LOCUS       sequence-348994-        3150 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Dudley QM, Jo S, Guerrero DAS, Chhetry M, Smedley MA, Harwood WA, 
            Sherden NH, O'Connor SE, Caputi L, Patron NJ
  TITLE     Reconstitution of monoterpene indole alkaloid biosynthesis in genome
            engineered Nicotiana benthamiana.
  JOURNAL   Commun Biol. 2022 Sep 10;5(1):949. doi: 10.1038/s42003-022-03904-w.
  PUBMED    36088516
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-09-10"; volume: "5(1):949. doi"; pages: " 
            10.1038/s42003-022-03904-w"
COMMENT     Sequence Label: pEPQD0CM0763
FEATURES             Location/Qualifiers
     source          1..3150
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     14..36
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     28..45
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     29..45
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1291..1307)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1291..1307)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1304..1326)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1479..2067)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1559..1578)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2200..2294)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2315..2974)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2889..2908
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2975..3077)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 gcgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtcatgc ataatccgca
       61 cgcatctgga ataaggaagt gccattccgc ctgacctagg tctctaatgg ttgccacaat
      121 tgattccatt gaaatgcctg cacttccaac agctgtggaa gcccacccaa tgaaaggtgg
      181 tgatgactcc cacagttact cccaaaattc ttgctaccag aaaggagtaa ttgatgcagc
      241 caaggcagtg attgttgaag cagtgaatga aaaattggat cttgaaaata atccaatctt
      301 tgatccaatc aaacctttcc gcattgcaga ttttggatgt tcaacaggac caaatacttt
      361 ccacgctatg caaaacatag tagaatcagt tgaaacaaaa tacaaatcac ttcaaaaaac
      421 ccctgaattc catgttttct tcaatgatca tgttaacaat gatttcaatg ttctctttag
      481 atcccttcca ccaaacaggg aattttttgc tgctggtgtt cctggatctt tctacactag
      541 agtttttccc aaaaatagca ttcattttgc tcattgttct tatgcacttc attggttatc
      601 taaagtgccc aaggaaattc aagataaaaa ttctttggct tataataagg gaagaattca
      661 ttacactggt actgaaaaac atgttgttaa ggcttatttt ggtcaattcc aaagagattt
      721 tgaagggttt ttaaaagcaa gagctcaaga aattgttgtt ggagggttga tggtgattca
      781 aatacctggg cttcctagtg gtgaagtcct tttctcaagg actggtgctg gtttgcttca
      841 cttccttttg ggaacttcct tgatggaatt ggttaacaag ggaatcatca atgaagaaag
      901 tgtagattcc ttcaatttgc ctcaatatca tccctcagtt gaagatttgg aaatggtgat
      961 agaaatgaac gattgtttca caattgaaag ggttggaact ttacctcatc ccatgaagaa
     1021 tttaccattt gatgttcaaa ggacttcttt acaagtaaga gcaatcatgg aatgcattct
     1081 cactgaacat tttggggaaa atattttgga tccattattt gaaatctaca ccaaaaattt
     1141 gcaagagaat ttccatgttt ttgataagga aattagaaag gatgcagatt tgtaccttgt
     1201 cttgaaacgc aagggaaatt aagcttcgag acctaggcta ggtggaggct cagtgatgat
     1261 aagtctgcga tggtggatgc atgtgtcatg gtcatagctg tttcctgtgt gaaattgtta
     1321 tccgctcaga gggcacaatc ctattccgcg ctatccgaca atctccaaga cattaggtgg
     1381 agttcagttc ggcgtatggc atatgtcgct ggaaagaaca tgtgagcaaa aggccagcaa
     1441 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     1501 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     1561 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     1621 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1681 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1741 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     1801 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     1861 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     1921 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     1981 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     2041 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     2101 gctctattca acaaagccgc cgtcccgtca agtcagcgta aatgggtagg gggcttcaaa
     2161 tcgtcccccc atacgatata agttgttact agtgcttgga ttctcaccaa taaaaaacgc
     2221 ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc
     2281 tatcaacagg agtccaagcg agctcgatat caaattacgc cccgccctgc cactcatcgc
     2341 agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat
     2401 gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg
     2461 tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac
     2521 tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg
     2581 ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat
     2641 cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt
     2701 aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc atacgaaatt
     2761 ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct
     2821 tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg
     2881 tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat
     2941 caacggtggt atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc
     3001 tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac
     3061 ctcttacgtg ccgatcaacg tctcattgat acctgaaaca aaacccatcg tacggccaag
     3121 gaagtctcca ataactgtga tccaccacaa
//
