LOCUS       sequence-349013-        7314 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo Level 1, position 1, transcriptional unit for transient 
            expression of 1-deoxy-D-xylulose 5-phosphate synthase (CrDXS2) from 
            Catharanthus roseus driven by 35S promoter; contains a chloroplast 
            transit peptide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7314)
  AUTHORS   Dudley QM, Jo S, Guerrero DAS, Chhetry M, Smedley MA, Harwood WA, 
            Sherden NH, O'Connor SE, Caputi L, Patron NJ
  TITLE     Reconstitution of monoterpene indole alkaloid biosynthesis in genome
            engineered Nicotiana benthamiana.
  JOURNAL   Commun Biol. 2022 Sep 10;5(1):949. doi: 10.1038/s42003-022-03904-w.
  PUBMED    36088516
REFERENCE   2  (bases 1 to 7314)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-09-10"; volume: "5(1):949. doi"; pages: " 
            10.1038/s42003-022-03904-w"
COMMENT     Sequence Label: pEPQD1CB0107
FEATURES             Location/Qualifiers
     source          1..7314
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    164..188
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        374..719
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     662..682
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     polyA_signal    3061..3235
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    3280..3304
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(3460..4608)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(4944..5532)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5024..5043)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5703..6494,6495..6563))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6326..6345
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6564..6668)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      join(6755..7314,1..72)
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 gtttctggcg cgtttgcagg gccatagacg gccgccagcc cagcggcgag ggcaaccagc
       61 ccggtgagcg tcgcaaagga gatcctgatc tgactgatgg gctgcctgta tcgagtggtg
      121 attttgtgcc gagctgccgg tcggggagct gttggctggc tggtggcagg atatattgtg
      181 gtgtaaacaa attgacgctt agacaactta ataacacatt gcggacgttt ttaatgtact
      241 ggggtggatg cagtgggccc cactctgtga agacaatgcc gaattcggat ccggaggtca
      301 acatggtgga gcacgacact ctggtctact ccaaaaatgt caaagataca gtctcagaag
      361 atcaaagggc tattgagact tttcaacaaa ggataatttc gggaaacctc ctcggattcc
      421 attgcccagc tatctgtcac ttcatcgaaa ggacagtaga aaaggaaggt ggctcctaca
      481 aatgccatca ttgcgataaa ggaaaggcta tcattcaaga tctctctgcc gacagtggtc
      541 ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagaggtt ccaaccacgt
      601 ctacaaagca agtggattga tgtgacatct ccactgacgt aagggatgac gcacaatccc
      661 actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggacac
      721 gctactgtat aagagctcat ttttacaaca attaccaaca acaacaaaca acaaacaaca
      781 ttacaattac atttacaatt atcgataaca tggcttcttc tatgctttct tctgctgctg
      841 ttgttgctac tcgtgctagt gctgctcaag ctagtatggt tgctcctttt actggactta
      901 agtctgctgc ttcttttcct gttactagaa agcaaaacaa ccttgatatt acttctattg
      961 ctagtaacgg aggaagagtt cgagcaatgg ctgctgctca tgggcatgct cataatgttg
     1021 atgaaaatta ccaagtccta gtgaagaagc agcctttgaa tttttcagga gataagcctt
     1081 ttactcctat tttggatacc atcaactacc ctacccatat gaagaatctc tctcttcagg
     1141 agcttcaaca attagctgac gaattaaggg aggagatagt gtattctgtg gccaaaacgg
     1201 gtggtcactt aagttccagc ctaggtgtgg ccgagctcac tgtggcactc caccacgtgt
     1261 tcgaaacccc aaatgataaa atcatatggg atgttggcca ccagacatat gggcataaga
     1321 ttctgacagg gagaaggtca aagatgcata ccatgagaca aacttgcgga cttgcaggtt
     1381 tccccaaaag agatgaaagt gcccatgatg catttggagt tggccatagt tccaccagca
     1441 tttctgccgg tttaggaatg gcaataggac gagatttatt aggtaagaaa aatcatgtaa
     1501 taacggtaat aggagatgga gctatgacag ccggacaagc atatgaagcc atgaataatg
     1561 ctggatatct tgattctaat ctcataatca tattgaatga taacaaacaa gtttctctcc
     1621 cgactgccac aattgatggt cctgcacctc ctactggtgc tctaagcaat gccttaacta
     1681 ggcttcaatc tagtagaaaa tttcgactac ttcgggaggc tgcaaaaggt gtgacaaagc
     1741 aattaggggg acaagttcat gaaatggcag ctaaagtgga tacatatatg agagggatga
     1801 atagccaaag ggcttcctta tttgaagatt taggaatgta ttatattggt ccacttgatg
     1861 gtcacaatat ggaagatctt gttgatattt tagagaaagt aaaatctgtt cctgcaacgg
     1921 gccctgttct tattcatatt attactgaga aaggaaaagg atatcctcct gctgaagctg
     1981 ccgccgacaa aatgcatggg gtggtgaaat ttgagccaat gacaggaaaa caattcaagt
     2041 caaagtcaaa aacaaagtca tatacaaatt attttgctga gagtttaata agtgaagcta
     2101 gggaagatga taagattatt gccattcatg ctgctatggg aggaggaaca ggactcaatt
     2161 tattccagaa acactttcct gaacgatgct ttgatgttgg gatagctgag caacatgctg
     2221 tcacttttgc tgctggctta gcttctgaag gactcaagcc tttttgtgct atttactcat
     2281 ctttcttaca aagaggttat gatcaggtgg ttcatgatgt ggaccttcaa aaaattccag
     2341 taaggtttgc tatggataga gcagggctgg tgggtgcaga tggacccact cattgcggtg
     2401 catttgacac aacttttatg gcttgtttgc ctaatatggt ggtcatggct ccttcagatg
     2461 aaaccgaatt gatgcacatg gttgccacag cagctgccat tgatgatagg cctagttgct
     2521 ttcgatatcc cagaggaaat ggcattggtg caattcttcc accaaacaac aaaggaactc
     2581 cattacagat tggtaaagga agaatattgt tggagggaac aagagttgcc attcttgggt
     2641 atggagcaat ggtgcaaaac tgtttaatgg ctgctcagct tcttgaaatt aatggaatat
     2701 cagctacagt tgttgatgcc cgattttgca aacctcttga tggagatttg attagaaaat
     2761 tggctcaaca acatgaagtc cttatcactg ttgaagaagg atccattggt ggttttggtt
     2821 cccatgtggc acagttcttg gctttgaatg gattgcttga tggaaattta aagtggaggg
     2881 caatgatgct tcctgacaga tatattgatc atggagctca aaatgaccaa attgaagagg
     2941 ctggcctaac tccaaagcat attgctggca ctgtgttttc tctactcagc aaaaacaaga
     3001 atactcttca agttctccag ttttaggctt ctctagctag agtcgatcga caagctcgag
     3061 tttctccata ataatgtgtg agtagttccc agataaggga attagggttc ctatagggtt
     3121 tcgctcatgt gttgagcata taagaaaccc ttagtatgta tttgtatttg taaaatactt
     3181 ctatcaataa aatttctaat tcctaaaacc aaaatccagt actaaaatcc agatcgctgc
     3241 aattgtcttc tgcacgaagt ggtttaaact atcagtgttt gacaggatat attggcgggt
     3301 aaacctaaga gaaaagagcg tttattagaa taatcggata tttaaaaggg cgtgaaaagg
     3361 tttatccgtt cgtccatttg tatgtgcatg ccaaccacag ggttccccag atcaggcgct
     3421 ggctgctgaa cccccagccg gaactgaccc cacaaggccc tagcgtttgc aatgcaccag
     3481 gtcatcattg acccaggcgt gttccaccag gccgctgcct cgcaactctt cgcaggcttc
     3541 gccgacctgc tcgcgccact tcttcacgcg ggtggaatcc gatccgcaca tgaggcggaa
     3601 ggtttccagc ttgagcgggt acggctcccg gtgcgagctg aaatagtcga acatccgtcg
     3661 ggccgtcggc gacagcttgc ggtacttctc ccatatgaat ttcgtgtagt ggtcgccagc
     3721 aaacagcacg acgatttcct cgtcgatcag gacctggcaa cgggacgttt tcttgccacg
     3781 gtccaggacg cggaagcggt gcagcagcga caccgattcc aggtgcccaa cgcggtcgga
     3841 cgtgaagccc atcgccgtcg cctgtaggcg cgacaggcat tcctcggcct tcgtgtaata
     3901 ccggccattg atcgaccagc ccaggtcctg gcaaagctcg tagaacgtga aggtgatcgg
     3961 ctcgccgata ggggtgcgct tcgcgtactc caacacctgc tgccacacca gttcgtcatc
     4021 gtcggcccgc agctcgacgc cggtgtaggt gatcttcacg tccttgttga cgtggaaaat
     4081 gaccttgttt tgcagcgcct cgcgcgggat tttcttgttg cgcgtggtga acagggcaga
     4141 gcgggccgtg tcgtttggca tcgctcgcat cgtgtccggc cacggcgcaa tatcgaacaa
     4201 ggaaagctgc atttccttga tctgctgctt cgtgtgtttc agcaacgcgg cctgcttggc
     4261 ctcgctgacc tgttttgcca ggtcctcgcc ggcggttttt cgcttcttgg tcgtcatagt
     4321 tcctcgcgtg tcgatggtca tcgacttcgc caaacctgcc gcctcctgtt caagacgacg
     4381 cgaacgctcc acggcggccg atggcgcggg cagggcaggg ggagccagtt gcacgctgtc
     4441 gcgctcgatc ttggccgtag cttgctggac catcgagccg acggactgga aggtttcgcg
     4501 gggcgcacgc atgacggtgc ggcttgcgat ggtttcggca tcctcggcgg aaaaccccgc
     4561 gtcgatcagt tcttgcctgt atgccttccg gtcaaacgtc cgattcattc accctccttg
     4621 cgggattgcc ccgactcacg ccggggcaat gtgcccttat tcctgatttg acccgcctgg
     4681 tgccttggtg tccagataat ccaccttatc ggcaatgaag tcggtcccgt agaccgtctg
     4741 gccgtccttc tcgtacttgg tattccgaat cttgccctgc acgaatacca gcgacccctt
     4801 gcccaaatac ttgccgtggg cctcggcctg agagccaaaa cacttgatgc ggaagaagtc
     4861 ggtgcgctcc tgcttgtcgc cggcatcgtt gcgccacatc taggatctgc caggaaccgt
     4921 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     4981 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     5041 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     5101 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     5161 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     5221 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     5281 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     5341 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     5401 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     5461 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     5521 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     5581 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     5641 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     5701 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     5761 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     5821 cccagtgctg caatgatacc gcgagaacca cgctcaccgg ctccagattt atcagcaata
     5881 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     5941 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     6001 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     6061 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     6121 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     6181 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     6241 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     6301 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     6361 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     6421 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     6481 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     6541 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcaa
     6601 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     6661 gttccgcgca cgaattggcc agcgctgcca tttttggggt gaggccgttc gcggccgagg
     6721 ggcgcagccc ctggggggat gggaggcccg cgttagcggg ccgggagggt tcgagaaggg
     6781 ggggcacccc ccttcggcgt gcgcggtcac gcgcacaggg cgcagccctg gttaaaaaca
     6841 aggtttataa atattggttt aaaagcaggt taaaagacag gttagcggtg gccgaaaaac
     6901 gggcggaaac ccttgcaaat gctggatttt ctgcctgtgg acagcccctc aaatgtcaat
     6961 aggtgcgccc ctcatctgtc agcactctgc ccctcaagtg tcaaggatcg cgcccctcat
     7021 ctgtcagtag tcgcgcccct caagtgtcaa taccgcaggg cacttatccc caggcttgtc
     7081 cacatcatct gtgggaaact cgcgtaaaat caggcgtttt cgccgatttg cgaggctggc
     7141 cagctccacg tcgccggccg aaatcgagcc tgcccctcat ctgtcaacgc cgcgccgggt
     7201 gagtcggccc ctcaagtgtc aacgtccgcc cctcatctgt cagtgagggc caagttttcc
     7261 gcgaggtatc cacaacgccg gcggccgcgg tgtctcgcac acggcttcga cggc
//
