LOCUS       sequence-348993-        3600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Dudley QM, Jo S, Guerrero DAS, Chhetry M, Smedley MA, Harwood WA, 
            Sherden NH, O'Connor SE, Caputi L, Patron NJ
  TITLE     Reconstitution of monoterpene indole alkaloid biosynthesis in genome
            engineered Nicotiana benthamiana.
  JOURNAL   Commun Biol. 2022 Sep 10;5(1):949. doi: 10.1038/s42003-022-03904-w.
  PUBMED    36088516
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-09-10"; volume: "5(1):949. doi"; pages: " 
            10.1038/s42003-022-03904-w"
COMMENT     Sequence Label: pEPQD0CM0762
FEATURES             Location/Qualifiers
     source          1..3600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     25..47
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     39..56
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     40..56
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1752..1768)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1752..1768)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1765..1787)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1940..2528)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2020..2039)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2661..2755)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2776..3435)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3350..3369
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(3436..3538)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 atccaccaca agcgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtcatg
       61 cataatccgc acgcatctgg aataaggaag tgccattccg cctgacctag gtctctaatg
      121 gaattgaact tcaaatcaat tattttcttg gtttttgtta gtctaaccct ttactgggtc
      181 tatagaatct tggattgggt atggtttaag cccaagaagc tggaaaaatg cttaagagaa
      241 caagggttta agggaaatcc ttacagattg ttcttgggag atcagtatga tagtggaaaa
      301 ttgatcagac aagccttgac taaacctatt ggtgttgaag aagatgttaa gaaacgaatt
      361 gtacctcata tactcaaaac tgttgggact catggcaaga agtcttttat gtgggttgga
      421 agaataccaa gggtgaatat cacagatcca gagctgatta aagaggtctt aacaaaatac
      481 tataagttcc agaagaatca tcatgatctt gacccaatta ccaaattgct tttgactgga
      541 attggaagct tagaaggtga cccttgggct aaacgccgaa aaatcatcaa tgctgctttt
      601 cactttgaaa agttaaagct tatgcttcct gcattctatt tgagttgccg tgatatggtg
      661 acaaaatggg ataataaggt ccctgaagga ggatcagcag aggtggatgt gtggcatgat
      721 attgaaacct taactggaga tgtaatttca agaacattat ttggaagtaa cttcgaggaa
      781 gggagaagaa tatttgaact catgaaagaa cttactgctc ttactattga tgtcattcgc
      841 tcggtttata tccctggaca gaggtttctt ccaactaaga ggaacaatag gatgagagca
      901 attgacaaag aagttagagt aagaataaca gaaattatca ataagaaaat gaaggtaatg
      961 aaatcaggag aagcagcaag tgctgctgat gattttttgg gaatattgtt ggaatgcaat
     1021 ctgaatgaaa taaaggagca aggaaataac aaaagtgctg gaatgactat tggagagatt
     1081 attggtgagt gcaaactctt ctattttgct ggccaggaca ctacctcgac tttactcgtg
     1141 tggacaatgg tcctcttgtc tagattcccc gaatggcaaa ctcgtgccag agaagaagtt
     1201 tttcaagtct ttggtaataa aaccccggac tatgatggta tcagccatct taaagttata
     1261 acgatgatct tgtacgaggt tttgaggtta tatactccag tggctgaact gacaaaagtg
     1321 gctcatgaag ctacacaact gggaaaatac ttcattccag cgggcgtgca gctaatgatg
     1381 ccacaaatcc tacttcatca tgaccctgaa atatggggtg aagatgtaat ggagttcaaa
     1441 ccagaaagat ttgcagaggg agttctcaag gcaactaaga gccaaggctc tttttttcca
     1501 tttagcttgg gaccaagaat gtgcattggc caaaactttg ctctattgga agccaaaatg
     1561 gcaatgtctc taatcttgcg tagattttct ttcgagcttt ctccctccta tgttcatgct
     1621 cctttcactc tcatcacaat gcaaccccaa tatggtgctc atttgattct gcacaaactc
     1681 taagcttcga gacctaggct aggtggaggc tcagtgatga taagtctgcg atggtggatg
     1741 catgtgtcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcag agggcacaat
     1801 cctattccgc gctatccgac aatctccaag acattaggtg gagttcagtt cggcgtatgg
     1861 catatgtcgc tggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     1921 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     1981 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     2041 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     2101 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     2161 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     2221 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     2281 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     2341 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     2401 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     2461 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     2521 gatctcaaga agatcctttg atcttttcta cggggtctga cgctctattc aacaaagccg
     2581 ccgtcccgtc aagtcagcgt aaatgggtag ggggcttcaa atcgtccccc catacgatat
     2641 aagttgttac tagtgcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt
     2701 ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc
     2761 gagctcgata tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt
     2821 aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg
     2881 catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa
     2941 gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga
     3001 gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca
     3061 cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca
     3121 gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc
     3181 ccatatcacc agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag
     3241 gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt
     3301 taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg
     3361 aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt
     3421 gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac
     3481 gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt gccgatcaac
     3541 gtctcattga tacctgaaac aaaacccatc gtacggccaa ggaagtctcc aataactgtg
//
