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1441..1458)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1612..2200)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1692..1711)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2383..2477)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2498..3157)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3072..3091
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     3319..3337
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(3341..3384)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 cgggccgatc gtatggctcc agctcggctc aattaatact atggttgttc aaaatgcagt
       61 ttccgccgcc gagcttttta agaaacacga tgtccccttt tgtgaccgaa aagtccctga
      121 tactctcact gccttcaatt tcaaccaggg ctccctgggt atgaatactt acggcggaca
      181 ttggcgagtg cttagacgcc tctgttccat ggagtttttg gtaaataaac gtatgaacga
      241 aactacagat ctaagaagga gaattgagga caatatggtc cgttggattg aagaagattc
      301 attggcatct aaagcacaag gaggaaccgg agcggttcaa ttgagcaggt ttctttttct
      361 aatggcgttt aatttggtgg gtaaccttat gttatcgaga gatttaatgg ataataagga
      421 tccagaagga agggaatttt ttgattgtat gaatgagata ttggaattgg ctggtactcc
      481 taatattgct gattttttgc ctttgttgaa gaaattggat ccacttggaa tgaagaaaag
      541 gatggttgat aatatgtcta gaactatgaa aatttcttca aagtttgttc aagaaagact
      601 tgataataga aaagctggaa agattaatga aaagaaagat ttcttggatg ttatgcttga
      661 ataccaaggt gatggtaaag atggtcccga taaattcact gagcaacatg tcaacattgt
      721 catcatggaa atgttttttg caggatcaga gacaacaagt atcagcattg aatggggatt
      781 cacagaactt ctccgaaacc cacacgcatt caagaaagta agggaagaga tcgacagagt
      841 agtgggagta aacagaatgg tcgaagagag cgacatggag aatttgccgt atttgcaagc
      901 agttgtaaag gaaacgctta gattgcatcc ggcacttcca atgcttctac caagaaacac
      961 aatggaggac actgaataca tgggatacct cattcctaaa ggcacacaag ttttcgtgaa
     1021 tgcatgggca atcggaaggg atcctgagta ctggcaggac cctttgagtt tcaaaccaga
     1081 aaggtttatc aattccagtg ttgaatataa aggacaacat tttgaattga ttccttttgg
     1141 ttctggaaga agaatttgtg tgggatttcc tttggctcat agagttgttc atctcacttt
     1201 ggctacatta gtccaggctt ttgattggga tcttggtgct ggcgttaaac cacaagatat
     1261 tgatttggaa gagagattgg gattgactct gaggaagaag aatccattga atgtcatccc
     1321 taagaagaga gttcatatct aagcttcgag accctgcagt ccggcaaaaa agggcaaggt
     1381 gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta cttcgcgtta tgcaggcttc
     1441 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     1501 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     1561 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccacag
     1621 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     1681 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     1741 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     1801 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     1861 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     1921 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     1981 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     2041 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     2101 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     2161 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     2221 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     2281 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     2341 aagtatatat gagtaaactt ggtctgacag ctcgaggctt ggattctcac caataaaaaa
     2401 cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg
     2461 atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc tgccactcat
     2521 cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat
     2581 gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca
     2641 tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga
     2701 aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat
     2761 aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga
     2821 aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg
     2881 tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgaa
     2941 attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt
     3001 gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat
     3061 aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata
     3121 tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa
     3181 atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg
     3241 aacctcttac gtgcccgatc aactcgagtg ccacctgacg tctaagaaac cattattatc
     3301 atgacattaa cctataaaaa taggcgtatc acgaggcaga atttcagata aaaaaaatcc
     3361 ttagctttcg ctaaggatga tttctggaat tcggtctcaa atggcgacca tcactttcga
     3421 ttcactaaac ccggtgaccg tcgccatctc agccggtttt cttctcctcc ttatcatctt
     3481 tgtgaaatca agaactggtt catccaaaag aaaaccgccg ggtcctccag gatggccgat
     3541 tttcggcaat atgttcgacc tcggagattt acctcaccaa acgctgtaca agctcaagtc
     3601 caaata
//
