LOCUS       sequence-348999-        3093 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Dudley QM, Jo S, Guerrero DAS, Chhetry M, Smedley MA, Harwood WA, 
            Sherden NH, O'Connor SE, Caputi L, Patron NJ
  TITLE     Reconstitution of monoterpene indole alkaloid biosynthesis in genome
            engineered Nicotiana benthamiana.
  JOURNAL   Commun Biol. 2022 Sep 10;5(1):949. doi: 10.1038/s42003-022-03904-w.
  PUBMED    36088516
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-09-10"; volume: "5(1):949. doi"; pages: " 
            10.1038/s42003-022-03904-w"
COMMENT     Sequence Label: pEPQD0CM0766
FEATURES             Location/Qualifiers
     source          1..3093
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1003..1019)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1003..1019)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1016..1038)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1191..1779)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1271..1290)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1912..2006)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2027..2686)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2601..2620
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2687..2789)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2876..2898
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2890..2907
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2891..2907
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cccttcctat gctccgaatg ccttcacctt cgattcaact gataaagggt tctacacttc
       61 cgtccaagat ggccgagtta tcaaatatga agggccaaat tcaggcttca ctgacttcgc
      121 ctacgcatct cccttctgga acaaagcttt ttgtgagaac agcaccgatc cagagaaaag
      181 accattgtgt gggaggacat atgatatttc ctatgactat aagaacagcc aaatgtacat
      241 tgttgatggc cattaccatc tttgtgtggt tggaaaagaa ggtgggtatg ccacacaact
      301 agccacaagt gtgcaaggag tgccattcaa atggctctat gcagtaactg ttgatcagag
      361 aacagggatt gtttatttca ctgatgttag ctccatacat gatgacagtc ccgaaggtgt
      421 ggaagaaatc atgaatacaa gtgatagaac agggagatta atgaagtatg atccttcaac
      481 aaaagaaacc accttattat tgaaagagct acatgttccc ggcggtgcag aaatcagcgc
      541 agatggttcc tttgttgtag tagcagaatt tttaagcaat cggatagtga agtattggct
      601 agaagggcca aagaaaggca gtgcagagtt cttagttaca atcccaaatc caggaaatat
      661 aaagaggaat tctgatggcc atttttgggt gtcatctagt gaagaattag atggaggtca
      721 acatggaaga gttgtttcaa gaggaattaa gtttgatgga tttgggaata ttcttcaagt
      781 tataccactt ccaccaccat atgaaggtga acattttgaa cagattcaag agcacgatgg
      841 tttgttatac attggaagtc tcttccatag ctctgtgggt atattagtgt atgatgatca
      901 tgataacaag ggaaattctt atgtttctag ctgagcttcg agacctaggc taggtggagg
      961 ctcagtgatg ataagtctgc gatggtggat gcatgtgtca tggtcatagc tgtttcctgt
     1021 gtgaaattgt tatccgctca gagggcacaa tcctattccg cgctatccga caatctccaa
     1081 gacattaggt ggagttcagt tcggcgtatg gcatatgtcg ctggaaagaa catgtgagca
     1141 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     1201 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     1261 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     1321 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     1381 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     1441 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     1501 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     1561 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     1621 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     1681 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     1741 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     1801 acggggtctg acgctctatt caacaaagcc gccgtcccgt caagtcagcg taaatgggta
     1861 gggggcttca aatcgtcccc ccatacgata taagttgtta ctagtgcttg gattctcacc
     1921 aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt
     1981 cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct
     2041 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac
     2101 aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat
     2161 atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa
     2221 aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt
     2281 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa
     2341 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat
     2401 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg
     2461 ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat
     2521 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg
     2581 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc
     2641 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag
     2701 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt
     2761 gaaagttgga acctcttacg tgccgatcaa cgtctcattg atacctgaaa caaaacccat
     2821 cgtacggcca aggaagtctc caataactgt gatccaccac aagcgccagg gttttcccag
     2881 tcacgacgtt gtaaaacgac ggccagtcat gcataatccg cacgcatctg gaataaggaa
     2941 gtgccattcc gcctgaccta ggtctctaat ggcaaacttt tctgaatcta aatccatgat
     3001 ggcagttttc ttcatgtttt tcctgcttct tttgtcatct tctagttctt catcttcaag
     3061 ttcaccaatt ttgaaaaaga tttttattga aag
//
