LOCUS       sequence-349001-        2340 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 N-terminal tag  part for MoClo assembly, CCAT-AATG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Dudley QM, Jo S, Guerrero DAS, Chhetry M, Smedley MA, Harwood WA, 
            Sherden NH, O'Connor SE, Caputi L, Patron NJ
  TITLE     Reconstitution of monoterpene indole alkaloid biosynthesis in genome
            engineered Nicotiana benthamiana.
  JOURNAL   Commun Biol. 2022 Sep 10;5(1):949. doi: 10.1038/s42003-022-03904-w.
  PUBMED    36088516
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-09-10"; volume: "5(1):949. doi"; pages: " 
            10.1038/s42003-022-03904-w"
COMMENT     Sequence Label: pEPQD0CM0812
FEATURES             Location/Qualifiers
     source          1..2340
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(290..384)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(405..1064)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     979..998
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1226..1244
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1248..1291)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      1607..1664
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1688..1705)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(1859..2340,1..107))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1939..1958)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
       61 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
      121 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
      181 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
      241 caatctaaag tatatatgag taaacttggt ctgacagctc gaggcttgga ttctcaccaa
      301 taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca
      361 ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc cccgccctgc
      421 cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa
      481 acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat
      541 ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa
      601 ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta
      661 gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac
      721 tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg
      781 aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc
      841 atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa
      901 aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc
      961 tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat
     1021 tgggatatat caacggtggt atatccagtg atttttttct ccattttagc ttccttagct
     1081 cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga
     1141 aagttggaac ctcttacgtg cccgatcaac tcgagtgcca cctgacgtct aagaaaccat
     1201 tattatcatg acattaacct ataaaaatag gcgtatcacg aggcagaatt tcagataaaa
     1261 aaaatcctta gctttcgcta aggatgattt ctggaattcg gtctcaccat gacaaattct
     1321 caagcgcttt cacaaccaat tgcctcctct aacgttcatg ataacttcat gaataatgaa
     1381 atcacggcta gtaaaattga tgatggtaat aattcaaaac cactgtcacc tggttggacg
     1441 gaccaaactg cgtataacgc gtttggaatc actacaggga tgtttaatac cactacaatg
     1501 gatgatgtat ataactatct attcgatgat gaagataccc caccaaaacc aaaaaaagag
     1561 ggtggcagtg gagggggcgg aagcggggga atgcgagacc ctgcagtccg gcaaaaaagg
     1621 gcaaggtgtc accaccctgc cctttttctt taaaaccgaa aagattactt cgcgttatgc
     1681 aggcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     1741 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     1801 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     1861 tccacaggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     1921 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     1981 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     2041 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     2101 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     2161 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     2221 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     2281 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
//
