LOCUS       sequence-348997-        3537 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Dudley QM, Jo S, Guerrero DAS, Chhetry M, Smedley MA, Harwood WA, 
            Sherden NH, O'Connor SE, Caputi L, Patron NJ
  TITLE     Reconstitution of monoterpene indole alkaloid biosynthesis in genome
            engineered Nicotiana benthamiana.
  JOURNAL   Commun Biol. 2022 Sep 10;5(1):949. doi: 10.1038/s42003-022-03904-w.
  PUBMED    36088516
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-09-10"; volume: "5(1):949. doi"; pages: " 
            10.1038/s42003-022-03904-w"
COMMENT     Sequence Label: pEPQD0CM0765
FEATURES             Location/Qualifiers
     source          1..3537
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     22..44
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     36..53
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     37..53
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1686..1702)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1686..1702)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1699..1721)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1874..2462)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1954..1973)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2595..2689)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2710..3369)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3284..3303
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(3370..3472)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 caccacaagc gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtcatgcat
       61 aatccgcacg catctggaat aaggaagtgc cattccgcct gacctaggtc tctaatgggc
      121 agcattgatt caacaaatgt agccatgtcc aattctccag ttggagaatt taagccactt
      181 gaagctgagg aattccgaaa acaagcccat cgtatggtag atttcatagc cgattattac
      241 aaaaatgtgg aaacatatcc ggtccttagc gaagtcgaac ctggatatct ccgaaaacgt
      301 atccccgaaa ccgctcctta cctccccgaa ccacttgacg acatcatgaa agatattcag
      361 aaggatatta tcccaggaat gacaaattgg atgagcccta atttttatgc attttttcct
      421 gccactgtta gttcagctgc ctttttagga gaaatgttgt ctactgccct aaattcagta
      481 ggctttactt gggtttcttc accagccgcc accgaattag aaatgattgt tatggattgg
      541 ttggctcaga tccttaaact ccccaaatct ttcatgtttt caggtaccgg tggcggcgtc
      601 atccaaaaca ccactagcga gtccattctt tgtacaatca ttgccgcccg ggaaagggcc
      661 ctggagaagc tcggtcccga tagtattgga aaacttgtct gttacggatc cgatcaaacc
      721 cataccatgt tccccaaaac ttgcaaattg gcgggaattt atccgaataa tattaggtta
      781 atacctacga ccgtcgaaac ggatttcggc atctcacctc aagttctacg aaaaatggtc
      841 gaggatgacg tggcggccgg atatgtaccg ctgttcttat gcgctaccct gggtaccacc
      901 tcgaccacgg ctaccgatcc tgtggactca ctttctgaaa tcgctaacga gtttggtatt
      961 tggatccacg tggatgctgc ttatgcggga agcgcctgta tatgtcccga gtttagacat
     1021 tacttggatg gaatcgaacg agttgactca ctgagtctga gtccacacaa atggctactc
     1081 gcttacttag attgcacttg cttgtgggtc aagcaaccac atttgttact aagggcactc
     1141 actacgaatc ctgagtattt aaaaaataaa cagagtgatt tagacaaagt tgtggacttc
     1201 aaaaattggc aaatcgcaac gggacgaaaa tttcggtcgc tgaaactttg gctcatttta
     1261 cgtagctatg gagttgttaa tttacagagt catattcgtt ctgacgtcgc aatgggcaaa
     1321 atgttcgaag aatgggttag atcagactcc agattcgaaa ttgtggtacc gagaaacttt
     1381 tctcttgttt gttttagatt aaaacctgac gtttcgagtt tacatgtaga agaagtgaat
     1441 aagaaacttt tggacatgct taactcgacg ggacgagttt atatgactca tactattgtg
     1501 ggaggcatat acatgctaag actggctgtt ggctcgtcac taactgaaga acatcatgta
     1561 cgccgtgttt gggatttgat tcaaaaatta accgatgatt tgctcaaaga agcttgagct
     1621 tcgagaccta ggctaggtgg aggctcagtg atgataagtc tgcgatggtg gatgcatgtg
     1681 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcagagggca caatcctatt
     1741 ccgcgctatc cgacaatctc caagacatta ggtggagttc agttcggcgt atggcatatg
     1801 tcgctggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     1861 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     1921 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     1981 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     2041 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     2101 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     2161 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     2221 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     2281 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     2341 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     2401 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     2461 aagaagatcc tttgatcttt tctacggggt ctgacgctct attcaacaaa gccgccgtcc
     2521 cgtcaagtca gcgtaaatgg gtagggggct tcaaatcgtc cccccatacg atataagttg
     2581 ttactagtgc ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac
     2641 aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc
     2701 gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat
     2761 tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag
     2821 caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc
     2881 catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa
     2941 aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac
     3001 atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga
     3061 tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat
     3121 caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc
     3181 aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa
     3241 ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc
     3301 ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt
     3361 tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg
     3421 tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgccgat caacgtctca
     3481 ttgatacctg aaacaaaacc catcgtacgg ccaaggaagt ctccaataac tgtgatc
//
