LOCUS       sequence-349003-        3485 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 promoter and 5'UTR part for MoClo assembly, GGAG-CCAT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Dudley QM, Jo S, Guerrero DAS, Chhetry M, Smedley MA, Harwood WA, 
            Sherden NH, O'Connor SE, Caputi L, Patron NJ
  TITLE     Reconstitution of monoterpene indole alkaloid biosynthesis in genome
            engineered Nicotiana benthamiana.
  JOURNAL   Commun Biol. 2022 Sep 10;5(1):949. doi: 10.1038/s42003-022-03904-w.
  PUBMED    36088516
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-09-10"; volume: "5(1):949. doi"; pages: " 
            10.1038/s42003-022-03904-w"
COMMENT     Sequence Label: pUAP12008
FEATURES             Location/Qualifiers
     source          1..3485
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     50..68
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(72..115)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     promoter        1124..1469
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1412..1432
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     terminator      1576..1633
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1657..1674)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1828..2416)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1908..1927)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2599..2693)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2714..3373)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3288..3307
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
       61 cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggaa
      121 ttcggtctca ggaggaattc caatcccaca aaaatctgag cttaacagca cagttgctcc
      181 tctcagagca gaatcgggta ttcaacaccc tcatatcaac tactacgttg tgtataacgg
      241 tccacatgcc ggtatatacg atgactgggg ttgtacaaag gcggcaacaa acggcgttcc
      301 cggagttgca cacaagaaat ttgccactat tacagaggca agagcagcag ctgacgcgta
      361 cacaacaagt cagcaaacag acaggttgaa cttcatcccc aaaggagaag ctcaactcaa
      421 gcccaagagc tttgctaagg ccctaacaag cccaccaaag caaaaagccc actggctcac
      481 gctaggaacc aaaaggccca gcagtgatcc agccccaaaa gagatctcct ttgccccgga
      541 gattacaatg gacgatttcc tctatcttta cgatctagga aggaagttcg aaggtgaagg
      601 tgacgacact atgttcacca ctgataatga gaaggttagc ctcttcaatt tcagaaagaa
      661 tgctgaccca cagatggtta gagaggccta cgcagcaagt ctcatcaaga cgatctaccc
      721 gagtaacaat ctccaggaga tcaaatacct tcccaagaag gttaaagatg cagtcaaaag
      781 attcaggact aattgcatca agaacacaga gaaagacata tttctcaaga tcagaagtac
      841 tattccagta tggacgattc aaggcttgct tcataaacca aggcaagtaa tagagattgg
      901 agtctctaaa aaggtagttc ctactgaatc taaggccatg catggagtct aagattcaaa
      961 tcgaggatct aacagaactc gccgtcaaga ctggcgaaca gttcatacag agtcttttac
     1021 gactcaatga caagaagaaa atcttcgtca acatggtgga gcacgacact ctggtctact
     1081 ccaaaaatgt caaagataca gtctcagaag atcaaagggc tattgagact tttcaacaaa
     1141 ggataatttc gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcgaaa
     1201 ggacagtaga aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcta
     1261 tcattcaaga tctctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca
     1321 tcgtggaaaa agaagaggtt ccaaccacgt ctacaaagca agtggattga tgtgacatct
     1381 ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat
     1441 aaggaagttc atttcatttg gagaggacac gctcgagtat aagagctcat ttttacaaca
     1501 attaccaaca acaacaaaca acaaacaaca ttacaattac atttacaatt atcgataccc
     1561 atcgagaccc tgcagtccgg caaaaaaggg caaggtgtca ccaccctgcc ctttttcttt
     1621 aaaaccgaaa agattacttc gcgttatgca ggcttcctcg ctcactgact cgctgcgctc
     1681 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     1741 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     1801 ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc cgcccccctg acgagcatca
     1861 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     1921 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     1981 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     2041 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     2101 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     2161 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     2221 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     2281 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     2341 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     2401 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     2461 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     2521 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     2581 tgacagctcg aggcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct
     2641 gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga
     2701 gctcgatatc aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa
     2761 gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca
     2821 tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt
     2881 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga
     2941 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg
     3001 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga
     3061 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc
     3121 atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc
     3181 gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta
     3241 aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa
     3301 atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga
     3361 tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc
     3421 ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc ccgatcaact
     3481 cgagt
//
