LOCUS       sequence-348992-        3507 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Dudley QM, Jo S, Guerrero DAS, Chhetry M, Smedley MA, Harwood WA, 
            Sherden NH, O'Connor SE, Caputi L, Patron NJ
  TITLE     Reconstitution of monoterpene indole alkaloid biosynthesis in genome
            engineered Nicotiana benthamiana.
  JOURNAL   Commun Biol. 2022 Sep 10;5(1):949. doi: 10.1038/s42003-022-03904-w.
  PUBMED    36088516
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-09-10"; volume: "5(1):949. doi"; pages: " 
            10.1038/s42003-022-03904-w"
COMMENT     Sequence Label: pEPQD0CM0062
FEATURES             Location/Qualifiers
     source          1..3507
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(391..485)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(506..1165)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1080..1099
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1327..1345
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1349..1392)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      2875..2932
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(2956..2973)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(3127..3507,1..208))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3207..3226)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
       61 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
      121 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
      181 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
      241 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
      301 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
      361 gtaaacttgg tctgacagct cgaggcttgg attctcacca ataaaaaacg cccggcggca
      421 accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag
      481 gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcatcg cagtactgtt
      541 gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa
      601 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg
      661 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
      721 gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
      781 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
      841 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
      901 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat tccggatgag
      961 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct
     1021 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag
     1081 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg
     1141 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact
     1201 caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt
     1261 gcccgatcaa ctcgagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     1321 tataaaaata ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt agctttcgct
     1381 aaggatgatt tctggaattc ggtctcaaat gggttctcaa gaaacaaatt tggctcctca
     1441 tgttctcata ttccctttgc caattcaagg ccatgtgaac tccatgctca gactagcaga
     1501 acttctctgt ctagctgaat tagatatcac ttttattgtt tcagagttca gccacagtcg
     1561 cttaattaag catacaaatg ttgcctcccg ttttgcccgt tacccaggat ttcagttcca
     1621 gcccatatcc gatggccttc ccgatgatca ccccagagca ggcgaaagag tcatggatat
     1681 tttgccttca actaaaaatg taacgggacc cctgtttaag cagatgatgg ttgagaataa
     1741 gtgcttttct tctgctactc gaaggccgat tacttgcata atcgctgatg gggttttgag
     1801 ctttgctggg gatttcgctc aagaaaaagg aatccctctt atttacttca gaactgtttc
     1861 tgcttgttct ttctgggctt gtttttgtat gcctgaacta attgaatctg gtgacattcc
     1921 gattaaagga aatggtatgg atctgatagt gaaaagcgtg ccggggatgg aaacttttct
     1981 caggcgacga gatctgccgg ggttttgccg agtcaatgac attaatgaac cgaaactcca
     2041 gatcctgaaa acagagacaa gacagacaac gagagcccaa gcggctattt tgaacacttt
     2101 tgaggatttg gaaggaccaa ttctatctca aattcgcaaa cacatgccaa gacttttcac
     2161 aattggacca agtcattccc acctaacatc tagacttgaa accaagaata tcaagacttt
     2221 aatttcctcc ggtagtttct gggaagaaga tcgaagctgt gtggattggc ttgatgcaca
     2281 gccaccaaga tctgttttat atgtaagttt tggaagtatt acagttgtaa caagagatca
     2341 actcctggaa ttctggtatg gtcttgttaa tagtggacaa cggttcttgt gggtaatgag
     2401 gccagattca attatgggca aagatgggca aagccagatt cctgcagatc ttgaagaggg
     2461 cacaaaagcc agaggttata tggtgggatg ggcacctcaa gaagaggtct tgaatcaccc
     2521 tgccattgga ggatttctga ctcacagtgg gtggaattca actttagaga gtattgtggc
     2581 tggtgtccca atgatatgtt ggccatactt tgcggaccaa atgataaaca gcagatttgt
     2641 gagtgaaatt tggaagattg gattggatat gaaagataca tgtgatagag agacaattgt
     2701 gaagatggtt agggagttga tggagattag gaaagatgag ttcttgcaaa gggctgatca
     2761 tatggctaaa ttggcaaaag aggctgttag tgaaggcgga tcctcatatt ctaacttgga
     2821 tggcctcgtt gattacataa aatcactgat tatttgagct tcgagaccct gcagtccggc
     2881 aaaaaagggc aaggtgtcac caccctgccc tttttcttta aaaccgaaaa gattacttcg
     2941 cgttatgcag gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     3001 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     3061 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     3121 ggcgtttttc cacaggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     3181 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     3241 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     3301 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     3361 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     3421 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     3481 cactggtaac aggattagca gagcgag
//
