LOCUS       sequence-34900-e         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Kamura T, Sato S, Iwai K, Czyzyk-Krzeska M, Conaway RC, Conaway JW
  TITLE     Activation of HIF1alpha ubiquitination by a reconstituted von 
            Hippel-Lindau (VHL) tumor suppressor complex.
  JOURNAL   Proc Natl Acad Sci U S A. 2000 Sep 12. 97(19):10430-5.
  PUBMED    10973499
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2000 Sep 12. 97(19):10430-5."
COMMENT     Sequence Label: pBacPAK8-His-VHL-Flag
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             78..100
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             120..137
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             792..815
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gttttcgtac agttttgtaa taaaaaaacc tataaatacg gatccctgca ggcctcgagt
       61 tcgaatctag aaataatttt gtttaacttt aagaaggaga tatacatatg cggggttctc
      121 atcatcatca tcatcatggt atggctagca tgccccggag ggcggagaac tgggacgagg
      181 ccgaggtagg cgcggaggag gcaggcgtcg aagagtacgg ccctgaagaa gacggcgggg
      241 aggagtcggg cgccgaggag tccggcccgg aagagtccgg cccggaggaa ctgggcgccg
      301 aggaggagat ggaggccggg cggccgcggc ccgtgctgcg ctcggtgaac tcgcgcgagc
      361 cctcccaggt catcttctgc aatcgcagtc cgcgcgtcgt gctgcccgta tggctcaact
      421 tcgacggcga gccgcagccc tacccaacgc tgccgcctgg cacgggccgc cgcatccaca
      481 gctaccgagg tcacctttgg ctcttcagag atgcagggac acacgatggg cttctggtta
      541 accaaactga attatttgtg ccatctctca atgttgacgg acagcctatt tttgccaata
      601 tcacactgcc agtgtatact ctgaaagagc gatgcctcca ggttgtccgg agcctagtca
      661 agcctgagaa ttacaggaga ctggacatcg tcaggtcgct ctacgaagat ctggaagacc
      721 acccaaatgt gcagaaagac ctggagcggc tgacacagga gcgcattgca catcaacgga
      781 tgggggatcc agactacaag gacgatgatg acaagtgaat tcccgggcgg ccgcttaatt
      841 aattgatccg ggttattagt acatttatta agcgctagat tctgtgcgtt gttgattt
//
