LOCUS       sequence-349000-        3183 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Dudley QM, Jo S, Guerrero DAS, Chhetry M, Smedley MA, Harwood WA, 
            Sherden NH, O'Connor SE, Caputi L, Patron NJ
  TITLE     Reconstitution of monoterpene indole alkaloid biosynthesis in genome
            engineered Nicotiana benthamiana.
  JOURNAL   Commun Biol. 2022 Sep 10;5(1):949. doi: 10.1038/s42003-022-03904-w.
  PUBMED    36088516
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-09-10"; volume: "5(1):949. doi"; pages: " 
            10.1038/s42003-022-03904-w"
COMMENT     Sequence Label: pEPQD0CM0910
FEATURES             Location/Qualifiers
     source          1..3183
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(168..262)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(283..942)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     857..876
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1104..1122
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1126..1169)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      2328..2385
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(2409..2426)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2580..3168)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2660..2679)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
       61 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
      121 atctaaagta tatatgagta aacttggtct gacagctcga ggcttggatt ctcaccaata
      181 aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt
      241 actggatcta tcaacaggag tccaagcgag ctcgatatca aattacgccc cgccctgcca
      301 ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc catcacaaac
      361 ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt
      421 gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact
      481 ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa accctttagg
      541 gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg
      601 ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa
      661 aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat
      721 acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa
      781 cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg
      841 gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg
      901 ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc
      961 tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa
     1021 gttggaacct cttacgtgcc cgatcaactc gagtgccacc tgacgtctaa gaaaccatta
     1081 ttatcatgac attaacctat aaaaataggc gtatcacgag gcagaatttc agataaaaaa
     1141 aatccttagc tttcgctaag gatgatttct ggaattcggt ctcaaatggc cccaacactg
     1201 gacaccatcc caactgtaga tgtcaaggaa atgcctgaag aagcgcatcc tatgaaaggc
     1261 ggtgatgact tcaatagcta ttctcagaat tcttgctatc agaggggagt tatagatgct
     1321 gcaaaagtag ttatcgttga agcagtaaat gaaaagctgg atctaaagaa taatgctaat
     1381 tttgatccat ctaagccttt ccacattgct gattttgggt gctcaaccgg ccctaacaca
     1441 tattttgccg tgcaaaatat tgtagaagcc gtggaccaaa aacacaaatc ccttcacgaa
     1501 aaaatccagc cagaattcca tgtttttttc aatgatcatg tcaacaatga tttcaacatt
     1561 ctcttcagat ctctcccaca ggacagggat tattttgctg ctggggtccc tggatcattt
     1621 tacacaaggg tttttcccaa ggccagtgta cattttgctc attgctcgta tgcacttcac
     1681 tggctgtcca aggttcctaa ggaaattcag gacaagaact ctcctgcctt taacagaggg
     1741 agaattcatt acactgggac tgaaaaacat gtcgtgaaag cttacttttc tcaattccaa
     1801 aaagacatgg gctcatttct gaaagctaga gctcaagaaa ttgtgggagg agggttggtg
     1861 gtgattcaaa ttcccggact tccaagtggt gaggtcctct tctctaggac cggtgctggc
     1921 atgcttcatg ctcttttggg atcttccttg atggaactcg taaatttggg aataataacc
     1981 gaagaaagtg tggactcctt caacttgcct caataccatc catcaattga ggaattggaa
     2041 atggtgatag agatgaacaa tagttttacc attgaaaaag ttggagcatt gaatcacccc
     2101 atgaagaact taccatttga cgttcaaatg acttctctgc aaataagagc tgtcatggag
     2161 gggattctgt ctgatcattt tggaggaaaa atcttggatc aattgttcga aatttacacc
     2221 acgaaactgc aagaaaatta caacgttttc gacaaggaaa tccgaaggga tgctgatttg
     2281 ttccttgttc taaaacgtaa gagcaattga gcttcgagac cctgcagtcc ggcaaaaaag
     2341 ggcaaggtgt caccaccctg ccctttttct ttaaaaccga aaagattact tcgcgttatg
     2401 caggcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     2461 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     2521 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     2581 ttccacaggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     2641 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     2701 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     2761 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     2821 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     2881 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     2941 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     3001 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     3061 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     3121 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     3181 atc
//
