LOCUS       sequence-349021-       10717 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the firefly luciferase gene, E2A, tdTomato, P2A, and the 
            neomycin resistance gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10717)
  AUTHORS   Mustafa EH, Laven-Law G, Kikhtyak Z, Nguyen V, Ali S, Pace AA, Iggo 
            R, Kebede A, Noll B, Wang S, Winter JM, Dwyer AR, Tilley WD, Hickey 
            TE
  TITLE     Selective inhibition of CDK9 in triple negative breast cancer.
  JOURNAL   Oncogene. 2023 Nov 24. doi: 10.1038/s41388-023-02892-3.
  PUBMED    38001268
REFERENCE   2  (bases 1 to 10717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncogene. 
            2023 Nov 24. doi: 10.1038/s41388-023-02892-3."
COMMENT     Sequence Label: pDRM18 LTN (Luciferase tdTomato Neo)
FEATURES             Location/Qualifiers
     source          1..10717
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..350
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    843..1076
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1261..1305
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1572..1689
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      2291..2300
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2297..3946
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(2363..2381)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     CDS             3956..4015
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     regulatory      4028..4037
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4034..5461
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             5471..5527
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5528..6319
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5579..5598)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6189..6208
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    6339..6927
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6392..6412)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6810..6821)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7014..7247
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7319..7440
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7356..7375)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7410..7429
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7480..7615
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7635..7654)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7636..7654)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7664..7681)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7664..7680)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7673..7695)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7822..8275
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7909..7928)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8117..8138
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8301..8405
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8406..8474,8475..9266)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8624..8643)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9437..10025
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9926..9945
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10179..10196
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10313..10334
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10346..10351,10352..10369,10370..10376)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10384..10400
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10389..10411
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10408..10424
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10408..10424
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10443..10463
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10445..10463
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10491..10717
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcgactggtg agtacgccaa
      301 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      361 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
      421 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
      481 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
      541 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
      601 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
      661 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
      721 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
      781 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
      841 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
      901 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
      961 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1021 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1081 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1141 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1201 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1261 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1321 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1381 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     1441 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     1501 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     1561 tatcggttaa cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     1621 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     1681 caaaatttta tcgatcacga gactagcctc gaggatatcg agatcgatta gtccaatttg
     1741 ttaaagacag gatatcagtg gtccaggctc tagttttgac tcaacaatat caccagctga
     1801 agcctataga gtacgagcca tagatagaat aaaagatttt atttagtctc cagaaaaagg
     1861 ggggaatgaa agaccccacc tgtaggtttg gcaagctagg atcaaggtta ggaacagaga
     1921 gacagcagaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg
     1981 gccaagaaca gttggaacag cagaatatgg gccaaacagg atatctgtgg taagcagttc
     2041 ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtcccgcc ctcagcagtt
     2101 tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt
     2161 atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc
     2221 tcaataaaag agcccacaac ccctcactcg gcgcgatcga tgaattcgag ctcggtaccc
     2281 ggggatccaa gccaccatgg aagacgccaa aaacataaag aaaggcccgg cgccattcta
     2341 tccgctggaa gatggaaccg ctggagagca actgcataag gctatgaaga gatacgccct
     2401 ggttcctgga acaattgctt ttacagatgc acatatcgag gtggacatca cttacgctga
     2461 gtacttcgaa atgtccgttc ggttggcaga agctatgaaa cgatatgggc tgaatacaaa
     2521 tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc
     2581 gttatttatc ggagttgcag ttgcgcccgc gaacgacatt tataatgaac gtgaattgct
     2641 caacagtatg ggcatttcgc agcctaccgt ggtgttcgtt tccaaaaagg ggttgcaaaa
     2701 aattttgaac gtgcaaaaaa agctcccaat catccaaaaa attattatca tggattctaa
     2761 aacggattac cagggatttc agtcgatgta cacgttcgtc acatctcatc tacctcccgg
     2821 ttttaatgaa tacgattttg tgccagagtc cttcgatagg gacaagacaa ttgcactgat
     2881 catgaactcc tctggatcta ctggtctgcc taaaggtgtc gctctgcctc atagaactgc
     2941 ctgcgtgaga ttctcgcatg ccagagatcc tatttttggc aatcaaatca ttccggatac
     3001 tgcgatttta agtgttgttc cattccatca cggttttgga atgtttacta cactcggata
     3061 tttgatatgt ggatttcgag tcgtcttaat gtatagattt gaagaagagc tgtttctgag
     3121 gagccttcag gattacaaga ttcaaagtgc gctgctggtg ccaaccctat tctccttctt
     3181 cgccaaaagc actctgattg acaaatacga tttatctaat ttacacgaaa ttgcttctgg
     3241 tggcgctccc ctctctaagg aagtcgggga agcggttgcc aagaggttcc atctgccagg
     3301 tatcaggcaa ggatatgggc tcactgagac tacatcagct attctgatta cacccgaggg
     3361 ggatgataaa ccgggcgcgg tcggtaaagt tgttccattt tttgaagcga aggttgtgga
     3421 tctggatacc gggaaaacgc tgggcgttaa tcaaagaggc gaactgtgtg tgagaggtcc
     3481 tatgattatg tccggttatg taaacaatcc ggaagcgacc aacgccttga ttgacaagga
     3541 tggatggcta cattctggag acatagctta ctgggacgaa gacgaacact tcttcatcgt
     3601 tgaccgcctg aagtctctga ttaagtacaa aggctatcag gtggctcccg ctgaattgga
     3661 atccatcttg ctccaacacc ccaacatctt cgacgcaggt gtcgcaggtc ttcccgacga
     3721 tgacgccggt gaacttcccg ccgccgttgt tgttttggag cacggaaaga cgatgacgga
     3781 aaaagagatc gtggattacg tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg
     3841 agttgtgttt gtggacgaag taccgaaagg tcttaccgga aaactcgacg caagaaaaat
     3901 cagagagatc ctcataaagg ccaagaaggg cggaaagatc gccgtggcta gcggacagtg
     3961 tactaattat gctctcttga aattggctgg agatgttgag agcaaccctg gacctcatat
     4021 accggtcgcc accatggtga gcaagggcga ggaggtcatc aaagagttca tgcgcttcaa
     4081 ggtgcgcatg gagggctcca tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     4141 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggcg gccccctgcc
     4201 cttcgcctgg gacatcctgt ccccccagtt catgtacggc tccaaggcgt acgtgaagca
     4261 ccccgccgac atccccgatt acaagaagct gtccttcccc gagggcttca agtgggagcg
     4321 cgtgatgaac ttcgaggacg gcggtctggt gaccgtgacc caggactcct ccctgcagga
     4381 cggcacgctg atctacaagg tgaagatgcg cggcaccaac ttcccccccg acggccccgt
     4441 aatgcagaag aagaccatgg gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg
     4501 cgtgctgaag ggcgagatcc accaggccct gaagctgaag gacggcggcc actacctggt
     4561 ggagttcaag accatctaca tggccaagaa gcccgtgcaa ctgcccggct actactacgt
     4621 ggacaccaag ctggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
     4681 gcgctccgag ggccgccacc acctgttcct ggggcatggc accggcagca ccggcagcgg
     4741 cagctccggc accgcctcct ccgaggacaa caacatggcc gtcatcaaag agttcatgcg
     4801 cttcaaggtg cgcatggagg gctccatgaa cggccacgag ttcgagatcg agggcgaggg
     4861 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggcggccc
     4921 cctgcccttc gcctgggaca tcctgtcccc ccagttcatg tacggctcca aggcgtacgt
     4981 gaagcacccc gccgacatcc ccgattacaa gaagctgtcc ttccccgagg gcttcaagtg
     5041 ggagcgcgtg atgaacttcg aggacggcgg tctggtgacc gtgacccagg actcctccct
     5101 gcaggacggc acgctgatct acaaggtgaa gatgcgcggc accaacttcc cccccgacgg
     5161 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc accgagcgcc tgtacccccg
     5221 cgacggcgtg ctgaagggcg agatccacca ggccctgaag ctgaaggacg gcggccacta
     5281 cctggtggag ttcaagacca tctacatggc caagaagccc gtgcaactgc ccggctacta
     5341 ctacgtggac accaagctgg acatcacctc ccacaacgag gactacacca tcgtggaaca
     5401 gtacgagcgc tccgagggcc gccaccacct gttcctgtac ggcatggacg agctgtacaa
     5461 gggaagcgga gctactaact tcagcctgct gaagcaggct ggagacgtgg aggagaaccc
     5521 tggacctatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     5581 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     5641 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     5701 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     5761 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     5821 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     5881 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     5941 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     6001 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc
     6061 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     6121 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     6181 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     6241 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     6301 tcttgacgag ttcttctgat cgatactcga tagtcgacaa tcaacctctg gattacaaaa
     6361 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     6421 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     6481 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     6541 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     6601 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     6661 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     6721 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
     6781 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     6841 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     6901 ggatctccct ttgggccgcc tccccgcctg gaattcgagc tcggtacctt taagaccaat
     6961 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     7021 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
     7081 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     7141 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     7201 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     7261 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     7321 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     7381 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     7441 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
     7501 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     7561 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg
     7621 acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac
     7681 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     7741 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     7801 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     7861 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     7921 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     7981 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     8041 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     8101 accacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     8161 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     8221 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     8281 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     8341 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     8401 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     8461 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     8521 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     8581 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     8641 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     8701 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     8761 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     8821 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     8881 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     8941 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     9001 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     9061 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     9121 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     9181 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     9241 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     9301 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     9361 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     9421 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     9481 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     9541 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     9601 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     9661 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     9721 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     9781 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     9841 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     9901 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     9961 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    10021 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    10081 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
    10141 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
    10201 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
    10261 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
    10321 gttagctcac tcattaggcc caggctttac actttatgct tccggctcgt atgttgtgtg
    10381 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
    10441 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
    10501 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
    10561 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
    10621 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
    10681 agatattgta tttaagtgcc tagctcgata cataaac
//
