LOCUS       sequence-348996-        3618 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Dudley QM, Jo S, Guerrero DAS, Chhetry M, Smedley MA, Harwood WA, 
            Sherden NH, O'Connor SE, Caputi L, Patron NJ
  TITLE     Reconstitution of monoterpene indole alkaloid biosynthesis in genome
            engineered Nicotiana benthamiana.
  JOURNAL   Commun Biol. 2022 Sep 10;5(1):949. doi: 10.1038/s42003-022-03904-w.
  PUBMED    36088516
REFERENCE   2  (bases 1 to 3618)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-09-10"; volume: "5(1):949. doi"; pages: " 
            10.1038/s42003-022-03904-w"
COMMENT     Sequence Label: pEPQD0CM0764
FEATURES             Location/Qualifiers
     source          1..3618
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1246..1262)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1246..1262)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1259..1281)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1434..2022)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1514..1533)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2155..2249)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2270..2929)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2844..2863
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2930..3032)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     3119..3141
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3133..3150
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3134..3150
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ccttgttaag ttccttctca ctggagttgg aagctttgaa ggggcaaaat ggtccaaaca
       61 cagaagaatc atttcccctg ccttcactct tgagaaacta aagtcaatgt tgccggcttt
      121 tgccatatgc tatcatgaca tgttgacgaa atgggagaaa ttagcagaaa aagaaggatc
      181 ccatgaagtt gatatcttcc ccacatttga tgttttgaca agtgatgtaa tttcaaaggt
      241 tgcatttggt agcacatacg atgaaggagg caaaatcttc agactattga aagaactcat
      301 ggatctcaca attgattgca tgagagatgt ctacatccca gggtggagct acttgccaac
      361 caagaggaac aagaggatga aagaaattaa caaagaaatc acagatatgc taaggttcat
      421 catcaacaag agaatgaagg ctttgaaggc tggagaacca ggtgaggatg acttgttggg
      481 agtcctgttg gaatcaaaca ttcaagaaat tcagaaacaa ggaaacagga aggatggtgg
      541 aatgacaatc aacgatgtga ttgaggaatg caaattgttc tactttgctg gtcaagaaac
      601 caccggagtt ttgctcacat ggaccaccat tttattgagc aagcatcccg agtggcaaga
      661 gcgagctagg gaagaagttc tccaagcctt tggcaagaac aagcctgaat ttgaacgctt
      721 gaatcatctt aaatatgttt ctatgatctt atacgaggtt ctaaggttgt acccgccagt
      781 tattgatctg accaagatta ttcacgagga cacaaagtta gggccgtaca caatccctgc
      841 aggaacacaa gtaatgttgc cgacagtaat gcttcataga gagaagtcca tttggggaga
      901 agatgcaatg gaattcaacc caatgagatt tgctgatgga gttgccaatg ccaccaagaa
      961 caatgtcaca tatttgccat tcagttgggg acctagagtt tgcctcggcc aaaactttgc
     1021 acttcttcaa gcaaaattag ggttggcaat gattttacaa cgcttcaaat ttgatgtagc
     1081 tccatcctat gttcatgctc cctttaccat tcttacagtt caacctcagt ttggttctca
     1141 tgttatctac aagaagcttg agagacagaa tttttaagct tcgagaccta ggctaggtgg
     1201 aggctcagtg atgataagtc tgcgatggtg gatgcatgtg tcatggtcat agctgtttcc
     1261 tgtgtgaaat tgttatccgc tcagagggca caatcctatt ccgcgctatc cgacaatctc
     1321 caagacatta ggtggagttc agttcggcgt atggcatatg tcgctggaaa gaacatgtga
     1381 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     1441 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     1501 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     1561 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     1621 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     1681 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     1741 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     1801 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     1861 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     1921 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     1981 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     2041 tctacggggt ctgacgctct attcaacaaa gccgccgtcc cgtcaagtca gcgtaaatgg
     2101 gtagggggct tcaaatcgtc cccccatacg atataagttg ttactagtgc ttggattctc
     2161 accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga
     2221 ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat tacgccccgc
     2281 cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat
     2341 cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat
     2401 aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat
     2461 caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc
     2521 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta
     2581 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct
     2641 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca
     2701 ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg
     2761 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa
     2821 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat
     2881 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
     2941 tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat
     3001 ggtgaaagtt ggaacctctt acgtgccgat caacgtctca ttgatacctg aaacaaaacc
     3061 catcgtacgg ccaaggaagt ctccaataac tgtgatccac cacaagcgcc agggttttcc
     3121 cagtcacgac gttgtaaaac gacggccagt catgcataat ccgcacgcat ctggaataag
     3181 gaagtgccat tccgcctgac ctaggtctct aatggagatg gatatggata tcattagaaa
     3241 ggcaattgct gccactatct ttgcattggt tatggcttgg gcgtggagag tgttggattg
     3301 ggcatggttt actcctaaga ggatcgagaa acgtctaagg cagcaaggtt ttagaggaaa
     3361 tccttataga ttcttggttg gagatgttaa ggagagtgga aaaatgcatc aagaagcctt
     3421 gtctaacccc atggagttcg acaacgatat tgttcctcgc ctcatgccac atattaacca
     3481 caccataaaa acttacggta ggaattcatt tacatggatg ggaaggattc caaggattca
     3541 tgttatggaa cctgaactta ttaaggaagt attgacccac tccagcaaat accaaaagaa
     3601 ctttgatgtt cacaatcc
//
